A5047308650- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Pharmacological Receptor Mechanisms and Effects
- Photoreceptor and optogenetics research
- Neuroendocrine regulation and behavior
- Neuroscience of respiration and sleep
- Neurotransmitter Receptor Influence on Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuropeptides and Animal Physiology
- Neuroscience and Neural Engineering
- Biochemical Analysis and Sensing Techniques
- Neurological Disease Mechanisms and Treatments
- Chemical Synthesis and Analysis
- Immune cells in cancer
- Physiological and biochemical adaptations
- Psychedelics and Drug Studies
- Forensic Toxicology and Drug Analysis
- GABA and Rice Research
- PI3K/AKT/mTOR signaling in cancer
- Hemoglobin structure and function
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phytoestrogen effects and research
- Heart Rate Variability and Autonomic Control
China Pharmaceutical University
2024
State Key Laboratory of Natural Medicine
2024
University of North Texas
2010-2020
University of North Texas Health Science Center
2010-2020
University of North Texas System
2014
Rockefeller University
2008
Louisiana State University
2007
Louisiana State University Health Sciences Center Shreveport
2007
Wright State University
1997
Shanghai Institute of Applied Physics
1992
Cancer vaccines show huge potential for cancer prevention and treatment. However, their efficacy remains limited due to weak immunogenicity regarding inefficient stimulation of cytotoxic T lymphocyte (CTL) responses. Inspired by the unique characteristic biological function high-density lipoprotein (HDL), we here develop an HDL-mimicking nanovaccine with commendable lymph-targeted capacity potently elicit antitumor immunity using lipid nanoparticle that is co-loaded specific cytomembrane...
purpose. The ς-1 receptor belongs to a recently discovered family of transmembrane proteins expressed in the central nervous system, including eye, and mediates regulation ion channels. exact function sigma receptors remains be elucidated. purpose this study was investigate effect ligands on calcium homeostasis retinal ganglion cell line (RGC)-5 rat primary RGCs. methods. Calcium imaging used assess agonist (+)-N-allylnormetazocine ((+)-SKF10047) potassium chloride (KCl)-induced influx...
L-type voltage-gated Ca(2+)channels (VGCC) play an important role in dendritic development, neuronal survival, and synaptic plasticity. Recent studies have demonstrated that the gonadal steroid estrogen rapidly induces Ca(2+) influx hippocampal neurons, which is required for neuroprotection potentiation of LTP. The mechanism by this not clearly understood. We show electrophysiological extremely low concentrations estrogens acutely potentiate VGCC slices, HEK-293 cells transfected with VGCC,...
Background and Purpose Cognitive deficits in patients with A lzheimer's disease, P arkinson's traumatic brain injury stroke often involve alterations cholinergic signalling. Currently available therapeutic drugs provide only symptomatic relief. Therefore, novel strategies are needed to retard and/or arrest the progressive loss of memory. Experimental Approach Scopolamine‐induced memory impairment provides a rapid reversible phenotypic screening paradigm for cognition enhancement drug...
Anatomically coupled neurons (17 of 137) and non-coupled (120 137), in near the nucleus tractus solitarius dorsal motor (i.e. solitary complex), were studied by rapid perforated patch recording slices (rat, 150–350 μm thick, postnatal day 0–21) before, during after exposure to hypercapnic acidosis. Anatomical coupling refers intercellular transfer Lucifer Yellow Biocytin into adjoining neurons, presumably via gap junctions [see Dean et al. (1997) Neuroscience, this volume]. Eighty-six per...
We studied the effects of extracellular pH (pHo) on gamma-aminobutyric acid (GABA)-mediated Cl- current in rat hypothalamic neurons and recombinant type-A GABA (GABA(A)) receptors stably expressed human embryonic kidney cells (HEK 293), using whole cell outside-out patch-clamp recordings. In alpha3beta2gamma2s receptors, acidic decreased, whereas alkaline increased response to a reversible concentration-dependent manner. Acidification shifted concentration-response curve right, significantly...
Abstract Acid-sensing ion channels (ASICs) evolved to sense changes in extracellular acidity with the divalent cation calcium (Ca 2+ ) as an allosteric modulator and channel blocker. The channel-blocking activity is most apparent ASIC3, removing Ca results opening, site’s location remaining unresolved. Here we show that a ring of rat ASIC3 (rASIC3) glutamates (Glu435), located above gate, modulates proton sensitivity contributes formation elusive block site. Mutation this residue glycine,...
Dye (Lucifer Yellow) and tracer (Biocytin) coupling, referred to collectively as anatomical were identified in 20% of the solitary complex neurons tested medullary tissue slices (120–350 μm) prepared from rat, postnatal day 1–18, using a modified amphotericin B-perforated patch recording technique. Ten per cent sampled nuclei outside anatomically coupled. Fifty-eight coupled exhibited electrotonic postsynaptic potential-like activity, which had peak-to-peak amplitudes ≤7 mV, with same...
Abstract Progesterone (P4) exerts robust cytoprotection in brain slice cultures (containing both neurons and glia), yet such protection is not as evident neuron-enriched cultures, suggesting that glia may play an indispensable role P4's neuroprotection. We previously reported a membrane-associated P4 receptor, receptor membrane component 1, mediates P4-induced brain-derived neurotrophic factor (BDNF) release from glia. Here, we sought to determine whether are required for neuroprotection...
Extracellular pH regulates glycine receptors through an unknown mechanism. Here we demonstrate that acidic remarkably inhibited glycine-activated whole-cell currents in recombinant α1 and α1β transiently expressed human embryonic kidney 293 cells. The proton effect was voltage-independent pharmacologically competed with receptor agonist antagonist strychnine. Using site-directed mutagenesis, have identified N-terminal domain is essential for proton-induced inhibition of current. In homomers,...
We previously reported on the synthesis of substituted phenyl-4-hydroxy-1-piperidyl indole analogues with nanomolar affinity at D2 dopamine receptors, ranging from 10- to 100-fold selective for compared D3 receptor subtype. More recently, we evaluated a panel aripiprazole analogues, identifying several that also exhibit vs binding selectivity. These studies further characterize intrinsic efficacy compound greatest selectivity each chemical class,...
Little is known regarding the mechanism(s) by which glycine receptors are endocytosed. Here we examined endocytosis of homomeric alpha1 expressed in HEK 293 cells using immunofluorescence/confocal microscopy and whole-cell patch-clamp recordings. Our studies demonstrate that constitutive blocked dominant negative dynamin construct K44A intracellular dialysis with peptide P4, a dynamin/amphiphysin-disrupting peptide, increased glycine-gated chloride currents. To examine whether receptor could...
A cell membrane-derived vesicle (MV) that has cell-mimicking features with characteristic functionalities holds vast appeal for biomimetic nanomedicine and drug delivery but suffers from a major limitation of innate fragility poor stability. Herein, we report lipid-anchoring strategy stabilizing MV enhanced delivery. An array amphiphilic mono-acyl phosphatidylcholines (MPCs) specific hydrophobic moieties are synthesized readily engineered on based their commendable aqueous solubility...