A5061744695- Tryptophan and brain disorders
- Drug-Induced Hepatotoxicity and Protection
- Drug Transport and Resistance Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Diet and metabolism studies
- Advanced Proteomics Techniques and Applications
- Pharmacogenetics and Drug Metabolism
- Liver Disease Diagnosis and Treatment
- Treatment of Major Depression
- Mass Spectrometry Techniques and Applications
- Mental Health Research Topics
- Health disparities and outcomes
- Pharmacological Effects and Toxicity Studies
- Metabolism and Genetic Disorders
- Infant Nutrition and Health
- Poisoning and overdose treatments
- Colorectal Cancer Screening and Detection
- Enzyme Structure and Function
- Stress Responses and Cortisol
- Food Security and Health in Diverse Populations
- Liver Disease and Transplantation
- Pharmacological Effects of Natural Compounds
- Protein Structure and Dynamics
- Functional Brain Connectivity Studies
Arkansas Biosciences Institute
2020-2025
Arkansas State University
2020-2025
GGZ inGeest
2024
Vrije Universiteit Amsterdam
2024
Amsterdam University Medical Centers
2024
Emory University
2024
University of Missouri–Kansas City
2024
University of Arkansas for Medical Sciences
2013-2022
University of Arkansas at Little Rock
2022
Washington Center
2022
Abstract Introduction Increasing evidence suggests a role for the gut microbiome in central nervous system disorders and specific gut‐brain axis neurodegeneration. Bile acids (BAs), products of cholesterol metabolism clearance, are produced liver further metabolized by bacteria. They have major regulatory signaling functions seem dysregulated Alzheimer's disease (AD). Methods Serum levels 15 primary secondary BAs their conjugated forms were measured 1464 subjects including 370 cognitively...
Increasing evidence suggests Alzheimer's disease (AD) pathophysiology is influenced by primary and secondary bile acids, the end product of cholesterol metabolism. We analyze 2,114 post-mortem brain transcriptomes identify genes in alternative acid synthesis pathway to be expressed brain. A targeted metabolomic analysis acids measured from samples 111 individuals supports these results. Our metabolic network that taurine transport, synthesis, metabolism differ AD cognitively normal...
Abstract This study investigated the relationship between gut microbiota and neuropsychiatric disorders (NPDs), specifically anxiety disorder (ANXD) and/or major depressive (MDD), as defined by Diagnostic Statistical Manual of Mental Disorders (DSM)-IV or V criteria. The also examined influence medication use, particularly antidepressants anxiolytics, classified through Anatomical Therapeutic Chemical (ATC) Classification System, on microbiota. Both 16S rRNA gene amplicon sequencing (16S)...
The influx of genomic sequence information has led to the concept structural proteomics, determination protein structures on a genome-wide scale. Here we describe an approach proteomics small proteins using NMR spectroscopy. Over 500 from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% these found be readily amenable structure determination. sample preparation centralized in one facility, distributive used...
<h3>Objective</h3> To investigate the association of triglyceride (TG) principal component scores with Alzheimer disease (AD) and amyloid, tau, neurodegeneration, cerebrovascular (A/T/N/V) biomarkers for AD. <h3>Methods</h3> Serum levels 84 TG species were measured untargeted lipid profiling 689 participants from Alzheimer9s Disease Neuroimaging Initiative cohort, including 190 cognitively normal older adults (CN), 339 mild cognitive impairment (MCI), 160 Principal analysis factor rotation...
Abstract Selective serotonin reuptake inhibitors (SSRIs) are the first-line treatment for major depressive disorder (MDD), yet their mechanisms of action not fully understood and therapeutic benefit varies among individuals. We used a targeted metabolomics approach utilizing panel 180 metabolites to gain insights into response citalopram/escitalopram. Plasma samples from 136 participants with MDD enrolled Mayo Pharmacogenomics Research Network Antidepressant Medication Pharmacogenomic Study...
Abstract It is unknown whether indoles, metabolites of tryptophan that are derived entirely from bacterial metabolism in the gut, associated with symptoms depression and anxiety. Serum samples (baseline, 12 weeks) were drawn participants (n = 196) randomized to treatment cognitive behavioral therapy (CBT), escitalopram, or duloxetine for major depressive disorder. Baseline indoxyl sulfate abundance was positively correlated severity psychic anxiety total resting state functional connectivity...
The gut microbiome may play a role in the pathogenesis of neuropsychiatric diseases including major depressive disorder (MDD). Bile acids (BAs) are steroid that synthesized liver from cholesterol and further processed by gut-bacterial enzymes, thus requiring both human enzymatic processes their metabolism. BAs participate range important host functions such as lipid transport metabolism, cellular signaling regulation energy homeostasis. have recently been implicated pathophysiology...
