A5082581017- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Carbohydrate Chemistry and Synthesis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Nitric Oxide and Endothelin Effects
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Escherichia coli research studies
- Advanced Nanomaterials in Catalysis
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Viral Infections and Outbreaks Research
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Enzyme Structure and Function
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- Immune Cell Function and Interaction
- Proteoglycans and glycosaminoglycans research
- COVID-19 Clinical Research Studies
- Natural product bioactivities and synthesis
Université Grenoble Alpes
2015-2024
Institut de Biologie Structurale
2015-2024
Centre National de la Recherche Scientifique
2014-2024
CEA Grenoble
2014-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2014-2024
Institut Universitaire de France
2011-2024
Lyon College
2024
Goethe University Frankfurt
2024
Kennesaw State University
2024
Université Claude Bernard Lyon 1
2024
The efficient spread of SARS-CoV-2 resulted in a unique pandemic modern history. Despite early identification ACE2 as the receptor for viral spike protein, much remains to be understood about molecular events behind dissemination. We evaluated contribution C-type lectin receptors (CLRS) antigen-presenting cells, widely present respiratory mucosa and lung tissue. DC-SIGN, L-SIGN, Langerin MGL bind diverse glycans using multiple interaction areas. Using pseudovirus cells derived from monocytes...
The hepatitis C virus (HCV) genome codes for highly mannosylated envelope proteins, which are naturally retained in the endoplasmic reticulum. We found that HCV glycoprotein E2 binds dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and related liver endothelial cell lectin L-SIGN through high-mannose N-glycans. Competing ligands such as mannan an antibody directed against carbohydrate recognition domains (CRD) abrogated binding. While no interaction...
Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated combining different carbohydrate synthesis approaches including automated assembly, solution-phase synthesis, chemoenzymatic methods. The current library more than...
Abstract Reversibly switchable fluorescent proteins (RSFPs) serve as markers in advanced fluorescence imaging. Photoswitching from a non-fluorescent off - state to on involves trans -to- cis chromophore isomerization and proton transfer. Whereas excited-state events the ps timescale have been structurally characterized, conformational changes slower timescales remain elusive. Here we describe off-to-on photoswitching mechanism RSFP rsEGFP2 by using combination of time-resolved serial...
The C-type lectin receptor DC-SIGN has been highlighted as the coreceptor for spike protein of SARS-CoV-2 virus. A multivalent glycomimetic ligand, Polyman26, found to inhibit DC-SIGN-dependent trans-infection SARS-CoV-2. molecular details underlying avidity generation in such systems remain poorly characterized. In an effort dissect contribution known effects - chelation, clustering, and statistical rebinding we studied a series dendrimer constructs related Polyman26 with rod core...
A heterodimer of prenylated Rac1 and Rho GDP dissociation inhibitor was purified found to be competent in NADPH oxidase activation. Small angle neutron scattering experiments confirmed a 1:1 stoichiometry. The crystal structure the Rac1-RhoGDI complex determined at 2.7 resolution. In this which is bound GDP, switch I region conformation whereas II resembles that GTP-bound GTPase. Two types interaction between RhoGTPases RhoGDI were investigated. lipid-protein geranylgeranyl moiety resulted...
HIV infection is pandemic in humans and responsible for millions of deaths every year. The discovery new cellular targets that can be used to prevent the process represents a opportunity developing more effective antiviral drugs. In this context, dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN), lectin expressed at surface immature cells involved initial stages infection, promising therapeutic target. Herein we show ability tetravalent dendron containing four copies linear...
DC-SIGN is a C-type lectin receptor of dendritic cells and involved in the early stages numerous infectious diseases. organized into tetramer enabling multivalent interaction with pathogens. Once formed, DC-SIGN-pathogen complex can be internalized compartments increasing acidity. We have studied pH dependence oligomerization state conformation entire extracellular domain neck region. present evidence for equilibrium between monomeric tetrameric states domain, which exhibits marked respect...
Celastrol is one of several bioactive compounds extracted from the medicinal plant Tripterygium wilfordii. used to treat inflammatory conditions, and shows benefits in models neurodegenerative disease, cancer arthritis, although its mechanism action incompletely understood.
The development of compounds with strong affinity for the receptor DC-SIGN is a topic remarkable interest due to role that this lectin plays in several pathogen infection processes and modulation immune response. recognizes mannosylated fucosylated oligosaccharides multivalent manner. Therefore, carbohydrate systems are required interact an efficient manner compete natural ligands. We have previously demonstrated linear pseudodi- pseudotrisaccharides adequate ligands DC-SIGN. In work, we...
Rational design of DC-SIGN antagonists combines a selective glycomimetic ligand with trivalent dendrons spaced by rigid core and allows to exploit multiple multivalency effects.
In genital mucosa, different fates are described for HIV according to the subtype of dendritic cells (DCs) involved in its recognition. This notably depends on C-type lectin receptor, langerin or DC-SIGN, gp120 interaction. Langerin blocks transmission by internalization specific organelles Langerhans cells. On contrary, DC-SIGN enhances trans-infection T lymphocytes. Thus, approaches aiming inhibit without blocking langerin, represent attractive anti-HIV strategies. We previously...
Langerin is a C-type lectin present on Langerhans cells that mediates capture of pathogens in carbohydrate-dependent manner, leading to subsequent internalization and elimination the cellular organelles called Birbeck granules. This mechanism mediated by langerin was shown constitute natural barrier for HIV-1 particle transmission. Besides interacting specifically with high mannose fucosylated neutral carbohydrate structures, has ability bind sulfated ligands as 6-sulfated galactosides...
At the surface of dendritic cells, C-type lectin receptors (CLRs) allow recognition carbohydrate-based PAMPS or DAMPS (pathogen- danger-associated molecular patterns, respectively) and promote immune response regulation. However, some CLRs are hijacked by viral bacterial pathogens. Thus, design ligands able to target specifically one CLR, either modulate an inhibit a given infection mechanism, has great potential value in therapeutic design. A case study is selective blocking DC-SIGN,...
Due to their ability recognize carbohydrate structures, lectins emerged as potential receptors for bacterial lipopolysaccharides (LPS). Despite growing interest in investigating the association between host receptor and exogenous glycan ligands, molecular mechanisms underlying recognition by human are still not fully understood. We contributed fill this gap unveiling basis of interaction lipooligosaccharide Escherichia coli dendritic cell-specific intracellular adhesion molecules (ICAM)-3...
The NAD(P)H:flavin oxidoreductase from Escherichia coli, Fre, is a monomer of 26.2 kDa that catalyzes the reduction free flavins by NADPH or NADH. Overexpression in E. coli now allows preparation large amounts pure protein. Structural requirements for recognition as substrates and not cofactors were studied steady-state kinetics with variety flavin analogs. entire isoalloxazine ring was found to be essential part molecule interaction polypeptide chain. Methyl groups at C-7 C-8 N-3 riboflavin...
DC‐SIGN (dendritic cell‐specific ICAM‐3 grabbing non‐integrin) is a C‐type lectin receptor of dendritic cells and involved in the initial steps numerous infectious diseases. Surface plasmon resonance has been used to study affinity glycodendritic polymer with 32 mannoses, DC‐SIGN. This glycodendrimer binds surfaces submicromolar range. binding depends on clustered organization mimicking its natural as microdomain plasma membrane. Moreover, this compound inhibits HIV glycoprotein gp120 an IC...