A5041420957- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Virus-based gene therapy research
- Plant Molecular Biology Research
- Pancreatic and Hepatic Oncology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Adenosine and Purinergic Signaling
- Corneal Surgery and Treatments
- Cancer Genomics and Diagnostics
- Corneal surgery and disorders
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Poxvirus research and outbreaks
- Pharmacological Effects of Natural Compounds
- Plant Reproductive Biology
- Natural product bioactivities and synthesis
- Melanoma and MAPK Pathways
- Calcium signaling and nucleotide metabolism
- Glaucoma and retinal disorders
- Endoplasmic Reticulum Stress and Disease
- Agronomic Practices and Intercropping Systems
- Ginger and Zingiberaceae research
- Plant Pathogenic Bacteria Studies
- Renal cell carcinoma treatment
University of Missouri
2017-2025
Moffitt Cancer Center
2020-2025
Gangneung–Wonju National University
2025
Gyeongsang National University
2008-2024
Can Tho University
2023
University of Minnesota
2023
Center for Life Sciences
2023
Dankook University
2016-2023
Icahn School of Medicine at Mount Sinai
2023
Ho Chi Minh City University of Science
2023
In addition to acting as a cellular energy source, ATP can also act damage-associated molecular pattern in both animals and plants. Stomata are leaf pores that control gas exchange and, therefore, impact critical functions such photosynthesis, drought tolerance, the preferred entry point for pathogens. Here we show of leads rapid closure stomata enhanced resistance bacterial pathogen Psuedomonas syringae. This response is mediated by recognition receptor DORN1, followed direct...
The potential benefits or the tissue-damaging effects of inflammatory response after central nervous system injuries have long been disputed. Recent studies noted that substance P (SP), a neuropeptide, plays an important role in wound-healing process by recruiting bone marrow stem cells to injured tissue. In this study, we examined whether SP can enhance recovery from spinal cord injury (SCI) Sprague-Dawley rats through its known function cell mobilization and/or modulation inflammation. We...
Although heat-shock transcription factors are well characterized in the heat stress-related pathway, they poorly understood other stress responses. Here, we functionally AtHsfA6a presence of exogenous abscisic acid (ABA) and under high salinity dehydration conditions. expression normal conditions is very low, but was highly induced by ABA, NaCl drought. Unexpectedly, levels transcript were not significantly altered cold stresses. Electrophoretic mobility shift assays transient...
Abstract The mevalonate pathway plays a critical role in multiple cellular processes both animals and plants. In plants, the products of this impact growth development, as well response to environmental stress. A forward genetic screen Arabidopsis thaliana using Ca 2+ -imaging identified kinase (MVK) component plant purinergic signaling. MVK interacts directly with extracellular ATP (eATP) receptor P2K1 is phosphorylated by eATP. Mutation P2K1-mediated phosphorylation sites eliminates...
purpose. To determine whether keratoconus (KC) corneal fibroblast cultures have increased reactive oxygen species (ROS) production and are more susceptible to stress-related challenges. methods. Normal (n = 9) KC 10) stromal were incubated in either neutral- or low-pH conditions, with without hydrogen peroxide. Catalase activities measured a fluorescent substrate assay. Superoxide ROS/reactive nitrogen (RNS) productions determined an amine-reactive green-dye assay...
To evaluate the short-term in vitro safety of bevacizumab (Avastin) human retinal pigment epithelial (ARPE-19), rat neurosensory (R28), and microvascular endothelial (HMVECad) cells.ARPE-19 R28 cells were treated with 0.125 mg/mL, 0.25 0.50 1 mg/mL for 2, 6, 24 hours. HMVECad 5 ng/mL vascular growth factor (VEGF) either hours or a nonspecific purified immunoglobulin (IgG) Cell viability was measured using trypan blue dye exclusion assay.The cell viabilities ARPE-19 cells, not significantly...
It is unclear whether the oxidative damage found in keratoconus (KC) corneas results from innate defects of corneal fibroblasts or due to excessive environmental challenges encountered by patient with KC. The purpose this study was explore KC cells have inherent, exaggerated hypersensitivity stressors.Normal and stromal were incubated neutral low-pH conditions, without hydrogen peroxide (H(2)O(2)). Reactive oxygen/nitrogen species (ROS/RNS) production measured...
