A5081399608- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Plant responses to water stress
- Tissue Engineering and Regenerative Medicine
- Plant Parasitism and Resistance
- Genetic and Environmental Crop Studies
- Potato Plant Research
- Plant Physiology and Cultivation Studies
- Plant Genetic and Mutation Studies
- Soybean genetics and cultivation
- Plant Pathogenic Bacteria Studies
- Phytase and its Applications
- Agriculture, Plant Science, Crop Management
- Islanding Detection in Power Systems
Charles University
2015-2025
Institute of Plant Molecular Biology
2025
Czech Academy of Sciences
2013-2025
Institute of Science and Technology Austria
2022-2025
University of Regensburg
2020-2021
Czech Academy of Sciences, Institute of Experimental Botany
2013-2017
Autophagic transport to the vacuole represents an endomembrane trafficking route, which is widely used in plants, not only during stress situations, but also for biogenesis and developmental processes. Here we report a role autophagic membrane EXO70B1--one of 23 paralogs Arabidopsis EXO70 exocyst subunits. EXO70B1 positive compartments are internalized into central co-localize with autophagosomal marker ATG8f. This internalization boosted by induction autophagy. Loss function (LOF) mutations...
Recently, the octameric vesicle-tethering complex exocyst was found in plants and its importance for Arabidopsis morphogenesis demonstrated. Exo70 subunits plants, unlike yeasts mammals, are represented by a multigene family, comprising 23 members Arabidopsis. For Exo70B2 Exo70H1 paralogues, transcriptional up-regulation confirmed on treatment with an elicitor peptide, elf18, derived from bacterial elongation factor. Their ability to participate formation inferred interaction of both Exo70s...
Exocyst is an evolutionarily conserved vesicle tethering complex functioning especially in the last stage of exocytosis. Homologs its eight canonical subunits - Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84 were found also higher plants confirmed to form complexes vivo, participate cell growth including polarized expansion pollen tubes root hairs. Here we present results a phylogenetic study land plant exocyst encoded by selection completely sequenced genomes representing variety...
In land plants polar auxin transport is one of the substantial processes guiding whole plant polarity and morphogenesis. Directional fluxes are mediated by PIN efflux carriers, polarly localized at plasma membrane. The polarization exocytosis in yeast animals assisted exocyst: an octameric vesicle-tethering complex effector Rab Rho GTPases. Here we show that rootward compromised roots Arabidopsis thaliana loss-of-function mutants EXO70A1 exocyst subunit. recycling PIN1 PIN2 proteins from...
Arabidopsis (Arabidopsis thaliana) leaf trichomes are single-cell structures with a well-studied development, but little is understood about their function. Developmental studies focused mainly on the early shaping stages, and attention has been paid to maturation stage. We EXO70H4 exocyst subunit, one of most up-regulated genes in mature trichome. uncovered EXO70H4-dependent development secondary cell wall layer, highly autofluorescent callose rich, deposited only upper part The boundary...
Biogenesis of the plant secondary cell wall involves many important aspects, such as phenolic compound deposition and often silica encrustation. Previously, we demonstrated importance exocyst subunit EXO70H4 for biogenesis trichome wall, namely autofluorescent callose-rich layer. Here, reveal that EXO70H4-driven is constitutively active in mature trichome, but also can be activated elsewhere upon pathogen attack, giving this study a broader significance with an overlap into phytopathology....
• Polarized deposition of cell wall pectins is a key process in Arabidopsis thaliana myxospermous seed coat development. The exocyst, an octameric secretory vesicle tethering complex, has recently been shown to be involved the regulation polarity plants. Here, we used study participation exocyst complex polarized pectin delivery. We characterized amount pectinaceous mucilage and structure sec8 exo70A1 mutants. Using yeast two-hybrid screen, identified new interactor subunit Exo70A1, termed...
The exocyst is a conserved vesicle-tethering complex with principal roles in cell polarity and morphogenesis. Several studies point to its involvement polarized secretion during microbial pathogen defense. In this context, we have found an interaction between the Arabidopsis EXO70B1 subunit, protein which was previously associated both defense response autophagy, RPM1 INTERACTING PROTEIN 4 (RIN4), best studied member of NOI family known regulator plant pathways. Interestingly, fragments RIN4...
The exocyst, a eukaryotic tethering complex, coregulates targeted exocytosis as an effector of small GTPases in polarized cell growth. In land plants, several exocyst subunits are encoded by double or triple paralogs, culminating tens EXO70 paralogs. Out 23 Arabidopsis thaliana isoforms, we analyzed seven isoforms expressed pollen. Genetic and microscopic analyses single mutants EXO70A2, EXO70C1, EXO70C2, EXO70F1, EXO70H3, EXO70H5, EXO70H6 genes revealed that only loss-of-function EXO70C2...
