A5100430679- CRISPR and Genetic Engineering
- Solid State Laser Technologies
- Marine and coastal ecosystems
- Aquatic Ecosystems and Phytoplankton Dynamics
- Advanced Fiber Laser Technologies
- Metal-Organic Frameworks: Synthesis and Applications
- Cancer therapeutics and mechanisms
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- Polysaccharides and Plant Cell Walls
- Metabolomics and Mass Spectrometry Studies
- Laser-Matter Interactions and Applications
- Carbon dioxide utilization in catalysis
- Mass Spectrometry Techniques and Applications
- Semiconductor Quantum Structures and Devices
- CO2 Reduction Techniques and Catalysts
- Computational Drug Discovery Methods
- ZnO doping and properties
- Arctic and Antarctic ice dynamics
- Advancements in Semiconductor Devices and Circuit Design
- Bioactive Compounds and Antitumor Agents
- Catalytic Processes in Materials Science
- RNA and protein synthesis mechanisms
- Marine Toxins and Detection Methods
- Algal biology and biofuel production
Guangxi Academy of Sciences
2025
Ministry of Public Security of the People's Republic of China
2025
Institute of Forensic Science
2025
Zhejiang University of Technology
2025
Southern Medical University
2024
Dalian University of Technology
2023-2024
Fujian Agriculture and Forestry University
2024
Qingdao Institute of Bioenergy and Bioprocess Technology
2024
Chinese Academy of Sciences
2014-2024
Qinghai New Energy (China)
2024
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we established previously has been widely used in Escherichia coli MG1655. However, this failed to manipulate the of E. BL21(DE3), owing potential higher leaky transcription gRNA-pMB1 specific pTargetF strain. In study, modified by replacing promoter with a tightly regulated PrhaB, changing replicon pCas nontemperature-sensitive replicon,...
Controlling the copy number of gene expression cassettes is an important strategy to engineer bacterial cells into high-efficiency biocatalysts. Current strategies mostly use plasmid vectors, but multicopy plasmids are often genetically unstable, and their numbers cannot be precisely controlled. The integration a chromosome has advantages, iterative laborious, it challenging obtain library with varied doses for phenotype characterization. Here, we demonstrated that chromosomal using...
As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, polysaccharides play important roles against owing to their abundant bioactivities. In this paper, antioxidant anti-aging activities of Flammulina velutipes (FPS) its sulfated FPS (SFPS) on d-galactose-induced mice were investigated. The in vitro demonstrated that SFPS had strong reducing power superior scavenging effects 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl...
Abstract Wild yak ( Bos mutus ) and domestic grunniens are adapted to high altitude environment have ecological, economic, cultural significances on the Qinghai-Tibetan Plateau (QTP). Currently, genetic cellular bases underlying adaptations of extreme conditions remains elusive. In present study, we assembled two chromosome-level genomes, one each for wild yak, screened structural variants (SVs) through long-read data taurine cattle. The results revealed that 6733 genes contained high-FST...
Abstract During the past decade, cognitive neuroscience has been calling for population diversity to address challenge of validity and generalizability, ushering in a new era neuroscience. The developing Chinese Color Nest Project (devCCNP, 2013–2022), first ten-year stage lifespan CCNP (2013–2032), is two-stages project focusing on brain-mind development. aims create share large-scale, longitudinal multimodal dataset typically children adolescents (ages 6.0–17.9 at enrolment) population....
Abstract Cell engineering is commonly limited to the serial manipulation of a single gene or locus. The recently discovered CRISPR-associated transposases (CASTs) could manipulate multiple sets genes achieve predetermined cell diversity, with orthogonal CASTs being able them in parallel. Here, novel CAST from Pseudoalteromonas translucida KMM520 (PtrCAST) was characterized without protospacer adjacent motif (PAM) preference which can high insertion efficiency for larger cargo and multiplexed...
