A5100435890- Carbohydrate Chemistry and Synthesis
- Receptor Mechanisms and Signaling
- Glycosylation and Glycoproteins Research
- Cannabis and Cannabinoid Research
- Hormonal and reproductive studies
- Escherichia coli research studies
- Neuroscience and Neuropharmacology Research
- Hormonal Regulation and Hypertension
- Ion channel regulation and function
- RNA Research and Splicing
- Diabetes Treatment and Management
- Cancer-related gene regulation
- Microbial Natural Products and Biosynthesis
- Metabolism, Diabetes, and Cancer
- Peptidase Inhibition and Analysis
- Eicosanoids and Hypertension Pharmacology
- Diet, Metabolism, and Disease
- Legume Nitrogen Fixing Symbiosis
- Neuropeptides and Animal Physiology
- Adenosine and Purinergic Signaling
- Sexual Differentiation and Disorders
- Menopause: Health Impacts and Treatments
- RNA modifications and cancer
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
Hebei North University
2024
International Potato Center
2024
Nanjing University
2024
Nanjing General Hospital of Nanjing Military Command
2024
University of Science and Technology of China
2012-2024
Zhejiang Sci-Tech University
2024
Guangxi University of Chinese Medicine
2024
Southern University of Science and Technology
2020-2024
China Resources (China)
2024
Shandong Academy of Sciences
2023-2024
Endogenous cannabinoids acting at CB(1) receptors stimulate appetite, and antagonists show promise in the treatment of obesity. (-/-) mice are resistant to diet-induced obesity even though their caloric intake is similar that wild-type mice, suggesting endocannabinoids also regulate fat metabolism. Here, we investigated possible role regulation hepatic lipogenesis. Activation increases gene expression lipogenic transcription factor SREBP-1c its targets acetyl-CoA carboxylase-1 fatty acid...
Endogenous cannabinoids acting at CB1 receptors stimulate appetite, and antagonists show promise in the treatment of obesity. CB1–/– mice are resistant to diet-induced obesity even though their caloric intake is similar that wild-type mice, suggesting endocannabinoids also regulate fat metabolism. Here, we investigated possible role regulation hepatic lipogenesis. Activation increases gene expression lipogenic transcription factor SREBP-1c its targets acetyl-CoA carboxylase-1 fatty acid...
The endocannabinoid arachidonoyl ethanolamine (anandamide) is a lipid transmitter synthesized and released “on demand” by neurons in the brain. Anandamide also generated macrophages where its endotoxin (LPS)-induced synthesis has been implicated hypotension of septic shock advanced liver cirrhosis. can be from membrane precursor, N -arachidonoyl phosphatidylethanolamine (NAPE) through cleavage phospholipase D (NAPE–PLD). Here we document biosynthetic pathway for anandamide mouse brain...
Background: The sodium-glucose co-transporter-2 (SGLT-2) inhibitor dapagliflozin improves cardiovascular outcomes in patients with type 2 diabetes a manner that is partially independent of its hypoglycemic effect. These observations suggest it may exert cardioprotective effect by another mechanism. This study explored the effects on myocardial ischemia/reperfusion injury mouse model. Materials and Methods: For vivo I/R studies, mice received 40 mg/kg/d dapagliflozin, starting 7 days before...
Cannabinoids and ethanol can activate the same reward pathways, which could suggest endocannabinoid involvement in rewarding effects of ethanol. The high preference young (6-10 weeks) C57BL6J mice is reduced by cannabinoid receptor 1 (CB1) antagonist SR141716A to levels observed their CB1 knockout littermates or old (26-48 wild-type mice, both unaffected SR141716A. Similarly, inhibits food intake food-restricted young, but not old, mice. There are no age-dependent differences tissue...
Upon interaction with anionic phospholipids, particularly mitochondria-specific cardiolipin (CL), cytochrome c (cyt c) loses its tertiary structure and peroxidase activity dramatically increases. CL-induced of cyt has been found to be important for selective CL oxidation in cells undergoing programmed death. During apoptosis, the fraction CL-bound markedly increase, suggesting that may act as a switch regulate c's mitochondrial functions. Using cyclic voltammetry equilibrium redox...
Abstract Formylpeptide receptors (FPRs) as G protein-coupled (GPCRs) can recognize formylpeptides derived from pathogens or host cells to function in defense and cell clearance. In addition, FPRs, especially FPR2, also other ligands with a large chemical diversity generated at different stages of inflammation either promote resolve order maintain balanced inflammatory response. The mechanism underlying promiscuous ligand recognition activation FPRs is not clear. Here we report cryo-EM...
Staphylococcus aureus, a Gram-positive bacterium causes number of devastating human diseases, such as infective endocarditis, osteomyelitis, septic arthritis and sepsis. S. aureus SraP, surface-exposed serine-rich repeat glycoprotein (SRRP), is required for the pathogenesis endocarditis via its ligand-binding region (BR) adhering to platelets. It remains unclear how SraP interacts with host. Here we report 2.05 Å crystal structure BR revealing an extended rod-like architecture four discrete...
Human steroid 5α-reductase 2 (SRD5A2) is an integral membrane enzyme in metabolism and catalyzes the reduction of testosterone to dihydrotestosterone. Mutations SRD5A2 gene have been linked deficiency prostate cancer. Finasteride dutasteride, as inhibitors, are widely used antiandrogen drugs for benign hyperplasia. The molecular mechanisms underlying catalysis inhibition other eukaryotic reductases remain elusive due a lack structural information. Here, we report crystal structure human at...
Abstract The formylpeptide receptors (FPRs) mediate pattern recognition of formylated peptides derived from invading pathogens or mitochondria dead host cells. They can also sense other structurally distinct native and even lipid mediators to either promote resolve inflammation. Pharmacological targeting FPRs represents a novel therapeutic approach in treating inflammatory diseases. However, the molecular mechanisms underlying FPR ligand are elusive. We report cryo-EM structures G i -coupled...
ABSTRACT Capsular polysaccharide (CPS) is a major virulence factor in Streptococcus pneumoniae . CPS gene clusters of S. types 4, 6B, 8, and 18C were sequenced compared with those 1, 2, 14, 19F, 19A, 23F, 33F. All have the same four genes at 5′ end, encoding proteins thought to be involved regulation export. Sequences these can divided into two classes, evidence recombination between them was observed. Next transferase for first step synthesis CPS. The predicted amino acid sequences sugar...
Summary Aims Stroke is a major cause of mortality and disability, especially for postmenopausal women. In view the protective action estrogen, hormone therapy remains only effective way to limit this risk. The objective study was investigate efficiency underlying mechanisms estrogen neuroprotection. Methods Subcutaneous injection 17β‐estradiol in rats after ovariectomy ( OVX ) used manipulate level explore effects cerebral ischemic damage both vivo vitro. Silent mating type information...
Hemp ( Cannabis sativa L.) seed is emerging as a novel source of plant protein owing to its rich content and reasonable nutritional structure. In the current study, storage proteins hemp were extracted using different methods. The modified Osborne method yielded maximum extraction proteins, while degreasing had little effect on (HSP) extraction. Protein identification results revealed that 11S globulin (edestin) was most abundant in seed, molecular weights two subunits this ~35 20 kDa,...
Abstract Myosin VI is the only molecular motor that moves towards minus end along actin filaments. Numerous cellular processes require myosin and tight regulations of motor’s activity. Defects in activity are known to cause genetic diseases such as deafness cardiomyopathy. However, mechanisms underlying regulation remain elusive. Here, we determined high-resolution cryo-electron microscopic structure its autoinhibited state. Our reveals adopts a compact, monomeric conformation via extensive...