A5101940647- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Transgenic Plants and Applications
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Metabolism and Genetic Disorders
- Geochemistry and Elemental Analysis
- Bacteriophages and microbial interactions
- Pharmacogenetics and Drug Metabolism
- Plant Reproductive Biology
- Renal function and acid-base balance
- Nicotinic Acetylcholine Receptors Study
- Phytase and its Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Parasitism and Resistance
- Cellular transport and secretion
- Ion channel regulation and function
- Protein purification and stability
- Plant responses to water stress
- Viral gastroenteritis research and epidemiology
- Venomous Animal Envenomation and Studies
- Plant-Microbe Interactions and Immunity
Technical University of Munich
2014-2024
Sutro Biopharma (United States)
2011-2016
Kaiser Permanente South San Francisco Medical Center
2014
Washington University in St. Louis
1932
Jewish Hospital
1931
Engineering robust protein production and purification of correctly folded biotherapeutic proteins in cell-based systems is often challenging due to the requirements for maintaining complex cellular networks cell viability need develop associated downstream processes that reproducibly yield biopharmaceutical products with high product quality. Here, we present an alternative Escherichia coli-based open cell-free synthesis (OCFS) system optimized predictable high-yield folding at any scale...
Antibodies are well-established as therapeutics, and the preclinical clinical pipeline of these important biologics is growing rapidly. Consequently, there considerable interest in technologies to engineer manufacture them. Mammalian cell culture commonly used for production because eukaryotic expression systems have evolved complex efficient chaperone folding antibodies. However, given ease manipulability bacteria, antibody discovery efforts often employ bacterial despite their limitations...
Transport Protein Particle II (TRAPPII) is essential for exocytosis, endocytosis, protein sorting and cytokinesis. In spite of a considerable understanding its biological role, little information known about Arabidopsis TRAPPII complex topology molecular function. this study, independent proteomic approaches initiated with TRAPP components or Rab-A GTPase variants converge on the complex. We show that genome encodes full complement 13 TRAPPC subunits, including four previously unidentified...
Summary Cytokinesis, the partitioning of cytoplasm following nuclear division, requires extensive coordination between cell cycle cues, membrane trafficking and microtubule dynamics. Plant cytokinesis occurs within a transient compartment known as plate, to which vesicles are delivered by plant‐specific array, phragmoplast. While proteins required for known, how these coordinated with dynamics regulated cues remains unclear. Here, we document physical genetic interactions Transport Protein...
Research Article| January 01 1931 Notes on the Hagedorn-Jensen method for determination of blood-sugar Hildegarde Kramer; Kramer 1The Laboratory Jewish Hospital St Louis, Louis Search other works by this author on: This Site PubMed Google Scholar Alexander Steiner 1 Biochem J (1931) 25 (1): 161–165. https://doi.org/10.1042/bj0250161 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn Email Cite Get Permissions Citation...
ABSTRACT The trans-Golgi-network (TGN) has essential housekeeping functions in secretion, endocytosis and protein sorting, but also more specialized plant development. How the robustness of basal TGN function is ensured while are differentially regulated poorly understood. Here, we investigate two key regulators structure function, ECHIDNA Transport Protein Particle II (TRAPPII) tethering complex. An analysis physical, network genetic interactions suggests that communities implicated TRAPPII...
Plants often adapt to adverse or stress conditions via differential growth. The trans-Golgi network (TGN) has been implicated in responses, but it is not clear what capacity mediates adaptive growth decisions. In this study, we assess the role of TGN responses by exploring previously identified interactome Transport Protein Particle II (TRAPPII) complex required for structure and function. We physical genetic interactions between AtTRAPPII shaggy-like kinases (GSK3/AtSKs) provided vitro vivo...
Research Article| January 01 1932 The sugars of urine: chemical nature the fermentable sugar normal and starvation urine Edward Staunton West; West 1The Laboratory Biological Chemistry, Washington University School Medicine, Saint Louis, Missouri Search for other works by this author on: This Site PubMed Google Scholar Alexander Steiner Biochem J (1932) 26 (6): 1742–1749. https://doi.org/10.1042/bj0261742 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review...
Plants often adapt to adverse or stress conditions via differential growth. The trans-Golgi Network (TGN) has been implicated in responses, but it is not clear what capacity mediates adaptive growth decisions. In this study, we assess the role of TGN responses by exploring interactome Transport Protein Particle II (TRAPPII) complex, required for structure and function. We identified physical genetic interactions between TRAPPII shaggy-like kinases (GSK3/AtSKs). Kinase assays pharmacological...