A5089526684- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Cellular transport and secretion
- Plant biochemistry and biosynthesis
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Plant Parasitism and Resistance
- ATP Synthase and ATPases Research
- Biochemical and Structural Characterization
- Toxin Mechanisms and Immunotoxins
- Light effects on plants
- Plant tissue culture and regeneration
- Microbial Natural Products and Biosynthesis
- Plant Virus Research Studies
- Biochemical and biochemical processes
- 14-3-3 protein interactions
- Horticultural and Viticultural Research
- Phytochemicals and Antioxidant Activities
- Bioactive natural compounds
University of Oxford
2015-2024
VIB-UGent Center for Plant Systems Biology
2011-2017
National Centre for Biological Sciences
2015
Ghent University
2009-2014
The Ohio State University
2003-2013
Vlaams Instituut voor Biotechnologie
2009-2012
Plants produce a very large number of specialized compounds that must be transported from their site synthesis to the sites storage or disposal. Anthocyanin accumulation has provided powerful system elucidate molecular and cellular mechanisms associated with intracellular trafficking phytochemicals. Benefiting unique fluorescent properties anthocyanins, we show here in Arabidopsis (Arabidopsis thaliana), one route for anthocyanin transport vacuole involves vesicle-like structures shared...
Abstract Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance this process, machinery that CME in plants is largely unknown. In mammals, heterotetrameric ADAPTOR PROTEIN COMPLEX-2 (AP-2) required for formation clathrin-coated vesicles at plasma membrane (PM). Although existence AP-2 has been predicted Arabidopsis thaliana, biochemistry functionality complex still...
Anthocyanins are flavonoid pigments that accumulate in the large central vacuole of most plants. Inside vacuole, anthocyanins can be found uniformly distributed or as part sub-vacuolar pigment bodies, Anthocyanic Vacuolar Inclusions (AVIs). Using Arabidopsis seedlings grown under anthocyanin-inductive conditions a model to understand how AVIs formed, we show here accumulation strongly correlates with formation cyanidin 3-glucoside (C3G) and derivatives. mutants fail glycosylate...
The R2R3 MYB transcription factor C1 requires the basic helix-loop-helix R as an essential co-activator for of maize anthocyanin genes. In contrast, protein P1 activates a subset C1-regulated genes independently R. Substitution six amino acids in with results P1*, whose activity on and P1-regulated is R-dependent or R-enhanced, respectively. We have used P1* combination various promoters to uncover two mechanisms function. On synthetic that contain only C1/P1 binding sites, C1. This function...
Summary In addition to the classical functions of flavonoids in response biotic/abiotic stress conditions, these phenolic compounds have been implicated modulation various developmental processes. These findings suggest that are more integral components plant signaling machinery than traditionally recognized. To understand how flux through flavonoid pathway affects cellular processes, we used wild‐type and chalcone isomerase mutant ( transparent testa 5 , tt5 ) seedlings grown under...
Endocytosis is a ubiquitous cellular process that characterized well in animal cells culture but poorly across intact, functioning tissue. Here, we analyze endocytosis throughout the Arabidopsis thaliana root using three classes of probes: lipophilic dye, tagged transmembrane proteins, and lipid-anchored protein. We observe stratified distribution endocytic processes. A clathrin-dependent pathway internalizes proteins functions all cell layers, while sterol-dependent, clathrin-independent...
Plant organogenesis requires control over division planes and anisotropic cell wall growth, which each require spatial patterning of cells. Polyhedral plant cells can display complex in individual faces are established as biochemically distinct domains by endomembrane trafficking. We now show that, during organogenesis, the Arabidopsis system specifies an important additional cellular domain: geometric edges. Previously unidentified membrane vesicles lying immediately beneath plasma at edges...
Protein phosphatase 2A acts as both a positive and negative regulator of brassinosteroid signaling, depending on where in the pathway acts.
Plant pigmentation is affected by a variety of factors. Light, an important plant developmental signal, influences the accumulation anthocyanins primarily through activation transcription factors that regulate flavonoid biosynthetic pathway. In this study, we utilized maize Black Mexican Sweet (BMS) cells expressing R and C1 regulators anthocyanin biosynthesis from light-insensitive promoter as means to investigate existence additional levels control light.BMS CaMV 35S constitutive...
Morphogenesis in plants depends critically on directional (anisotropic) growth. This occurs principally perpendicular to the net orientation of cellulose microfibrils (CMFs), which is turn controlled by cortical microtubules (CMTs). In young lateral roots Arabidopsis thaliana, growth anisotropy also RAB-A5c, a plant-specific small GTPase that specifies membrane trafficking pathway geometric edges cells. Here we investigate functional relationship between structural at faces and RAB-A5c...
Catalytically impaired chalcone isomerases having no appreciable activity in vitro retain the ability to robustly promote flavonoid biosynthesis planta.
Little is known regarding the trafficking mechanisms of small molecules within plant cells. It remains to be established whether phytochemicals are transported by pathways similar those used proteins, or expansion metabolic in plants was associated with evolution novel pathways. In this paper, we exploited induction green and yellow auto-fluorescent compounds maize cultured cells P1 transcription factor investigate their targeting cell wall vacuole, respectively. We investigated accumulation...
Abstract Cytokinesis is a key process in the development of multicellular organisms, through both formative and proliferative divisions. In land plants, successful cytokinesis requires precise targeting Golgi-derived vesicles to future division plane by phragmoplast, cytoskeletal structure that undergoes continuous remodelling. Endomembrane trafficking phragmoplast remodelling are tightly coupled, indicating existence active crosstalk. However, although many molecular regulators membrane...
A subset of genes in Arabidopsis thaliana is known to be up-regulated response a wide range different environmental stress factors. However, not all these are characterized as yet with respect their functions. In this study, we used transgenic knockout, overexpression and reporter gene approaches try elucidate the biological roles five unknown multiple-stress responsive Arabidopsis. The selected have following locus identifiers: At1g18740, At1g74450, At4g27652, At4g29780 At5g12010. Firstly,...
ABSTRACT The occasion of Company Biologists' workshop ‘Cellular gateways: expanding the role endocytosis in plant development’ on 22–25 April 2018, at Wiston House, an Elizabethan mansion West Sussex, England, witnessed stimulating and lively discussions mechanism functions cells. was organized by Jenny Russinova, Daniël Van Damme (both VIB/University Ghent, Belgium) Takashi Ueda (National Institute for Basic Biology, Okazaki, Japan), aimed to bridge gap knowledge about endocytic machinery...
Abstract Background Natural products have numerous medicinal applications and play important roles in the biology of organisms that accumulate them. Few methods are currently available for identifying proteins bind to small molecules, therefore discovery cellular targets natural with pharmacological activity continues pose a significant challenge drug validation. Similarly, identification enzymes participate biosynthesis or modification remains formidable bottleneck metabolic engineering....