A5009773007- Nonlinear Dynamics and Pattern Formation
- Nonlinear Photonic Systems
- Aeolian processes and effects
- RNA and protein synthesis mechanisms
- Ocean Waves and Remote Sensing
- Particle Dynamics in Fluid Flows
- Surface Modification and Superhydrophobicity
- Gene Regulatory Network Analysis
- Bacterial Genetics and Biotechnology
- Fluid Dynamics and Heat Transfer
- Oceanographic and Atmospheric Processes
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Fluid Dynamics and Turbulent Flows
- Advanced Fiber Laser Technologies
- Micro and Nano Robotics
- RNA regulation and disease
- Bioinformatics and Genomic Networks
- Radio Wave Propagation Studies
- Fluid Dynamics Simulations and Interactions
- Coastal and Marine Dynamics
- Protein Structure and Dynamics
- Geological and Geochemical Analysis
- Advanced Thermodynamics and Statistical Mechanics
- Single-cell and spatial transcriptomics
University of O'Higgins
2017-2023
Universidad Bernardo O'Higgins
2021
University of Córdoba
2020
Service de Physique de l'État Condensé
2013-2019
CEA Paris-Saclay
2013-2019
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2013-2019
Centre National de la Recherche Scientifique
2013-2019
Universidad Católica del Norte
2017-2019
University of Chile
2005-2017
Arizona State University
2014
The influx of genomic sequence information has led to the concept structural proteomics, determination protein structures on a genome-wide scale. Here we describe an approach proteomics small proteins using NMR spectroscopy. Over 500 from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% these found be readily amenable structure determination. sample preparation centralized in one facility, distributive used...
Drop impact causes severe surface erosion, dictating many important natural, environmental and engineering processes calling for substantial prevention preservation efforts. Nevertheless, despite extensive studies on the kinematic features of impacting drops over last two decades, dynamic process that leads to drop-impact erosion is still far from clear. Here, we develop a method high-speed stress microscopy, which measures key properties drop responsible i.e., shear pressure distributions...
ABSTRACT The carbon storage regulator A (CsrA) is a protein responsible for the repression of variety stationary-phase genes in bacteria. In this work, we describe nuclear magnetic resonance (NMR)-based structure CsrA dimer and its RNA-binding properties. two identical subunits, each composed five strands, small α-helix flexible C terminus. NMR titration experiments suggest that β1-β2 β3-β4 loops C-terminal helix are important elements RNA binding. Even though loop contains highly conserved...
Wave patterns in the Faraday instability have been studied for decades. Besides rich wave dynamics observed at interface, waves hide elusive flow bulk – streaming which not experimentally. The are responsible a net circulation flow, is reminiscent of convection cells. In this article, we analyse these flows by conducting experiments Faraday-wave set-up using particle image velocimetry. To visualise flows, perform stroboscopic measurements to both generate trajectory maps and probe velocity...
Gravity wave turbulence is studied experimentally in a large basin where irregular waves are generated unidirectionally. The role of the boundary conditions (absorbing or reflecting) and forcing properties investigated. To that purpose, an absorbing sloping beach opposite to wavemaker can be replaced by reflecting vertical wall. We observe field depend strongly on these conditions. Quasi-one dimensional nonlinear propagate before damped whereas more multidirectional observed with In both...
In the present work we extend and analyze scope of our recently proposed stochastic model for transcriptional regulation, which considers an arbitrarily complex cis-regulatory system using only elementary reactions. Previously, determined role cooperativity on intrinsic fluctuations gene expression activating switches, by means master equation formalism computer simulation. This allowed us to distinguish between two cooperative binding mechanisms and, even though mean levels were not...
2′,3′-Cyclic-nucleotide 3′-phosphodiesterase (CNP) is an enzyme abundantly present in the central nervous system of mammals and some vertebrates. In vitro, CNP specifically catalyzes hydrolysis 2′,3′-cyclic nucleotides to produce 2′-nucleotides, but physiologically relevant vivo substrate remains obscure. Here, we report medium resolution NMR structure catalytic domain rat with phosphate bound describe its binding inhibitors. The has a bilobal arrangement two modules, each consisting...
Rho-dependent transcription termination is an essential process for the regulation of bacterial gene expression. Thus far, only two Rho-specific inhibitors have been described, psu protein from satellite bacteriophage P4 and YaeO Escherichia coli. Here, we report solution structure YaeO, first a inhibitor termination. acidic composed N-terminal helix seven-stranded beta sandwich. NMR chemical shift perturbation experiments revealed that binds proximal to primary nucleic acid binding site...