The efficient conversion of methane into methanol at low temperature under pressure remains a great challenge largely because the inertness and poor solubility methane. Herein, we report that porous organic polymer-supported Pd catalyst, which was constructed via Friedel–Crafts type polymerization between 4,6-dichloropyrimidine 1,3,5-triphenyl benzene subsequent metalation, enabled to methyl trifluoroacetate, precursor methanol, atmosphere (1 atm) 80 °C afford 51% yield relative with TON 664...
We develop a method to fabricate an undoped Ge quantum well (QW) under 32 nm relaxed Si0.2Ge0.8 shallow barrier. The bottom barrier contains (650 °C) and Si0.1Ge0.9 (800 such that variation of content forms sharp interface can suppress the threading dislocation density (TDD) penetrating into well. SiGe introduces enough in-plane parallel strain (ε∥ -0.41%) in heterostructure field-effect transistors with buried channel obtain ultrahigh two-dimensional hole gas (2DHG) mobility over 2 × 106...
Skeletonema costatum and Prorocentrum donghaiense co-exist appear alternately as dominant species in the natural phytoplankton community coastal area of East China Sea. The mechanism that determines which two dominates succession remains unknown. We conducted a series experiments to investigate inter-specific relationship between S. P. donghaiense. results bialgal culture indicated under high-nutrient conditions, interaction was independent initial chlorophyll ratios. Adding filtrates did...
Lipid quantification is the ultimate goal in lipidomics studies challenged by availability of standard compounds. A novel strategy for targeted based on LC-MS/MS parameters prediction and multivariate statistical analysis was developed quantitation lysophosphatidylcholines (LPCs) this study. Multiple linear regression models were established with acyl chain length number double bonds after correlation coefficients (R2pred) evaluated. Then related analytical including collision energy,...
Microorganisms are essential for the formation of biogenic coalbed methane (CBM) and application microbially enhanced CBM (MECoM). Anthracite-degrading methanogenic microflora was cultivated by enrichment subculture from produced water obtained in Qinshui Basin where is explored commercially. The maximum yield 255 μmol/g coal after 23 days cultivation at 35 °C, 1.1–1.2% salinity, <0.15 mm particle size, pH 8–9, 5 mL inocula/g coal. Microflora archaea, as revealed MiSeq, were mainly composed...
The development of green solid polymer materials bearing multiple active sites as highly efficient heterogeneous catalysts with cooperative effects toward the conversion carbon dioxide (CO2) to high-value-added cyclic carbonate is in need. Here a novel cationic based on moieties 1-bromomethyl-4-hydroxy calix[4]arene and tri(4-imidazolylphenyl)amine has been synthesized, characterized, used metal-free bifunctional catalyst for CO2 utilization. presence abundant phenolic OH groups hydrogen...
This study aimed to identify novel peptides with antioxidant, anti-lipoxygenase (LOX), anti-xanthine oxidase (XOD), and anti-tyrosinase activities from velvet antler blood (VB) treated simulated gastrointestinal digestion. VB hydrolysate (VBH) was subjected anti-LOX, anti-XOD, assay-guided purification using ultrafiltration Sephadex G-25 gel filtration chromatography. Two-hundred nineteen different peptide sequences were identified the most active fraction nano-LC-MS/MS mainly derived serum...
Formic acid (FA), a sustainable and safe hydrogen storage vector, has the advantages of nontoxicity, high content (4.4 wt %) low cost. However, dehydrogenation formic at near room temperature remains big challenge in terms favorable release rate CO-absence production. Herein, series nitrogen-decorated carbon nanosheets (n-CNS) supported AuPd nanoparticles (NPs) were designed employed as efficient catalysts to dehydrogenate FA for first time. The catalyst NPs on n-CNS synthesized hydrothermal...
Directed evolution and targeted genome editing have been deployed to create genetic variants with usefully altered phenotypes. However, these methods are limited high-throughput screening or serial manipulation of single genes. In this study, we implemented multicopy chromosomal integration using CRISPR-associated transposases (MUCICAT) simultaneously target up 11 sites on the Escherichia coli chromosome for multiplex gene interruption and/or insertion, generating combinatorial genomic...