A5029679472- Genetic diversity and population structure
- Wildlife Ecology and Conservation
- Species Distribution and Climate Change
- CRISPR and Genetic Engineering
- Geology and Paleoclimatology Research
- Spectroscopy and Laser Applications
- Microbial Metabolic Engineering and Bioproduction
- Advanced Chemical Physics Studies
- Cryospheric studies and observations
- Viral Infectious Diseases and Gene Expression in Insects
- Bat Biology and Ecology Studies
- Genomics and Phylogenetic Studies
- earthquake and tectonic studies
- Bacterial Genetics and Biotechnology
- Ecology and Vegetation Dynamics Studies
- Geological and Geochemical Analysis
- Bacteriophages and microbial interactions
- Evolution and Paleontology Studies
- Photochemistry and Electron Transfer Studies
- High-pressure geophysics and materials
- Assembly Line Balancing Optimization
- Enzyme Structure and Function
- Rangeland and Wildlife Management
- RNA and protein synthesis mechanisms
- Identification and Quantification in Food
Center for Excellence in Molecular Plant Sciences
2009-2025
Chinese Academy of Sciences
2014-2025
Kunming Institute of Zoology
2015-2025
Qufu Normal University
2024
Hefei Institutes of Physical Science
2024
Anhui Institute of Optics and Fine Mechanics
2022
Anhui University
2022
Shanghai Institutes for Biological Sciences
2009-2021
University of Chinese Academy of Sciences
2012-2021
High Magnetic Field Laboratory
2019
Abstract Corynebacterium glutamicum is an important industrial metabolite producer that difficult to genetically engineer. Although the Streptococcus pyogenes ( Sp ) CRISPR-Cas9 system has been adapted for genome editing of multiple bacteria, it cannot be introduced into C. . Here we report a Francisella novicida Fn CRISPR-Cpf1-based genome-editing method CRISPR-Cpf1, combined with single-stranded DNA (ssDNA) recombineering, precisely introduces small changes bacterial at efficiencies...
Controlling the copy number of gene expression cassettes is an important strategy to engineer bacterial cells into high-efficiency biocatalysts. Current strategies mostly use plasmid vectors, but multicopy plasmids are often genetically unstable, and their numbers cannot be precisely controlled. The integration a chromosome has advantages, iterative laborious, it challenging obtain library with varied doses for phenotype characterization. Here, we demonstrated that chromosomal using...
Efficient and novel recombinant protein expression systems can further reduce the production cost of enzymes. Vibrio natriegens is fastest growing free-living bacterium with a doubling time less than 10 min, which makes it highly attractive as host. Here, 196 pET plasmids different genes interest (GOIs) were electroporated into V. strain VnDX, carries an integrated T7 RNA polymerase cassette. As result, 65 75% tested GOIs obtained soluble in Escherichia coli , respectively, 20 showed better...
Both anthropogenic impacts and historical climate change could contribute to population decline species extinction, but their relative importance is still unclear. Emerging approaches based on genomic, climatic data provide a promising analytical framework address this question. This study applied such an integrative approach examine potential drivers for the endangerment of green peafowl (Pavo muticus). Several demographic reconstructions genomes congruently retrieved drastic declination...
l-Proline is an important amino acid that has various industrial applications. Industrial l-proline-producing strains are obtained by the mutagenesis of Corynebacterium glutamicum. In this study, optimized C. glutamicum genome-editing tools were further applied in de novo construction a hyper-l-proline-producing strain. Overexpression feedback inhibition-resistant γ-glutamic kinase mutant ProBG149K, deletion proline dehydrogenase to block l-proline degradation, overexpression glutamate...
Abstract Background Individual organisms may exhibit phenotypic plasticity when they acclimate to different conditions. Such plastic responses facilitate or constrain the adaptation of their descendant populations new environments, complicating evolutionary trajectories beyond genetic blueprint. Intriguingly, itself can evolve in terms its direction and magnitude during adaptation. However, we know little about what determines evolution plasticity, including gene expression plasticity....
The preferred method for Escherichia coli genome editing relies on Cas9 from Streptococcus pyogenes (SpCas9) and λ-Red recombinase. Although SpCas9 is currently the most active RNA-guided DNA endonuclease, a significant number of escapers are often observed, making it inefficient across different sites, particularly when inserting large fragments. In this study, we identified two RAGATH RNA-associated endonucleases (RADs) derived IS607 transposons. Both them exhibited high cleavage activity...
Corynebacterium glutamicum serves as a key microbial chassis for the industrial production of feed and food ingredients. While long DNA fragment insertion technologies have advanced strain engineering capabilities, previous approaches such utilizing chromosome-integrated Cas9-RecET system were constrained by maximum size 7.5 kb. Through systematic evaluation Cas9, gRNA, recombinase expression driven five distinct promoters their implementation on 1 or 2 plasmids with compatible replicons...
The glacial-interglacial cycles in the Pleistocene caused repeated range expansion and contraction of species several regions world. However, it remains uncertain whether such climate oscillations had similar impact on East Asian biota, despite its widely recognized importance global biodiversity. Here we use both molecular ecological niche profiles 11 avian with various elevational ranges to reveal their response late changes. models (ENM) consistently showed that these might substantially...
We studied the phylogeography and population history of white wagtail Motacilla alba , which has a vast breeding range, covering areas with different Pleistocene climatic histories. The mitochondrial NADH dehydrogenase subunit II gene (ND2) Control Region (CR) were analyzed for 273 individuals from 45 localities. Our data comprised all nine subspecies wagtail. Four primary clades inferred (M, N, SW SE), indications M. grandis being nested within . oldest split was between two haplotypes...
Species-level paraphyly inferred from mitochondrial gene trees is a prevalent phenomenon in taxonomy and systematics, but there are several potential causes that not easily explained by currently used methods. This study aimed to test the underlying behind observed of Streak-breasted Scimitar Babbler (Pomatorhinus ruficollis) via statistical analyses four (mtDNA) nine nuclear (nuDNA) genes. Mitochondrial show P. ruficollis with respect Taiwan musicus), genealogies support sister-group...
Mounting observational records demonstrate human-caused faunal decline in recent decades, while accumulating archaeological evidence suggests an early biodiversity impact of human activities during the Holocene. A fundamental question arises concerning whether modern wildlife population declines began disturbance. Here, we performed a genomic analysis six common forest birds East Asia to address this question. For five them, demographic history inference based on 25-33 genomes each species...
Abstract In birds, widespread species complexes often exhibit dramatic plumage differences across their distribution, which can give rise to discordance between morphotypes and phylogroups. Accurate identification of diversity may require an integrated approach in multilocus genetic data are used for inference further corroborated by ecological, morphometric or behavioural data. Pomatorhinus gravivox P. swinhoei , were formerly considered component races erythrogenys recently split using a...