Abstract Mitogen-activated protein (MAP) kinase signaling cascades play important roles in eukaryotic defense against various pathogens. Activation of the extracellular ATP (eATP) receptor P2K1 triggers MAP 3 and 6 (MPK3/6) phosphorylation, which leads to an elevated plant response. However, mechanism by activates MAPK cascade is unclear. In this study, we show that Arabidopsis thaliana, phosphorylates Raf-like (MAPKKK) INTEGRIN-LINKED KINASE 5 (ILK5) on serine 192 presence eATP. The...
Reactive oxygen species contribute to the development of various human diseases. Ischemia is characterized by both significant oxidative stress and characteristic changes in antioxidant defense mechanism. Heat shock protein 27 (HSP27) has a potent ability increase cell survival response stress. In present study, we have investigated protective effects PEP-1-HSP27 against death ischemic insults. When fusion was added culture medium astrocyte primary neuronal cells, it rapidly entered cells...
In Chl biosynthesis, aerobic Mg-protoporphyrin IX monomethyl ester (MPE) cyclase is a key enzyme involved in the synthesis of protochlorophyllide a, and its membrane-bound component known to be encoded by homologs CHL27 photosynthetic bacteria, green algae plants. Here, we report that Arabidopsis chl27-t knock-down mutant exhibits retarded growth chloroplast developmental defects are caused damage PSII reaction centers. The contains T-DNA insertion within promoter dramatically reduces mRNA...
Herbaspirillum seropedicae is an endophytic bacterium that establishes association with a variety of plants, such as rice, corn, and sugarcane, can significantly increase plant growth. H. produces polyhydroxybutyrate (PHB), stored in the form insoluble granules. Little information available on possible role PHB bacterial root colonization or growth promotion. To investigate whether important for we inoculated roots Setaria viridis strain SmR1 mutants defective production (ΔphaP1, ΔphaP1...
724 Background: RMC-9805 is a potent, oral, RAS(ON) G12D-selective, covalent, tri-complex inhibitor targeting the active, GTP-bound state of oncogenic RAS G12D isoforms. Despite low response rates to current standard care (SOC), no RAS-targeted therapy approved for PDAC. Methods: In this Phase 1 study (NCT06040541), patients with previously treated, advanced KRAS solid tumors received escalating doses (150-1200 mg once daily [QD] or 300-600 twice [BID]). Treatment cycles were every 3 wks....
In eukaryotic organisms, protein kinases regulate diverse activities and signaling pathways through phosphorylation of specific substrates. Isolating characterizing kinase substrates is vital for defining downstream pathways. The Kinase Client (KiC) assay an in vitro synthetic peptide LC-MS/MS that has enabled identification (i.e., clients) various kinases. For example, previous use a 2,100-member (2k) library identified the extracellular ATP receptor-like kinase, P2K1. Many P2K1 clients...
Pancreatic ductal adenocarcinoma is projected to become the second leading cause of cancer-related deaths by 2040, with highest disease burden expected amongst Non-Hispanic Blacks. One most significant predictors poor outcomes presence cancer-associated cachexia (CCa). Yet, race- and ethnicity-specific biomarkers for early CCa diagnosis are lacking. Thus, evaluated a panel candidate in diverse cohort pre-treatment serum. Our study shows that GDF-15 was associated severity, superior standard...
In plants, root hairs undergo a highly polarized form of cell expansion called tip-growth, in which wall deposition is restricted to the hair apex. order identify essential cellular components that might have been missed earlier genetic screens, we identified conditional temperature-sensitive (ts) mutants by ethyl methanesulfonate mutagenesis Arabidopsis thaliana. Here, describe one these mutants, feronia-temperature sensitive (fer-ts). Mutant fer-ts seedlings were unaffected at normal...
The ubiquitin/26S proteasome system is a crucial regulatory mechanism that governs various cellular processes in plants, including signal transduction, transcriptional regulation, and responses to biotic abiotic stressors. Our study shows the RING-H2-type E3 ubiquitin ligase, Arabidopsis Tóxicos en Levadura 2 (ATL2), involved response fungal pathogen infection. Under normal growth conditions, expression of ATL2 gene low, but it rapidly significantly induced by exogenous chitin. Additionally,...
Summary Phosphoinositides ( PI s) are essential metabolites which generated by various lipid kinases and rapidly respond to a variety of environmental stimuli in eukaryotes. One the precursors important regulatory s, phosphatidylinositol (PtdIn) 4‐phosphate, is synthesized PtdIns 4‐kinases 4K). Despite its wide distribution eukaryotes, role plants remains largely unknown. Here, we show that activity At 4K γ 3 gene, an Arabidopsis thaliana ) type II PtdIn 4‐kinase, regulated DNA demethylation...