A5013027895- Enzyme Structure and Function
- Hemoglobin structure and function
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Hemoglobinopathies and Related Disorders
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Bacteriophages and microbial interactions
- Heme Oxygenase-1 and Carbon Monoxide
- Biochemical and Molecular Research
- Neonatal Health and Biochemistry
- Monoclonal and Polyclonal Antibodies Research
- Antibiotic Resistance in Bacteria
- Chemical Synthesis and Analysis
- Enzyme Production and Characterization
- Photoreceptor and optogenetics research
- Escherichia coli research studies
- Advanced Thermodynamics and Statistical Mechanics
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Drug Transport and Resistance Mechanisms
- Mass Spectrometry Techniques and Applications
- RNA modifications and cancer
- Physiological and biochemical adaptations
Yokohama City University
2015-2024
Fred Hutch Cancer Center
2024
Griffith University
2024
RIKEN
2013
Tsurumi University
2011
Sequenom (United States)
2009
University of Warwick
2005-2009
Tokyo Institute of Technology
2009
Japan Science and Technology Agency
2007
Nara Institute of Science and Technology
2007
Around 250 million people are infected with hepatitis B virus (HBV) worldwide1, and 15 may also carry the satellite D (HDV), which confers even greater risk of severe liver disease2. The HBV receptor has been identified as sodium taurocholate co-transporting polypeptide (NTCP), interacts directly first 48 amino acid residues N-myristoylated N-terminal preS1 domain viral large protein3. Despite pressing need for therapeutic agents to counter HBV, structure NTCP remains unsolved. This...
Specific protein-ligand interactions are critical for cellular function, and most proteins select their partners with sharp discrimination. However, the oligopeptide-binding protein of Salmonella typhimurium (OppA) binds peptides two to five amino acid residues without regard sequence. The crystal structure OppA reveals a three-domain organization, unlike other periplasmic binding proteins. In OppA-peptide complexes, ligands completely enclosed in interior, mode that normally imposes tight...
The acquisition of iron is essential for the survival pathogenic bacteria, which have consequently evolved a wide variety uptake systems to extract and heme from host proteins such as hemoglobin. Hemoglobin protease (Hbp) was discovered factor involved in symbiosis Escherichia coli Bacteroides fragilis, cause intra-abdominal abscesses. Released E. coli, this serine autotransporter degrades hemoglobin delivers both bacterial species. crystal structure complete passenger domain Hbp (110 kDa)...
Abstract: Pharmacophore modeling is a successful yet very diverse subfield of computer-aided drug design. The concept the pharmacophore has been widely applied to rational design novel drugs. In this paper, we review computational implementation and its common usage in discovery process. Pharmacophores can be used represent identify molecules on 2D or 3D level by schematically depicting key elements molecular recognition. most application pharmacophores virtual screening, different...
Significance In this study, we have designed and experimentally validated, to our knowledge, the first perfectly symmetrical β-propeller protein. Our results provide insight not only into protein evolution through duplication events, but also methods for creating designer proteins that self-assemble according simple arithmetical rules. Such may very wide uses in bionanotechnology. Furthermore design approach is both rapid applicable many different templates. novel propeller consists of six...
Neutrophil granulocytes play key roles in innate immunity and shaping adaptive immune responses. They are attracted by chemokines to sites of infection tissue damage, where they kill phagocytose bacteria. The chemokine CXCL8 (also known as interleukin-8, abbreviated IL-8) its G-protein-coupled receptors CXCR1 CXCR2 crucial elements this process, also the development many cancers. These GPCRs have therefore been target drug campaigns structural studies. Here, we solve structure complexed with...
Of two closely related species of geese, one, the greylag goose, lives in Indian plains all year round, while other, bar-headed at Tibetan lakes and migrates across Himalayas to winter India. Another species, Andean High Andes round. Possession a Hb with high oxygen affinity helps adapt geese altitudes. The amino acid sequences differ by four substitutions, which only one is unique among bird sequences: Pro-119 alpha (H2)----Ala. Perutz proposed that two-carbon gap left this substitution 1...
Summary Many virulence factors secreted by pathogenic Gram‐negative bacteria belong to the autotransporter (AT) family. ATs consist of a passenger domain, which is actual moiety, and β‐domain that facilitates transfer domain across outer membrane. Here, we analysed folding translocation AT passenger, using Escherichia coli haemoglobin protease (Hbp) as model protein. Dual cysteine mutagenesis, instigated unique crystal structure Hbp resulted in intramolecular disulphide bond formation...
Human noroviruses are genetically and antigenically highly divergent. Monoclonal antibodies raised in mice against one kind of norovirus virus-like particle (VLP), however, were found to have broad recognition. In this study, we present the crystal structure antigen-binding fragment (Fab) for these broadly reactive monoclonal antibodies, 5B18, complex with capsid-protruding domain from a genogroup II genotype 10 (GII.10) at 3.3-Å resolution and, also, cryo-electron microscopy GII.10 VLP...
Significance Optogenetics is a rapidly growing field in which light used to control biological systems. We show that Oscillatoria acuminata photoactivated adenylate cyclase (OaPAC) protein produces the fundamental second messenger cyclic-AMP (cAMP) response blue light, stable and functional different mammalian cell types, can be trigger events by raising cAMP level. OaPAC consists of catalytic domain controlled photosensitive using flavin (BLUF) domain. have solved crystal structure how...
During the adaptive evolution of a particular trait, some selectively fixed mutations may be directly causative and others purely compensatory. The relative contribution these two classes mutation to phenotypic depends on form prevalence mutational pleiotropy. To investigate nature substitutions their pleiotropic effects, we used protein engineering approach characterize molecular basis hemoglobin (Hb) adaptation in high-flying bar-headed goose (Anser indicus), hypoxia-tolerant species...
Significance Light-driven rhodopsin proteins pump ions across cell membranes. They have applications in optogenetics and can potentially be used to develop solar energy–harvesting devices. A detailed understanding of dynamics functions may therefore assist research medicine, health, clean energy. This time-resolved crystallography study carried out with X-ray free-electron lasers reveals chloride ion–pumping (ClR) within 100 ps light activation. It shows the dissociation Cl − from Schiff...
Translation elongation factor P (EF-P) stimulates ribosomal peptidyltransferase activity. EF-P is conserved in bacteria and essential for cell viability. Eukarya Archaea have an homologue, eukaryotic initiation 5A (eIF-5A). In the present study, we determined crystal structure of from Thermus thermophilus HB8 at a 1.65-Å resolution. consists three β-barrel domains (I, II, III), whereas eIF-5A has only two (N C domains). Domain I topologically same as N domain eIF-5A. On other hand, II III...
Abstract Association and dissociation rate constants for O2, CO, methyl isocyanide binding to native distal pocket mutants of R state human hemoglobin were measured using ligand displacement partial photolysis techniques. Individual the alpha beta subunits resolved by comparisons between kinetic behavior mutant proteins. His-E7 was replaced with Gly Gln in both Phe alone. In separate experiments Val-E11 Ala, Leu, Ile each globin chain. The parameters describing are sensitive size polarity...