A5007230817- Reproductive Biology and Fertility
- DNA Repair Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ovarian function and disorders
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Reproductive System and Pregnancy
- Renal and related cancers
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Sexual Differentiation and Disorders
- MicroRNA in disease regulation
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Endometriosis Research and Treatment
- Circular RNAs in diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- BRCA gene mutations in cancer
- Ovarian cancer diagnosis and treatment
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
- Cytokine Signaling Pathways and Interactions
- Mitochondrial Function and Pathology
Shandong University
2016-2025
Shandong Provincial Hospital
2015-2025
Shandong First Medical University
2023-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024-2025
Shandong Maternal and Child Health Hospital
2023-2024
Shandong Women’s University
2024
State Key Laboratory of Reproductive Medicine
2023-2024
Chinese University of Hong Kong
2019-2020
Ministry of Education of the People's Republic of China
2011-2015
Second Hospital of Shandong University
2012
Abstract Premature ovarian insufficiency (POI) is a major cause of female infertility due to early loss function. POI heterogeneous condition, and its molecular etiology unclear. To identify genetic variants associated with POI, here we performed whole-exome sequencing in cohort 1,030 patients POI. We detected 195 pathogenic/likely pathogenic 59 known POI-causative genes, accounting for 193 (18.7%) cases. Association analyses comparing the control 5,000 individuals without identified 20...
Ovarian granulosa cells (GCs) proliferate and differentiate along with follicular growth, this is indispensable for oocyte development female fertility. Although the role of macroautophagy/autophagy in ovarian function has been reported, its contribution to regulation GC characteristics remains elusive. The siRNA-mediated knockdown two key autophagy-related genes ATG5 BECN1 autophagy inhibitor chloroquine were used interfere GCs. Inhibition both genetically pharmacologically resulted...
Diets rich in SFA have been implicated Alzheimer's disease (AD). There is strong evidence to suggest that microglial activation augments the progression of AD. However, it remains uncertain whether can initiate and this response cause neuronal death. Using BV-2 cell line primary culture, we showed palmitic acid (PA) stearic (SA) could activate microglia, as assessed by reactive morphological changes significantly increased secretion pro-inflammatory cytokines, NO oxygen species, which...
Guo Y, Liu S, Wang P, Zhao F, Bing L, Zhang Ling E-A, Gao J & Hao A(2011) Histopathology59, 763–775 Expression profile of embryonic stem cell-associated genes Oct4, Sox2 and Nanog in human gliomas Aims: To investigate whether Nanog, three core regulatory factors maintaining pluripotency self-renewal cells (ESCs), are coexpressed gliomas, their expression might be linked to carcinogenesis the formation cancer (CSCs). Methods results: Forty cases glioma were examined. The was analysed by...
Primary ovarian insufficiency (POI) is a genetically heterogeneous disorder that occurs in familial or sporadic fashion. Through whole exome sequencing Chinese pedigree with POI, we identified novel homozygous missense mutation (ENST00000375755: c.1459G > T, p.D487Y) the MSH5 gene two sisters POI. The homologous mice resulted atrophic ovaries without oocytes, and vitro functional study revealed mutant impaired DNA recombination repair. From sanger of 200 POI patients, three heterozygous...
Abstract The genetic etiology of premature ovarian insufficiency (POI) has been well established to date, however, the role long noncoding RNAs (lncRNAs) in POI is largely unknown. In this study, we identified a down-expressed lncRNA HCP5 granulosa cells (GCs) from biochemical (bPOI) patients, which impaired DNA damage repair and promoted apoptosis GCs. Mechanistically, discovered that stabilized interaction between YB1 its partner ILF2, could mediate transferring into nucleus silencing...
Abstract Background Declining ovarian function in advance-aged women and premature insufficiency (POI) patients seriously affects quality of life, there is currently no effective treatment to rescue clinic. Stem cell transplantation a promising therapeutic strategy for aging, but its clinical application limited due the low efficiency unclear mechanism. Here, novel combination umbilical cord-mesenchymal stem cells (UC-MSCs) autocrosslinked hyaluronic acid (HA) gel explored reserve fecundity...
Melatonin, an endogenously produced neurohormone secreted by the pineal gland, has a variety of physiological functions and neuroprotective effects. It can modulate neural stem cells (NSCs) including proliferation differentiation in embryonic brain tissue but its effect mechanism on hypoxia remains to be explored. Here, we show that melatonin stimulates NSCs during hypoxia. Additionally, it also promoted into neurons. However, did not appear exert obvious astrocytes. The present results have...
Premature ovarian failure (POF) is a rare, heterogeneous disorder characterized by cessation of menstruation occurring before the age 40 years. Genetic etiology responsible for perhaps 25% cases, but most cases are sporadic and unexplained. In this study, through whole exome sequencing in non-consanguineous family having four affected members with POF Sanger 432 we identified three novel mutations fusion gene CSB-PGBD3. Subsequently functional studies suggest that mutated CSB-PGBD3 protein...
