A5012882131- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant Pathogens and Resistance
- Macrophage Migration Inhibitory Factor
- Nuclear Receptors and Signaling
- Biotin and Related Studies
- Plant biochemistry and biosynthesis
- Galectins and Cancer Biology
- Plant Pathogens and Fungal Diseases
- Nematode management and characterization studies
- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Legume Nitrogen Fixing Symbiosis
- bioluminescence and chemiluminescence research
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Cellular transport and secretion
- Fungal and yeast genetics research
RWTH Aachen University
2014-2023
FH Aachen
2023
Summary Nonhost resistance ( NHR ) is the most prevalent form of plant immunity. In Arabidopsis, requires membrane‐localized ATP ‐binding cassette ABC transporter PENETRATION PEN 3. Upon perception pathogen‐associated molecular patterns, 3 becomes phosphorylated, suggestive regulation by post‐translational modification. Here, we investigated protein interaction network. We probed Arabidopsis microarray At PMA ‐5000 with N‐terminal cytoplasmic domain Several proteins identified to interact in...
Rosette leaf trichomes of Arabidopsis thaliana have been broadly used to study cell development, differentiation and, more recently, wall biogenesis. However, trichome-specific biochemical or -omics analyses require a proper separation from residual plant tissue. Thus, different strategies were proposed in the past for trichome isolation, which mostly rely on harsh conditions and suffer low yield, thereby limiting spectrum downstream analyses.To take trichome-leaf next level, we revised...
Reversible protein phosphorylation is a widespread posttranslational modification that plays key role in eukaryotic signal transduction. Due to the dynamics of abundance, low stoichiometry and transient nature phosphorylation, detection accurate quantification substrate by kinases remains challenge phosphoproteome research. Here, we combine tandem metal-oxide affinity chromatography (tandemMOAC) with stable isotope 15N metabolic labeling for measurement abundant, transiently phosphorylated...
Mitogen-activated protein kinase (MPK) cascades are important to cellular signaling in eukaryotes. They regulate growth, development and the response environmental challenges. MPK function via reversible phosphorylation of cascade components, MEKK, MEK, MPK, but also by substrate phosphorylation. Using mass spectrometry, we previously identified many vivo MPK3 MPK6 substrates Arabidopsis thaliana, disclosed their sites.We verified several our MPK3/6 using a nonradioactive vitro labeling...
Human macrophage migration inhibitory factor (MIF) is an atypical chemokine implicated in intercellular signaling and innate immunity. MIF orthologs (MIF/D-DT-like proteins, MDLs) are present throughout the plant kingdom, but remain experimentally unexplored these organisms. Here, we provide planta characterization functional analysis of three-member gene/protein MDL family Arabidopsis thaliana. Subcellular localization experiments indicated a nucleo-cytoplasmic distribution MDL1 MDL2, while...
SUMMARY Loss‐of‐function alleles of plant MLO genes confer broad‐spectrum resistance to powdery mildews in many eudicot and monocot species. Although barley ( Hordeum vulgare ) mlo mutants have been used agriculture for more than 40 years, understanding the molecular principles underlying this type disease remains fragmentary. Forward genetic screens revealed mutations two Required Ror that partially impair immunity conferred by mutants. While Ror2 encodes a soluble N...
Mammalian macrophage migration inhibitory factor (MIF) and its paralog, D-dopachrome tautomerase, are multifunctional inflammatory cytokines. Plants have orthologous MIF tautomerase–like (MDL) proteins that mimic some of the effects on immune cells in vitro. We explored structural functional similarities between three Arabidopsis thaliana MDLs MIF. X-ray crystallography revealed high similarity MDL homotrimers suggested a potential explanation for lack tautomerase activity MDLs. MDL1 MDL2...
Abstract The purification of low-abundance protein complexes and detection in vivo protein–protein interactions complex biological samples remains a challenging task. Here, we devised crosslinking tandem affinity coupled to mass spectrometry (XL–TAP–MS), quantitative proteomics approach for analyzing affinity-purified, crosslinked from plant tissues. We exemplarily applied XL–TAP–MS study the MKK2–Mitogen-activated kinase (MPK4) signaling module Arabidopsis thaliana. A tag consisting an...
Abstract Mildew resistance locus o (MLO) proteins are heptahelical integral membrane of which some isoforms act as susceptibility factors for the fungal powdery mildew pathogen. In many angiosperm plant species, loss-of-function mlo mutants confer durable broad-spectrum against disease. Barley Mlo is known to interact via a cytosolic carboxyl-terminal domain with intracellular calcium sensor calmodulin (CAM) in calcium-dependent manner. Site-directed mutagenesis has revealed key amino acid...
Abstract Vertebrates have developed effective immune mechanisms to fight microbial attacks, relying on a sophisticated network of innate and adaptive responses, circulatory system, numerous orchestrating soluble mediators such as cytokines. Mammalian macrophage migration inhibitory factor (MIF) its paralog D-dopachrome tautomerase (D-DT/MIF-2) are multifunctional inflammatory cytokines with chemokine-like properties that modulate immunity. Plants possess orthologous MIF/D-DT-like (MDL)...
Mildew resistance locus o (MLO) proteins are heptahelical integral membrane of which some isoforms act as susceptibility factors for the powdery mildew pathogen. In many angiosperm plant species, loss-of-function mlo mutants confer durable broad-spectrum against fungal disease. Barley Mlo is known to interact via a cytosolic carboxyl-terminal domain with intracellular calcium sensor calmodulin (CAM) in calcium-dependent manner. Site-directed mutagenesis has revealed key amino acid residues...