The origin of stochastic fluctuations in gene expression has received considerable attention recently. Fluctuations are particularly pronounced cellular systems because the small copy number species undergoing transitions between discrete chemical states and size biological compartments. In this paper, we propose a model for regulation including several binding sites, considering elementary reactions only. is used to investigate role cooperativity on intrinsic by means master-equation...
Cry11Bb is an insecticidal crystal protein produced by Bacillus thuringiensis subsp. medellin during its stationary phase; this <FONT FACE="Symbol">¶</FONT>-endotoxin active against dipteran insects and has great potential for mosquito borne disease control. Here, we report the first theoretical model of tridimensional structure a Cry11 toxin. The toxin was obtained homology modelling on structures Cry1Aa Cry3Aa toxins. In work give brief description our hypothesize residues that could be...
aIF2 beta is the archaeal homolog of eIF2 beta, a member heterotrimeric complex, implicated in delivery Met-tRNA(i)(Met) to 40S ribosomal subunit. We have determined solution structure intact beta-subunit from Methanobacterium thermoautotrophicum. composed an unfolded N terminus, mixed alpha/beta core domain and C-terminal zinc finger. NMR data shows two folded domains display restricted mobility with respect each other. Analysis gamma docked tRNA allowed identification putative binding site...
We report an experimental study on the transition between a disordered liquid-like state and ordered solid-like one, in collection of magnetically interacting macroscopic grains. A monolayer magnetized particles is vibrated vertically at moderate density. At high excitation disordered, observed. When driving dimensionless acceleration $\Gamma$ quasi-statically reduced, clusters grains grow below critical value $\Gamma_{\rm c}$. These have well defined hexagonal compact structure. If...
We study the propagation of monochromatic surface waves on a turbulent flow liquid metal, when are much less energetic than background flow. Electromagnetic forcing drives quasi-two-dimensional turbulence with strong vertical vorticity. To isolate surface-wave field, we remove deformation induced by using coherent-phase averaging at wave frequency. observe significant increase in wavelength, latter is smaller length scale. This phenomenon has not been reported before and can be explained...
We show and characterize numerically moving breathing pulses in the one-dimensional complex cubic-quintic Ginzburg-Landau equation. This class of stable has not been described before for this prototype envelope equation as it arises near weakly hysteretic onset traveling waves.
In this letter we experimentally demonstrate self-organization of small tracers under the action longitudinal Faraday waves in a narrow container. We observe steady current formation dividing interface cells given by symmetries wave. These streaming currents are rotating each cell and their circulation increases with wave amplitude. This flow drives to form patterns, whose shapes depend on wave's amplitude: from low high amplitudes find dispersed tracers, ring spiral galaxy-like pattern....
We study numerically a prototype equation which arises generically as an envelope for weakly inverted bifurcation associated to traveling waves: The complex quintic Ginzburg–Landau equation. show six different stable localized structures including stationary pulses, moving holes and holes, starting from initial conditions with periodic Neumann boundary conditions.
Certain spatial distributions of water inside partially filled containers can significantly reduce the bounce container. In experiments with to a volume fraction ϕ, we show that rotation offers control and high efficiency in setting such and, consequently, altering markedly. High-speed imaging evidences physics phenomenon reveals rich sequence fluid-dynamics processes, which translate into model captures our overall experimental findings.
The cubic–quintic complex Ginzburg–Landau is the amplitude equation for systems in vicinity of an oscillatory sub-critical bifurcation (Andronov–Hopf), and it shows different localized structures. For pulse-type structures, we review approximation scheme that enables us to compute some properties like their existence range. From scheme, obtain conditions pulses upper limit a control parameter. When study width limit, analytical expression related transition between fronts. This fact...
We present an experimental study on the statistical properties of injected power needed to maintain inelastic ball bouncing constantly a randomly accelerating piston in presence gravity. compute at each collision with moving by measuring velocity and force exerted ball. The probability density function has its most probable value close zero displays two asymmetric exponential tails, depending restitution coefficient, acceleration, frequency content. This distribution can be deduced from...
A new pulsed RF plasma reactor has been used for the synthesis of graphene-based nanomaterials under atmopheric pressure conditions. This is an environmental-friendly system, not using catalyst and where no hazardous components are generated. Nanographenes formed upon treatment some hydrocarbons (cyclohexane, cyclohexene, heptane, hexane, pentane, toluene), while N-containing organic compounds (cyclohexylamine, pyridine, dipropylamine, triethylamine) resulted in N-doped graphenes. Synthesis...