A5005470171- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Cellular transport and secretion
- Legume Nitrogen Fixing Symbiosis
- Toxin Mechanisms and Immunotoxins
- Research in Cotton Cultivation
- Fungal and yeast genetics research
- Monoclonal and Polyclonal Antibodies Research
- Plant-Microbe Interactions and Immunity
- Transgenic Plants and Applications
- Ubiquitin and proteasome pathways
- Agronomic Practices and Intercropping Systems
- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Bioenergy crop production and management
Charles University
2012-2024
Czech Academy of Sciences, Institute of Experimental Botany
2009-2024
Czech Academy of Sciences
2008-2012
Abstract The exocyst, an octameric tethering complex and effector of Rho Rab GTPases, facilitates polarized secretion in yeast animals. Recent evidence implicates three plant homologs exocyst subunits (SEC3, SEC8, EXO70A1) cell morphogenesis. Here, we provide genetic, biological, biochemical that these other predicted function together vivo Arabidopsis thaliana. Double mutants (sec5 exo70A1 sec8 exo70A1) show a synergistic defect etiolated hypocotyl elongation. Mutants SEC5, SEC6, SEC15a...
Recently, the octameric vesicle-tethering complex exocyst was found in plants and its importance for Arabidopsis morphogenesis demonstrated. Exo70 subunits plants, unlike yeasts mammals, are represented by a multigene family, comprising 23 members Arabidopsis. For Exo70B2 Exo70H1 paralogues, transcriptional up-regulation confirmed on treatment with an elicitor peptide, elf18, derived from bacterial elongation factor. Their ability to participate formation inferred interaction of both Exo70s...
Cell reproduction is a complex process involving whole cell structures and machineries in space time, resulting regulated distribution of endomembranes, organelles, genomes between daughter cells. Secretory pathways supported by the activity Golgi apparatus play crucial role cytokinesis plants. From onset phragmoplast initiation to maturation plate, delivery secretory vesicles necessary sustain successful separation. Tethering at plasma membrane mediated evolutionarily conserved octameric...
Exocyst is an evolutionarily conserved vesicle tethering complex functioning especially in the last stage of exocytosis. Homologs its eight canonical subunits - Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84 were found also higher plants confirmed to form complexes vivo, participate cell growth including polarized expansion pollen tubes root hairs. Here we present results a phylogenetic study land plant exocyst encoded by selection completely sequenced genomes representing variety...
In land plants polar auxin transport is one of the substantial processes guiding whole plant polarity and morphogenesis. Directional fluxes are mediated by PIN efflux carriers, polarly localized at plasma membrane. The polarization exocytosis in yeast animals assisted exocyst: an octameric vesicle-tethering complex effector Rab Rho GTPases. Here we show that rootward compromised roots Arabidopsis thaliana loss-of-function mutants EXO70A1 exocyst subunit. recycling PIN1 PIN2 proteins from...
The exocyst, a eukaryotic tethering complex, coregulates targeted exocytosis as an effector of small GTPases in polarized cell growth. In land plants, several exocyst subunits are encoded by double or triple paralogs, culminating tens EXO70 paralogs. Out 23 Arabidopsis thaliana isoforms, we analyzed seven isoforms expressed pollen. Genetic and microscopic analyses single mutants EXO70A2, EXO70C1, EXO70C2, EXO70F1, EXO70H3, EXO70H5, EXO70H6 genes revealed that only loss-of-function EXO70C2...
The exocyst, an evolutionarily conserved secretory vesicle-tethering complex, spatially controls exocytosis and membrane turnover in fungi, metazoans plants. exocyst subunit EXO70 exists multiple paralogs land plants, forming three clades with assumed distinct roles. Here we report functional analysis of the first moss to be studied, Physcomitrella patens PpEXO70.3d (Pp1s97_91V6), from the, as yet, poorly characterized EXO70.3 clade. Following phylogenetic confirm presence ancestral plant...
RAB GTPases are important directional regulators of intracellular vesicle transport. Membrane localization is mediated by C-terminal double geranylgeranylation. This post-translational modification catalyzed the α-β-heterodimer catalytic core geranylgeranyl transferase (RAB-GGT), which cooperates with escort protein (REP) that presents a nascent RAB. Here, we show RAB-geranylgeranylation activity significantly reduced in two homozygous mutants major Arabidopsis β-subunit RAB-GGT (AtRGTB1),...
The collet (root-hypocotyl junction) region is an important plant transition zone between soil and atmospheric environments. Despite its crucial importance for development, little known about how this specified. Here we document the involvement of exocyst complex in process. exocyst, octameric tethering complex, participates secretion membrane recycling central to numerous cellular developmental processes, such as growth root hairs, cell expansion, PIN auxin efflux carriers many others. We...
Abstract Cytokinesis is a key process in the development of multicellular organisms, through both formative and proliferative divisions. In land plants, successful cytokinesis requires precise targeting Golgi-derived vesicles to future division plane by phragmoplast, cytoskeletal structure that undergoes continuous remodelling. Endomembrane trafficking phragmoplast remodelling are tightly coupled, indicating existence active crosstalk. However, although many molecular regulators membrane...
Spatially directed cell division and expansion is important for plant growth morphogenesis relies on cooperation between the cytoskeleton secretory pathway. The phylogenetically conserved octameric complex exocyst mediates exocytotic vesicle tethering at plasma membrane. Unlike other subunits of land plants, core subunit SEC6 exists as a single paralog in Physcomitrium patens Arabidopsis thaliana genomes. (AtSEC6) loss-of-function (LOF) mutation causes male gametophytic lethality. Our...
Abstract Keyhole limpet hemocyanin (KLH)‐conjugated peptides are routinely used to raise polyclonal antibodies for biochemical or immunolocalization studies. Rats suitable producing antisera against plant antigens as they often lack non‐specific response towards materials. We attempted obtain rat derived from several proteins. However, most recognized the same background KLH‐related antigen (KRAP) in Arabidopsis and tobacco. characterized KRAP with respect size cellular localization examined...
Exocyst is a heterooctameric protein complex crucial for the tethering of secretory vesicles to plasma membrane during exocytosis. Compared other eukaryotes, exocyst subunit EXO70 represented by many isoforms in land plants whose cell biological and roles, as well modes regulation remain largely unknown. Here, we present data on phospho-regulation isoform EXO70C2, which previously identified putative negative regulator function pollen tube growth. A comprehensive phosphoproteomic analysis...
The exocyst complex is an octameric evolutionarily conserved tethering engaged in the regulation of polarized secretion eukaryotic cells. Here, we focus on systematic comparison two isoforms SEC15 subunit, SEC15a and SEC15b. We infer that gene duplication diversification occurred common ancestor seed plants (Spermatophytes). In Arabidopsis, represents main isoform male gametophyte, localizes to pollen tube tip at plasma membrane. Although tubes sec15a mutants are impaired, sporophytes show...