A5005781724- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Protein Structure and Dynamics
- Neuroscience and Neuropharmacology Research
- Molecular spectroscopy and chirality
- Advanced NMR Techniques and Applications
- Spectroscopy and Quantum Chemical Studies
- Retinal Development and Disorders
- Force Microscopy Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Memory and Neural Mechanisms
- Sphingolipid Metabolism and Signaling
- Nanopore and Nanochannel Transport Studies
- NMR spectroscopy and applications
- Antimicrobial Peptides and Activities
- Neural dynamics and brain function
- Surfactants and Colloidal Systems
- Corneal surgery and disorders
- Atomic and Subatomic Physics Research
- Ocular Surface and Contact Lens
- Advanced Fluorescence Microscopy Techniques
- Corneal Surgery and Treatments
- Solid-state spectroscopy and crystallography
- Spatial Cognition and Navigation
University of Arizona
2015-2024
Eastern Virginia Medical School
2023
Applied Mathematics (United States)
2022-2023
Arizona State University
2020-2021
Colorado School of Mines
2015-2017
University of Indianapolis
2014-2015
Indiana University – Purdue University Indianapolis
2014-2015
Villanova University
1990-2011
University of Freiburg
2010
Ludwig-Maximilians-Universität München
2008
Significance Cholesterol regulates critical cell functions, including lysis, viral budding, and antibiotic resistance, by modifying the bending rigidity of membranes; i.e., ability membranes to bend or withstand mechanical stresses. A molecular-level understanding these functions requires knowledge how cholesterol modifies membrane mechanics over relevant length time scales. Currently, it is widely accepted that has no effect on properties unsaturated lipid membranes, implying viruses, for...
The digital revolution has dramatically increased the ability of individuals and corporations to appropriate profit from cultural knowledge indigenous peoples, which is largely unprotected by existing intellectual property law. In response, legal scholars, anthropologists, native activists now propose new regimes designed defend cultures radically expanding notion copyright. Unfortunately, these proposals are often informed romantic assumptions that ignore broader crisis already imperiled...
ADVERTISEMENT RETURN TO ISSUEPREVArticleInfluence of cholesterol on the polar region phosphatidylcholine and phosphatidylethanolamine bilayersMichael F. Brown Joachim SeeligCite this: Biochemistry 1978, 17, 2, 381–384Publication Date (Print):January 24, 1978Publication History Published online1 May 2002Published inissue 24 January 1978https://pubs.acs.org/doi/10.1021/bi00595a029https://doi.org/10.1021/bi00595a029research-articleACS PublicationsRequest reuse permissionsArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLipid headgroup and acyl chain composition modulate the MI-MII equilibrium of rhodopsin in recombinant membranesNicholas J. Gibson Michael F. BrownCite this: Biochemistry 1993, 32, 9, 2438–2454Publication Date (Print):March 1993Publication History Published online1 May 2002Published inissue 9 March 1993https://pubs.acs.org/doi/10.1021/bi00060a040https://doi.org/10.1021/bi00060a040research-articleACS PublicationsRequest reuse permissionsArticle...
Membrane biochemists are becoming increasingly aware of the role lipid-protein interactions in diverse cellular functions. This review describes how conformational changes membrane proteins, involving folding, stability, and shape transitions, potentially involve elastic remodeling lipid bilayer. Evidence suggests that lipids affect proteins through a relatively long-range nature, extending beyond single annulus next-neighbor boundary lipids. It is assumed distance scale forces large...
Activation of the G protein-coupled receptor (GPCR) rhodopsin is initiated by light-induced isomerization retinal ligand, which triggers 2 protonation switches in conformational transition to active state Meta II. The first switch involves disruption an interhelical salt bridge internal proton transfer from protonated Schiff base (PSB) its counterion, Glu-113, transmembrane domain. second consists uptake a solvent Glu-134 conserved E(D)RY motif at cytoplasmic terminus helix 3, leading...
Natural abundance 13C spin-lattice (T1) relaxation time measurements are reported for unilamellar vesicles of 1,2-dipalmitoylphosphatidylcholine (1,2-dipalmitoyl-sn-glycero-3-phosphocholine), in the liquid crystalline phase, at magnetic field strengths 1.40, 1.87, 2.35, 4.23, 7.05, 8.45, and 11.7 tesla (resonance frequencies 15.0, 20.0, 25.1, 45.3, 75.5, 90.5, 126 MHz, respectively), results compared to previous 2H T1 studies multilamellar dispersions. For both studies, a dramatic frequency...
Sphingomyelin is a lipid that abundant in the nervous systems of mammals, where it associated with putative microdomains cellular membranes and undergoes alterations due to aging or neurodegeneration. We investigated effect varying concentration cholesterol binary ternary mixtures N-palmitoylsphingomyelin (PSM) 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) using deuterium nuclear magnetic resonance (2H NMR) spectroscopy both macroscopically aligned unoriented multilamellar...
Investigation of the deuterium (2H) nuclear magnetic resonance (NMR) relaxation rates lipid bilayers containing cholesterol can yield new insights regarding its role in membrane function and dynamics. Spin-lattice (R1Z) quadrupolar order (R1Q) H2 NMR were measured at 46.1 76.8 MHz for macroscopically oriented 1,2-diperdeuteriomyristoyl-sn-glycero-3-phosphocholine (DMPC-d54) (1/1 molar ratio) liquid-ordered phase 40 °C. The data various segmental positions along DMPC-d54 acyl chain...
In this work we have calculated spin-lattice (T1) relaxation time expressions for the homo- and heteronuclear dipolar of lipid bilayers, in addition to Overhauser enhancement (NOE), using correlation functions derived previously. The results can be applied analysis 1H 13C T1 times bilayers 13C–1H NOE. Three different models segmental fluctuations membraneous molecules been considered: (i) a simple diffusion-type model local motions; (ii) noncollective which relatively slow bilayer are...