Abstract Immune dysregulation has long been proposed as a component of premature ovarian insufficiency (POI), but the underlying mediators and mechanisms remain largely unknown. Here we showed that patients with POI had augmented T helper 1 (T H 1) responses regulatory reg ) cell deficiency in both periphery ovary compared to control women. The increased ratio 1:T cells was strongly correlated severity POI. In mouse models POI, infiltration resulted follicle atresia insufficiency, which...
Abstract Primordial follicle activation is fundamental for folliculogenesis and the maintenance of fertility. An effective therapeutic strategy patients with premature ovarian insufficiency (POI) to promote residual primordial follicles. The secretome human umbilical cord mesenchymal stromal cells (hUC-MSC-sec) contains several components that might In present study, we revealed treatment hUC-MSC-sec significantly increased proportion activated follicles in mouse ovaries both vitro vivo....
The transcription factor p63 guards genome integrity in the female germline, and its mutations have been reported patients with premature ovarian insufficiency (POI). However, precise contribution of TP63 gene to pathogenesis POI needs be further determined. Here, 1,030 Chinese POI, we identified 6 heterozygous that impaired C-terminal transactivation-inhibitory domain (TID) TAp63α protein resulted tetramer formation constitutive activation mutant proteins. proteins induced cell apoptosis by...
The POU family transcription factor OCT4 is required for maintaining the pluripotency of embryonic stem cells and generating induced pluripotent cells. Although clearly shown to be expressed in some germ cell tumours, its expression human somatic tumours remains controversial. Some studies have that adult cells, cancers and, further, cancer while other failed make such an observation. It thus important ascertain whether tumours. By using RT-PCR sequencing analysis, three pseudogenes, viz....
Abstract: Melatonin, an indolamine produced and secreted predominately by the pineal gland, exhibits a variety of physiological functions, possesses antioxidant antitumor properties. In this study, we have shown that pharmacologic concentration (1 m ) melatonin significantly reduced cell migration invasion T98G U251 glioma cells after 24‐hr treatment inhibited expression matrix metalloproteinase 2 (MMP 2) MMP 9. The inhibition was associated with its reduction intracellular basal free...
Sustaining proliferative signaling is one of the hallmarks cancer. Granulocyte-colony stimulating factor (G-CSF), a hematopoietic growth that controls proliferation and differentiation neural stem cells, may contribute to development glioma. In present study, we evaluated expression G-CSF its receptor (G-CSFR) in various grades glioma samples primary cell culture derived from glioblastoma patient as well different human lines. We showed G-CSFR were widely expressed by samples, The levels not...
Premature ovarian insufficiency (POI) imposes great challenges on women's fertility and lifelong health. POI is highly heterogeneous encompasses occult, biochemical, overt stages. MicroRNAs (miRNAs) are negative regulators of gene expression, whose roles in physiology diseases like cancers neurological disorders have been recognized, but little known about the miRNAs profile functional relevance biochemical (bPOI). In this study, expression mRNAs granulosa cells (GCs) bPOI women was...
Abstract Granulosa cell (GC) is a critical somatic component of ovarian follicles to support oocyte development, while the regulatory role long noncoding RNA (lncRNA) in GCs largely unknown. Here, we identified down-regulated lncRNA ZNF674-AS1 from patients with biochemical premature insufficiency (bPOI), and its expression correlates serum levels clinical reserve indicators. Functional experiments showed that induced by energy stress, regulates proliferation glycolysis GCs, which possibly...
Preserving a high degree of genome integrity and stability in germ cells is utmost importance for reproduction species propagation. However, the regulatory mechanisms maintaining developing primordial (PGCs), which rapid proliferation coupled with global hypertranscription, remain largely unknown. Here, we find that mouse PGCs encounter constitutively frequency transcription-replication conflicts (TRCs), lead to R-loop accumulation impose endogenous replication stress on PGCs. We further...
Abstract Background Premature ovarian insufficiency refers to the loss of function before 40 years age. The etiology is heterogeneous, and genetic factors account for 20–25% cases. However, how transform findings clinical molecular diagnose remains a challenge. To identify potential causative variations POI, next generation sequencing panel with 28 known genes POI was designed, large cohort 500 Chinese Han patients screened directly. Pathogenic evaluation identified variants phenotype...
Oocyte maturation is vital to attain full competence required for fertilization and embryogenesis. NLRP14 preferentially expressed in mammalian oocytes early embryos. Yet, the role molecular mechanism of oocyte embryogenesis are poorly understood, whether deficiency accounts human infertility unknown. Here, we found that maternal loss Nlrp14 resulted sterility with defects embryonic arrest (EEA). ablation compromised due impaired cytoplasmic nuclear maturation. Importantly, revealed...
MCM8 has emerged as a core gene in reproductive aging and is crucial for meiotic homologous recombination repair. It also safeguards genome stability by coordinating the replication stress response during mitosis, but its function mitotic germ cells remains elusive. Here we found that disabling mice resulted proliferation defects of primordial (PGCs) ultimately impaired fertility. We further demonstrated interacted with two known helicases DDX5 DHX9, loss led to R-loop accumulation reducing...