Kim Büttgen

ORCID: 0009-0007-3009-7041
Publications
Citations
Views
---
Saved
---
About
Contact & Profiles
Research Areas
  • Plant Reproductive Biology
  • Polysaccharides and Plant Cell Walls
  • Plant Molecular Biology Research
  • Plant biochemistry and biosynthesis
  • Lipid metabolism and biosynthesis
  • Biotin and Related Studies

RWTH Aachen University
2022-2023

Exocyst component of 70-kDa (EXO70) proteins are constituents the exocyst complex implicated in vesicle tethering during exocytosis. MILDEW RESISTANCE LOCUS O (MLO) plant-specific calcium channels and some MLO isoforms enable fungal powdery mildew pathogenesis. We here detected an unexpected phenotypic overlap Arabidopsis thaliana exo70H4 mlo2 mlo6 mlo12 triple mutant plants regarding biogenesis leaf trichome secondary cell walls. Biochemical Fourier transform infrared spectroscopic analyses...

10.1093/plcell/koad319 article EN cc-by-nc-nd The Plant Cell 2023-12-20

Rosette leaf trichomes of Arabidopsis thaliana have been broadly used to study cell development, differentiation and, more recently, wall biogenesis. However, trichome-specific biochemical or -omics analyses require a proper separation from residual plant tissue. Thus, different strategies were proposed in the past for trichome isolation, which mostly rely on harsh conditions and suffer low yield, thereby limiting spectrum downstream analyses.To take trichome-leaf next level, we revised...

10.1186/s13007-021-00836-0 article EN cc-by Plant Methods 2022-01-28

Abstract Trichomes are fine outgrowths on the surface of aerial plant organs which play a role in protecting plants against water loss, UV radiation, and herbivore feeding. Throughout years, trichomes have become popular paradigm biological research. For example, rosette leaves reference Arabidopsis thaliana been used as model to investigate cell development, differentiation, and, more recently, wall biogenesis. State art ‐omics studies specific types or tissues often require physical...

10.1002/cpz1.541 article EN cc-by-nc-nd Current Protocols 2022-09-01

Abstract EXO70 proteins are essential constituents of the octameric exocyst complex implicated in vesicle tethering during exocytosis, while MLO plant-specific calcium channels which some isoforms play a key role fungal powdery mildew pathogenesis. We here detected by variety histochemical staining procedures an unexpected phenotypic overlap A. thaliana exo70H4 and mlo2 mlo6 mlo12 triple mutant plants regarding biogenesis leaf trichome secondary cell walls. Biochemical Fourier transform...

10.1101/2022.12.30.521597 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2022-12-30
Coming Soon ...