A5025031013- Mitochondrial Function and Pathology
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Neuroscience and Neuropharmacology Research
- CRISPR and Genetic Engineering
- Aquaculture disease management and microbiota
- Neuroinflammation and Neurodegeneration Mechanisms
- S100 Proteins and Annexins
- Saffron Plant Research Studies
- Air Quality Monitoring and Forecasting
- Neurological Disease Mechanisms and Treatments
- Receptor Mechanisms and Signaling
- Genetic Neurodegenerative Diseases
- Air Quality and Health Impacts
- Photosynthetic Processes and Mechanisms
- Lipid Membrane Structure and Behavior
- Immune Response and Inflammation
- Cardiomyopathy and Myosin Studies
- Genetics and Neurodevelopmental Disorders
- Invertebrate Immune Response Mechanisms
Ajou University
2013-2024
Max Planck Institute for Biology of Ageing
2013-2015
Dong-A University
2013
Karolinska Institutet
2005-2011
Max Planck Society
2010
Korea Advanced Institute of Science and Technology
1996-2008
Sungkyunkwan University
2005
Samsung (South Korea)
2005
Kyoto University
2000
University of Toronto
2000
Buforin II is a 21-aa potent antimicrobial peptide that forms, in hydrophobic medium, an amphipathic structure consisting of N-terminal random coil region (residues 1–4), extended helical 5–10), hinge (residue 11), and C-terminal regular α-helical 12–21). To elucidate the structural features buforin are required for its activity, we synthesized series N- C-terminally truncated or amino acid-substituted synthetic analogs examined their activity mechanism action. Deletion increased...
Significance Altered expression of mitochondrial DNA (mtDNA) is heavily implicated in human disease and aging, but the basic organizational unit mtDNA, nucleoid, poorly understood. Here, we have used a combination biochemistry, superresolution microscopy, electron microscopy to show that mammalian nucleoids an irregular ellipsoidal shape typically contain single copy mtDNA. Furthermore, nucleoid ultrastructure independent cellular mtDNA number core structure formed by cross-strand binding...
In response to epidermal injury, Parasilurus asotus , a catfish, secreted strong antimicrobial peptide into the epithelial mucosal layer. The molecular mass of peptide, named parasin I, was 2000.4 Da, as determined by matrix‐associated laser desorption ionization spectrometry. complete amino acid sequence which automated Edman degradation, Lys‐Gly‐Arg‐Gly‐Lys‐Gln‐Gly‐Gly‐Lys‐Val‐Arg‐Ala‐Lys‐Ala‐Lys‐Thr‐Arg‐Ser‐Ser. Eighteen 19 residues in I were identical N‐terminal buforin 39‐residue...
Buforin 2 is an antimicrobial peptide discovered in the stomach tissue of Asian toad Bufo bufo gargarizans. The 21-residue with +6 net charge shows activity order magnitude higher than that magainin 2, a membrane-permeabilizing from Xenopus laevis [Park, C. B., Kim, M. S., and S. (1996) Biochem. Biophys. Res. Commun. 218, 408−413]. In this study, we investigated interactions buforin phospholipid bilayers comparison to obtain insight into mechanism action 2. Equipotent Trp-substituted...
Mitochondrial transcription factor A (TFAM) is compacting mitochondrial DNA (dmtDNA) into nucleoids and directly controls mtDNA copy number. Here, we show that the TFAM-to-mtDNA ratio critical for maintaining normal expression in different mouse tissues. Moderately increased TFAM protein levels increase number but a maintained resulting unaltered whole animal metabolism. Mice ubiquitously expressing very high develop pathology leading to deficient oxidative phosphorylation (OXPHOS) early...
A novel antimicrobial peptide, named misgurin, was isolated and characterized from the loach (mudfish), Misgurnus anguillicaudatus . The 21‐amino‐acid peptide with a molecular mass of 2502 Da purified to homogeneity using heparin‐affinity column C18 reverse‐phase gel‐permeation high‐performance liquid chromatography. complete amino acid sequence which determined by an automated sequencer, Arg–Gln–Arg–Val–Glu–Glu–Leu–Ser–Lys–Phe–Ser–Lys–Lys–Gly–Ala–Ala–Ala–Arg–Arg–Arg–Lys. Misgurin is...
Regulation of mitochondrial DNA (mtDNA) expression is critical for the control oxidative phosphorylation in response to physiological demand, and this regulation often impaired disease aging. We have previously shown that transcription termination factor 3 (MTERF3) a key regulator represses mtDNA mouse, but its molecular mode action has remained elusive. Based on hypothesis regulatory mechanisms are conserved metazoans, we analyzed Mterf3 knockout knockdown flies. demonstrate here decreased...
We investigated the cytotoxic activities of crocin and crocetin, 2 major carotenoids isolated from stigma Crocus sativus (saffron), on 5 human cancer cell lines proposed their possible anticancer mechanisms. Crocetin, a glycosylated carotenoid, showed approximately 5- to 18-fold higher cytotoxicity than crocin, carboxylic carotenoid (IC50 0.16-0.61 mmol/L for crocetin vs. 2.0-5.5 crocin). This suggests that structural differences account different efficacies between them....
Abstract Here we report the expansion of genetic code Mus musculus with various unnatural amino acids including N ɛ -acetyl-lysine. Stable integration transgenes encoding an engineered -acetyl-lysyl-tRNA synthetase (AcKRS)/tRNA Pyl pair into mouse genome enables site-specific incorporation a target protein in response to amber codon. We demonstrate temporal and spatial control acetylation organs transgenic using recombinant green fluorescent (GFPuv) as model protein. This strategy will...
Abstract The intestinal epithelium forms a first line of innate host defense by secretion proteins with antimicrobial activity against microbial infection. Despite the extensive studies on in many gastrointestinal tracts, little is known about system stomach. potent peptide buforin I, consisting 39 aa, was isolated recently from stomach tissue an Asian toad, Bufo bufo gargarizans. In this study we examined mechanism I production toad tissue. Buforin produced action pepsin isozymes, named Ca...
Genetic mutations that affect mitochondrial function often cause skeletal muscle dysfunction. Here, we used mice with skeletal-muscle-specific disruption of the nuclear gene for transcription factor A ( Tfam ) to study whether changes in cellular Ca 2+ handling is part mechanism dysfunction myopathy. Force measurements were combined cytosolic , and membrane potential reactive oxygen species intact, adult fibres. The results show reduced sarcoplasmic reticulum (SR) storage capacity KO muscles...