A5040919099- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Bacteriophages and microbial interactions
- Transgenic Plants and Applications
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- Polysaccharides and Plant Cell Walls
- Plant Disease Resistance and Genetics
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Autophagy in Disease and Therapy
- Microbial Community Ecology and Physiology
- Islanding Detection in Power Systems
- Microbial infections and disease research
- Mitochondrial Function and Pathology
- Insect Resistance and Genetics
- Plant responses to water stress
- Plant pathogens and resistance mechanisms
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
Charles University
2011-2024
Czech Academy of Sciences, Institute of Experimental Botany
2012-2024
Czech Academy of Sciences
1997-2013
Ghent University
2007
Vlaams Instituut voor Biotechnologie
2007
Czech Academy of Sciences, Institute of Molecular Genetics
1997
Plant pathogens are perceived by pattern recognition receptors, which activated upon binding to pathogen-associated molecular patterns (PAMPs). Ubiquitination and vesicle trafficking have been linked the regulation of immune signaling. However, little information exists about components involved in signaling mechanisms that regulate them. In this study, we identified Arabidopsis thaliana Exo70B2, a subunit exocyst complex mediates tethering during exocytosis, as target plant U-box–type...
Abstract The exocyst, an octameric tethering complex and effector of Rho Rab GTPases, facilitates polarized secretion in yeast animals. Recent evidence implicates three plant homologs exocyst subunits (SEC3, SEC8, EXO70A1) cell morphogenesis. Here, we provide genetic, biological, biochemical that these other predicted function together vivo Arabidopsis thaliana. Double mutants (sec5 exo70A1 sec8 exo70A1) show a synergistic defect etiolated hypocotyl elongation. Mutants SEC5, SEC6, SEC15a...
Autophagic transport to the vacuole represents an endomembrane trafficking route, which is widely used in plants, not only during stress situations, but also for biogenesis and developmental processes. Here we report a role autophagic membrane EXO70B1--one of 23 paralogs Arabidopsis EXO70 exocyst subunits. EXO70B1 positive compartments are internalized into central co-localize with autophagosomal marker ATG8f. This internalization boosted by induction autophagy. Loss function (LOF) mutations...
Recently, the octameric vesicle-tethering complex exocyst was found in plants and its importance for Arabidopsis morphogenesis demonstrated. Exo70 subunits plants, unlike yeasts mammals, are represented by a multigene family, comprising 23 members Arabidopsis. For Exo70B2 Exo70H1 paralogues, transcriptional up-regulation confirmed on treatment with an elicitor peptide, elf18, derived from bacterial elongation factor. Their ability to participate formation inferred interaction of both Exo70s...
Cell reproduction is a complex process involving whole cell structures and machineries in space time, resulting regulated distribution of endomembranes, organelles, genomes between daughter cells. Secretory pathways supported by the activity Golgi apparatus play crucial role cytokinesis plants. From onset phragmoplast initiation to maturation plate, delivery secretory vesicles necessary sustain successful separation. Tethering at plasma membrane mediated evolutionarily conserved octameric...
In land plants polar auxin transport is one of the substantial processes guiding whole plant polarity and morphogenesis. Directional fluxes are mediated by PIN efflux carriers, polarly localized at plasma membrane. The polarization exocytosis in yeast animals assisted exocyst: an octameric vesicle-tethering complex effector Rab Rho GTPases. Here we show that rootward compromised roots Arabidopsis thaliana loss-of-function mutants EXO70A1 exocyst subunit. recycling PIN1 PIN2 proteins from...
The exocyst complex, an effector of Rho and Rab GTPases, is believed to function as exocytotic vesicle tether at the plasma membrane before soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex formation. Exocyst subunits localize secretory-active regions membrane, exemplified by outer domain Arabidopsis root epidermal cells. Using variable-angle epifluorescence microscopy, we visualized dynamics this domain. colocalized in defined foci distinct from endocytic...
Polarized exocytosis is essential for many vital processes in eukaryotic cells, where secretory vesicles are targeted to distinct plasma membrane domains characterized by their specific lipid-protein composition. Heterooctameric protein complex exocyst facilitates the vesicle tethering a target and principal cell polarity regulator eukaryotes. The architecture molecular details of plant its recruitment have remained elusive. Here, we show that consists two modules formed SEC3-SEC5-SEC6-SEC8...