Developing biomarkers for detecting acetaminophen (APAP) toxicity has been widely investigated. Recent studies of adults with APAP-induced liver injury have reported human serum microRNA-122 (miR-122) as a novel biomarker injury. The goal this study was to examine extracellular microRNAs (miRNAs) potential APAP in children. Global levels and urine miRNAs were examined three pediatric subgroups: 1) healthy children (n=10), 2) hospitalized receiving therapeutic doses (n=10) 3) overdose (n=8)....
Abstract Metabolomics provides valuable tools for the study of drug effects, unraveling mechanism action and variation in response due to treatment. In this we used electrochemistry-based targeted metabolomics gain insights into mechanisms escitalopram/citalopram focusing on a set 31 metabolites from neurotransmitter-related pathways. Overall, 290 unipolar patients with major depressive disorder were profiled at baseline, after 4 8 weeks The 17-item Hamilton Depression Rating Scale (HRSD 17...
In the United States, mortality rates from pancreatic cancer (PCa) have not changed significantly over past 50 years. This is due, in part, to lack of early detection methods for this particularly aggressive form cancer. The objective study was use highthroughput protein profiling technology identify biomarkers serum proteome resectable PCa. Using surface-enhanced laser desorption/ionization mass spectrometry, profiles were generated sera 49 PCa patients and 54 unaffected individuals after...
Long-chain acylcarnitines have been postulated to be sensitive biomarkers of acetaminophen (APAP)-induced hepatotoxicity in mouse models. In the following study, relationship with other known indicators APAP toxicity was examined children receiving low-dose (therapeutic) and high-dose ('overdose' or toxic ingestion) exposure APAP.The study included three subject groups: group A (therapeutic dose, n = 187); B (healthy controls, 23); C (overdose, 62). Demographic, clinical laboratory data were...
MicroRNAs (miRNAs) that regulate the cytochrome P-450 isoforms involved in acetaminophen (APAP) toxicity were examined HepaRG cells treated with APAP (20 mM). In-vitro studies found protein adducts increased at 1 h, followed by ALT increases 12 and 24 h. CYP1A2, CYP3A4 CYP2E1 mRNA levels decreased, while miRNAs for miR-122-5p, miR-378a-5p, miR-27b-3p 6 h miR-125b-5p Putative miRNA binding sites on 3'UTRs of CYPs identified in-silico. Overexpression miR-122-5p miR-378a-5p suppressed...
Propionic Acid (PPA) is a ubiquitous short chain fatty acid which fermentation product of the enteric microbiome. While PPA has beneficial effects, it also associated with human disorders, including autism spectrum disorders (ASD). We previously demonstrated that modulates mitochondrial dysfunction differentially in subsets lymphoblastoid cell lines (LCLs) derived from patients ASD. Specifically significantly increases function LCLs have at baseline (called AD-A LCLs) as compared to ASD...
Acylcarnitines have important functions in mitochondrial energetics and β-oxidation, been implicated to play a significant role metabolic of the brain. This retrospective study examined whether plasma acylcarnitine profiles can help biochemically distinguish three phenotypic subtypes major depressive disorder (MDD): core depression (CD+), anxious (ANX+), neurovegetative symptoms melancholia (NVSM+). Depressed outpatients (n = 240) from Mayo Clinic Pharmacogenomics Research Network were...
The use of addictive substances remains a major health problem in the U.S. An increase drug overdose and mortality was observed during COVID-19 pandemic, especially underserved populations. This surge also impacted pregnant women with marijuana (THC) opioids. Retrospective study analyzing mother-infant dyads reported Meconium Drug Screen (MDS) results, from January 2018 through April 2023 at reference hospital serving an urban rural area Arkansas. Due to absence universal screening,...
1 A 12 week prospective survey of all patients any age with confirmed or suspected poisoning presenting to six accident and emergency departments in the North East England was performed establish local incidence patterns presentation poisoning. 2 945 episodes involving 852 were recorded representing approximately 1.2% A&E presentations suggesting an annual attendance rate 2.7 per 1000 persons year. 3 Attendance rates varied threefold between hospitals similar males females overall; ages...
High doses of acetaminophen (APAP) result in hepatotoxicity that involves metabolic activation the parent compound, covalent binding reactive intermediate N-acetyl-p-benzoquinone imine (NAPQI) to liver proteins, and depletion hepatic glutathione. Impaired fatty acid β-oxidation has been implicated previous studies APAP-induced hepatotoxicity. To better understand relationships between toxicity APAP toxicity, metabolomic assays for long chain acylcarnitines were examined relationship...
Abstract Early diagnosis of the onset osteoporosis is key to delivery effective therapy. Biochemical markers bone turnover provide a means evaluating skeletal dynamics that complements static measurements BMD by DXA. Conventional clinical turnover, primarily estimation collagen and its breakdown products in blood or urine, lack both sensitivity specificity as reliable diagnostic tool. As result, improved tests are needed augment use principle modality. In this study, serum proteome 58...