A5042344146- Immune Cell Function and Interaction
- Virus-based gene therapy research
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Polyomavirus and related diseases
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Research Studies
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Immune responses and vaccinations
- Acute Myeloid Leukemia Research
- Reproductive System and Pregnancy
- Neuroblastoma Research and Treatments
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Heart Failure Treatment and Management
- MicroRNA in disease regulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
University of Virginia
2023
Kansai Medical University
2023
Yonsei University
2013-2019
RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe establishment of gene-knockout mice zebrafish by injection RGENs as Cas9 protein:guide RNA complexes or mRNA plus guide into one-cell-stage embryos both species. efficiently generated germline transmittable mutations up to 93% newborn with minimal toxicity. RGEN-induced mouse Prkdc gene that encodes an enzyme critical for DNA...
Abstract Regulatory T (Treg) cells act as terminators of cell immuniy during acute phase viral infection; however, their role and suppressive mechanism in chronic infection are not completely understood. In this study, we compared the phenotype function Treg or with lymphocytic choriomeningitis virus. Chronic infection, unlike led to a large expansion upregulation programmed death-1 (PD-1). from chronically infected mice (chronic cells) displayed greater capacity for inhibiting both CD8+...
Clonal hematopoiesis (CH), which results from an array of nonmalignant driver gene mutations, can lead to altered immune cell function and chronic disease, has been associated with worse outcomes in patients heart failure (HF) reduced ejection fraction. However, the role CH prognosis HF preserved fraction (HFpEF) understudied. This study aimed characterize HFpEF elucidate its causal a murine model.
The phosphoinositide 3-kinase-Akt signaling pathway is essential to many biological processes, including cell proliferation, survival, metabolism, and angiogenesis, under pathophysiological conditions. Although 3-phosphoinositide-dependent kinase 1 (PDK1) a primary activator of Akt at the plasma membrane, optimal activation mechanism remains unclear. We report that adhesion molecule with IgG-like domain 2 (AMIGO2) novel scaffold protein regulates PDK1 membrane localization activation. Loss...
MicroRNA (miR)-150 is a developmental regulator of several immune-cell types, but its role in CD8+ T cells largely unexplored. Here, we show that miR-150 regulates the generation memory cells. After acute virus infection, knockout (KO) mice exhibited an accelerated differentiation into and improved production effector cytokines. Additionally, KO displayed enhanced recall response protection against infections with another bacteria. We found forkhead box O1 (Foxo1) cell-specific transcription...
Monocyte-derived dendritic cells (moDCs) have been shown to robustly expand during infection; however, their roles in anti-infectious immunity remain unclear. Here, we found that moDCs were dramatically increased the secondary lymphoid organs acute LCMV infection an interferon-γ (IFN-γ)-dependent manner. We also priming by enhanced differentiation of memory CD8+ T compared primed conventional (cDCs) through upregulation Eomesodermin (Eomes) and cell factor-1 (TCF-1) expression cells....
The importance of natural killer (NK) cells in the early immune response to viral or bacterial infection is well known. However, phenotype, function, and physiologic role NK during late stage persistent have not been extensively studied. Here, we characterized mice persistently infected with lymphocytic choriomeningitis virus clone 13 showed that contrast from acutely uninfected mice, chronically expressed a terminally differentiated stronger cytotoxicity, reduced inhibitory receptor...
Regulation of intracellular Ca2+ signaling is a major determinant CD8+ T cell responsiveness, but the mechanisms underlying this regulation levels, especially in naïve cells, are not fully defined. Here, we showed that microRNA-150 (miR-150) controls levels cells required for activation by suppressing TMEM20, negative regulator extrusion. miR-150 deficiency increased TMEM20 expression, which resulted cells. The subsequent increase induced expression anergy-inducing genes, such as Cbl-b,...
Abstract Small cell lung cancer (SCLC) is aggressive with limited therapeutic options. Despite recent advances in targeted therapies and immunotherapies, therapy resistance a recurring issue, which might be partly due to tumor plasticity, change fate. Nonetheless, the mechanisms underlying plasticity immune evasion SCLC remain elusive. CRACD, capping protein inhibitor that promotes actin polymerization, frequently inactivated SCLC. Cracd knockout (KO) transforms preneoplastic cells into...
Abstract Regulatory T (Treg) cells act as terminators of cell immunity during acute phase viral infection; however, their role and suppressive mechanism in chronic infection are not completely understood. In this study, we compared the phenotype function Treg or with lymphocytic choriomeningitis virus. Chronic infection, unlike led to a large expansion upregulation programmed death-1 (PD-1). from chronically infected mice (chronic cells) displayed greater capacity for inhibiting both CD8+...
Quantitative PCR and plaque assay are powerful virological techniques used to measure the load of defective or infectious virus in mouse human.However, these methods display limitations such as cross contamination long run-time.Here, we describe a novel technique termed semi-functional quantitative flow cytometry (SFQF) for accurate estimation quantity lymphocytic choriomeningitis (LCMV).LCMV titration method using was previously developed but has technical shortcomings, owing less optimized...
Abstract Background: Mutations in the epidermal growth factor receptor (EGFR) gene are associated with increased sensitivity of lung adenocarcinoma to tyrosine kinase inhibitor (TKI) such as gefitinib. During gefitinib treatment, however, tumor becomes resistant by acquiring specific types resistance via somatic changes. Although underlying mechanisms for acquired include amplification c-Met and secondary mutation EGFR genes, there still unknown that cannot be explained two genetic Here, we...
<div>Abstract<p>The importance of natural killer (NK) cells in the early immune response to viral or bacterial infection is well known. However, phenotype, function, and physiologic role NK during late stage persistent have not been extensively studied. Here, we characterized mice persistently infected with lymphocytic choriomeningitis virus clone 13 showed that contrast from acutely uninfected mice, chronically expressed a terminally differentiated stronger cytotoxicity, reduced...
<p>Figure S1. shows NK cell phenotypes at different time points after chronic LCMV infection.</p>
<div>Abstract<p>The importance of natural killer (NK) cells in the early immune response to viral or bacterial infection is well known. However, phenotype, function, and physiologic role NK during late stage persistent have not been extensively studied. Here, we characterized mice persistently infected with lymphocytic choriomeningitis virus clone 13 showed that contrast from acutely uninfected mice, chronically expressed a terminally differentiated stronger cytotoxicity, reduced...
<p>Figure S1. shows NK cell phenotypes at different time points after chronic LCMV infection.</p>
<p>Figure S3. shows the analysis of tumor infiltrating lymphocytes in subcutaneous EG7 model.</p>
<p>Figure S2. shows the IFN-γ production of NK cells stimulated by cytokines.</p>
<p>Figure S2. shows the IFN-γ production of NK cells stimulated by cytokines.</p>
<p>Figure S3. shows the analysis of tumor infiltrating lymphocytes in subcutaneous EG7 model.</p>
<p>Figure S4. shows the subcutaneous tumor growth in mice at different time points after chronic LCMV infection.</p>
<p>Figure S5. shows the efficacy of depleting antibodies and viral titers after treatment antibodies.</p>
<p>Figure S6. shows that there is no direct antitumor effect by LCMV replication.</p>