Abstract Plant cells respond to a wide range of stimuli through intracellular calcium (Ca 2+ ) signaling. Cyclic nucleotide-gated channels (CNGCs) are major class plant Ca channels, with 20 homologs in Arabidopsis . These tetrameric plasma membrane (PM) proteins activated by diverse stimuli, such as phytohormones, extracellular damage and cell wall integrity signals. Here, we identify novel plant-specific proteins, Armadillo Repeat Only (ARO), essential regulators possibly all CNGC channels....
Abstract Reactive oxygen species (ROS) have been implicated repeatedly in multiple signaling processes plants but the underlying mechanisms and roles remain enigmatic. Here, we developed live imaging of apoplastic ROS at root surface. Different signals, including auxin, extracellular ATP RALF1 peptide, all induce cytosolic calcium transients bursts. Genetic optogenetic manipulations identified as necessary sufficient for bursts via activation NADPH oxidases RBOHC RBOHF. Apoplastic are not...
To identify novel genes engaged in plant epidermal development, we characterized the phenotypic variability of rosette leaf epidermis 310 sequenced Arabidopsis thaliana accessions, focusing on trichome shape and distribution, compositional characteristics cell wall, histologically detectable metal ion distribution. Some these traits correlated with cLimate parameters our accession's locations origin, suggesting environmental selection. A deposition pattern stomatal guard cells was observed...
Papaver somniferum (poppy) is a traditional component of Central and Eastern European cuisine an important oilseed crop in the region. The crucial thread for poppy stable yield pathogen infection. Thus, we need to understand defence mechanisms detail. first robust layer plant immunity, which plays role combat against pathogens, pattern-triggered immunity (PTI). Here, provide insights into PTI poppy. We selected four varieties used food industry. investigated response various peptide...
Plasma membrane (PM) lipid composition and domain organization are modulated by polarized exocytosis. Conversely, targeting of secretory vesicles at specific domains in the PM is carried out exocyst complexes, which contain EXO70 subunits that play a significant role final recognition target membrane. As we have shown previously, mature Arabidopsis trichome contains basal with thin cell wall an apical thick secondary wall, developed EXO70H4-dependent manner. These separated structure named...
Pollen development, pollen grain germination, and tube elongation are crucial biological processes in angiosperm plants that need precise regulation to deliver sperm cells ovules for fertilization. Highly polarized secretion at a growing tip requires the exocyst tethering complex responsible specific targeting of secretory vesicles plasma membrane. Here, we demonstrate Arabidopsis (Arabidopsis thaliana) EXO70A2 (At5g52340) is main EXO70 isoform male gametophyte, governing conventional...
The At-Hook Motif Nuclear Localized Protein (AHL) gene family encodes embryophyte-specific nuclear proteins with DNA binding activity. They modulate expression and affect various developmental processes in plants. We identify AHL18 (At3G60870) as a modulator of root system architecture growth. is involved regulation the length proliferation domain number dividing cells apical meristem thereby, cell production. Both primary growth lateral development respond according to transcription level....
Abstract In the reaction to non-adapted Blumeria graminis f. sp. hordei (Bgh), Arabidopsis thaliana leaf epidermal cells deposit cell wall reinforcements called papillae or seal fungal haustoria in encasements, both of which involve intensive exocytosis. A plant syntaxin, SYP121/PEN1, has been found be key importance for timely formation papillae, and vesicle tethering complex exocyst subunit EXO70B2 contribute their morphology. Here, we identify a specific role EXO70B2-containing membrane...
Abstract The major developmental signal in plants, auxin is perceived by TIR1/AFB receptors. It triggers transcriptional reprogramming via well-established canonical mechanism but also elusive rapid, non-transcriptional responses. Here we demonstrate that receptors have, next to recently identified adenylate cyclase, guanylate cyclase activity. Auxin perception activates independently the cAMP and cGMP production TIR1/AFBs vitro increases levels planta with a slow fast dynamics,...
Exocyst component of 70-kDa (EXO70) proteins are constituents the exocyst complex implicated in vesicle tethering during exocytosis. MILDEW RESISTANCE LOCUS O (MLO) plant-specific calcium channels and some MLO isoforms enable fungal powdery mildew pathogenesis. We here detected an unexpected phenotypic overlap Arabidopsis thaliana exo70H4 mlo2 mlo6 mlo12 triple mutant plants regarding biogenesis leaf trichome secondary cell walls. Biochemical Fourier transform infrared spectroscopic analyses...
Root gravitropic bending represents a fundamental aspect of terrestrial plant physiology. Gravity is perceived by sedimentation starch-rich plastids (statoliths) to the bottom central root cap cells. Following gravity perception, intercellular auxin transport redirected downwards leading an asymmetric accumulation at lower side causing inhibition cell expansion, ultimately resulting in bending. How gravity-induced statoliths repositioning translated into distribution remains unclear despite...