A5089258049- Cardiomyopathy and Myosin Studies
- Cellular Mechanics and Interactions
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Protein Structure and Dynamics
- Muscle Physiology and Disorders
- Nuclear Structure and Function
- Enzyme Structure and Function
Southern University of Science and Technology
2022-2024
Harbin Institute of Technology
2022
Abstract Myosin VI is the only molecular motor that moves towards minus end along actin filaments. Numerous cellular processes require myosin and tight regulations of motor’s activity. Defects in activity are known to cause genetic diseases such as deafness cardiomyopathy. However, mechanisms underlying regulation remain elusive. Here, we determined high-resolution cryo-electron microscopic structure its autoinhibited state. Our reveals adopts a compact, monomeric conformation via extensive...
As the prototype of unconventional myosin motor family, Va (MyoVa) transport cellular cargos along actin filaments in diverse processes. The off-duty MyoVa adopts a closed and autoinhibited state, which can be relieved by cargo binding. molecular mechanisms governing autoinhibition activation remain unclear. Here, we report cryo-electron microscopy structure two full-length, heavy chains complex with 12 calmodulin light at 4.78-Å resolution. triangular multiple intra- interpolypeptide chain...
Myo2, a yeast class V myosin, transports broad range of organelles and plays important roles in various cellular processes, including cell division budding yeast. Despite the fact that several structures Myo2/cargo adaptor complexes have been determined, understanding versatile cargo-binding modes Myo2 is still very limited, given large number cargo adaptors identified for Myo2. Here, we used ColabFold, an AlphaFold2-powered easy-to-use tool, to predict complex Myo2-GTD its adaptors. After...