A5061756371- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- RNA Research and Splicing
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Toxin Mechanisms and Immunotoxins
- Advanced Electron Microscopy Techniques and Applications
- Nuclear Physics and Applications
- Molecular Biology Techniques and Applications
- Genetics, Bioinformatics, and Biomedical Research
- Peptidase Inhibition and Analysis
- Mass Spectrometry Techniques and Applications
- NMR spectroscopy and applications
- Crystallography and molecular interactions
- Advanced NMR Techniques and Applications
- Aluminum toxicity and tolerance in plants and animals
- Glycosylation and Glycoproteins Research
- Electron Spin Resonance Studies
- Microbial Natural Products and Biosynthesis
- Enterobacteriaceae and Cronobacter Research
- Psychotherapy Techniques and Applications
Yale University
2015-2024
Whitney Museum of American Art
1997-2020
Howard Hughes Medical Institute
2001-2020
Google (United States)
2017
Queen Elizabeth Hospital
2017
Pain Management Institute
2016
University of New Haven
1983-2013
Seattle University
2008
Melinta Therapeutics (United States)
2008
University of Liverpool
2007
The large ribosomal subunit catalyzes peptide bond formation and binds initiation, termination, elongation factors. We have determined the crystal structure of from Haloarcula marismortui at 2.4 angstrom resolution, it includes 2833 subunit's 3045 nucleotides 27 its 31 proteins. domains RNAs all irregular shapes fit together in ribosome like pieces a three-dimensional jigsaw puzzle to form large, monolithic structure. Proteins are abundant everywhere on surface except active site where...
Using the atomic structures of large ribosomal subunit from Haloarcula marismortui and its complexes with two substrate analogs, we establish that ribosome is a ribozyme address catalytic properties all-RNA active site. Both analogs are contacted exclusively by conserved RNA (rRNA) residues domain V 23 S rRNA; there no protein side-chain atoms closer than about 18 angstroms to peptide bond being synthesized. The mechanism synthesis appears resemble reverse acylation step in serine proteases,...
Analysis of the 2.4-A resolution crystal structure large ribosomal subunit from Haloarcula marismortui reveals existence an abundant and ubiquitous structural motif that stabilizes RNA tertiary quaternary structures. This is termed A-minor motif, because it involves insertion smooth, minor groove edges adenines into neighboring helices, preferentially at C-G base pairs, where they form hydrogen bonds with one or both 2' OHs those pairs. motifs stabilize contacts between interactions loops...
The crystal structure of the monoclinic form yeast phenylalanine tRNA has been redetermined at a resolution 1.93 Å. tRNAphe described here is more accurate than its predecessors not only because it incorporates higher data, but also refined using techniques that had developed when were determined 20 years ago. Å version this differs interestingly from in details. In loop regions particularly, backbone torsion angles new are same as those reported earlier. Several divalent cation binding...
An analysis of the small-angle scattering problem based on Shannon sampling is presented. It shown that this approach leads to an accurate assessment information contained in a given data set and rigorous estimates errors inherent parameters derived therefrom.
The oxazolidinone antibacterials target the 50S subunit of prokaryotic ribosomes. To gain insight into their mechanism action, crystal structure canonical oxazolidinone, linezolid, has been determined bound to Haloarcula marismortui subunit. Linezolid binds A-site, near catalytic center, which suggests that inhibition involves competition with incoming A-site substrates. These results provide a structural basis for discovery improved oxazolidinones active against emerging drug-resistant...
Both monovalent cations and magnesium ions are well known to be essential for the folding stability of large RNA molecules that form complex compact structures. In atomic structure ribosomal subunit from Haloarcula marismortui , we have identified 116 88 bound principally rRNA. Although rRNA structures which these metal bind highly idiosyncratic, a few common principles emerged identities specific functional groups coordinate them. The nonbridging oxygen phosphate group is most inner shell...
The relative positions of the centers mass 21 proteins 30S ribosomal subunit from Escherichia coli have been determined by triangulation using neutron scattering data. resulting map quaternary structure small is presented, and comparisons are made with structural data other sources.
The large ribosomal subunit catalyzes peptide bond formation and will do so by using small aminoacyl- peptidyl-RNA fragments of tRNA. We have refined at 3-Å resolution the structures both A P site substrate product analogues, as well an intermediate analogue, bound to Haloarcula marismortui 50S subunit. substrate, CCA-Phe-caproic acid–biotin, binds equally sites, but in presence sparsomycin only site. CCA portions these analogues are identically either or loop 23S rRNA. Combining separate...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolution structure of an unusually stable RNA tetraplex containing G- and U-quartet structuresChaejoon Cheong Peter B. MooreCite this: Biochemistry 1992, 31, 36, 8406–8414Publication Date (Print):September 15, 1992Publication History Published online1 May 2002Published inissue 15 September 1992https://pubs.acs.org/doi/10.1021/bi00151a003https://doi.org/10.1021/bi00151a003research-articleACS PublicationsRequest reuse permissionsArticle...
The 50S subunit of the ribosome catalyzes peptidyl-transferase reaction protein synthesis. We have generated X-ray crystallographic electron density maps large ribosomal from Haloarcula marismortui at various resolutions up to 9 Å using data crystals that diffract 3 Å. Positioning a 20 resolution EM image these particles in crystal lattice produced phases accurate enough locate bound heavy atoms three derivatives difference Fourier maps, thus demonstrating correctness model and its placement...