Summary The Arabidopsis thaliana genome expresses five evolutionarily conserved prohibitin (PHB) genes that are divided into type‐I ( AtPHB3 and AtPHB4 ) type‐II AtPHB1 , AtPHB2 AtPHB6 classes, based on their phylogenetic relationships with yeast PHB1 PHB2, respectively. Yeast animal PHBs reported to have diverse roles in the cell cycle, mitochondrial electron transport, aging apoptosis. All transcribed PHB primarily expressed both shoot root proliferative tissues, where they present...
Cortical microtubules (MTs) play a major role in the patterning of secondary cell wall (SCW) thickenings tracheary elements (TEs) by determining sites SCW deposition. The EXO70A1 subunit exocyst secretory vesicle tethering complex was implicated to be important for TE development via MT interaction. We investigated subcellular localization several subunits xylem Arabidopsis thaliana and analyzed functional significance exocyst-mediated trafficking development. Live imaging fluorescently...
The GOLVEN (GLV) gene family encode small secreted peptides involved in important plant developmental programs. Little is known about the factors required for production of mature bioactive GLV peptides. Through a genetic suppressor screen Arabidopsis thaliana, two related subtilase genes, AtSBT6.1 and AtSBT6.2, were identified that are necessary GLV1 activity. Root hypocotyl overexpression phenotypes suppressed by mutations either genes. Synthetic GLV-derived cleaved vitro affinity-purified...
The Arabidopsis thaliana pathogenesis-related 1 (PR1) is an important defense protein, so far it has only been detected in extracellular space and its subcellular sorting transport remain unexplained. Using a green fluorescent protein (GFP) tagged full length, as well C-terminus truncated version of PR1, we observed that when expressed ectopically Nicotiana benthamiana leaves, PR1 co-localizes partially with Golgi markers, much more prominently the late endosome (LE)/multivesicular body...
The pathogenesis-related 1 (PR1) proteins are members of the cross-kingdom conserved CAP superfamily (from Cysteine-rich secretory protein, Antigen 5, and PR1 proteins). mRNA expression is frequently used for biotic stress monitoring in plants; however, molecular mechanisms its cellular processing, localization, function still unknown. To analyse localization immunity features Arabidopsis thaliana PR1, we employed transient Nicotiana benthamiana tagged full-length construct, also disrupted...
Abstract In the reaction to non-adapted Blumeria graminis f. sp. hordei (Bgh), Arabidopsis thaliana leaf epidermal cells deposit cell wall reinforcements called papillae or seal fungal haustoria in encasements, both of which involve intensive exocytosis. A plant syntaxin, SYP121/PEN1, has been found be key importance for timely formation papillae, and vesicle tethering complex exocyst subunit EXO70B2 contribute their morphology. Here, we identify a specific role EXO70B2-containing membrane...
Selected beneficial Pseudomonas spp. strains have the ability to influence root architecture in Arabidopsis thaliana by inhibiting primary elongation and promoting lateral hair formation. A crucial role for auxin this long-term (1week), long-distance plant–microbe interaction has been demonstrated. seedlings were cultivated vitro on vertical plates inoculated with pathogenic syringae pv. maculicola (Psm) P. tomato DC3000 (Pst), as well Agrobacterium tumefaciens (Atu) Escherichia coli (Eco)....
Abstract Although angiosperm plants generally react to immunity elicitors like chitin or chitosan by the cell wall callose deposition, this response in particular types, especially upon treatment, is not fully understood. Here we show that growing root hairs (RHs) of Arabidopsis can respond a mild (0.001%) treatment deposition and deceleration RH growth. We demonstrate glucan synthase‐like 5/PMR4 vital for chitosan‐induced but growth inhibition. Upon higher concentration (0.01%) RHs do...
The exocyst heterooctameric vesicle-tethering complex is important for plant development, growth and immunity. Multiple paralogs exist most subunits of this complex; especially the membrane-interacting subunit EXO70 underwent extensive amplification in land plants, suggesting functional specialization. Despite specialization, Arabidopsis exo70 mutants are viable free developmental defects, probably as a consequence redundancy among isoforms. Our silico data-mining modelling analysis,...