A5041368131- Liver Disease Diagnosis and Treatment
- Macrophage Migration Inhibitory Factor
- Sirtuins and Resveratrol in Medicine
- Liver Disease and Transplantation
- Nuclear Receptors and Signaling
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Parkinson's Disease Mechanisms and Treatments
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Amyotrophic Lateral Sclerosis Research
- Biochemical effects in animals
- Drug-Induced Hepatotoxicity and Protection
- Phytochemicals and Medicinal Plants
- Lymphoma Diagnosis and Treatment
- Hepatitis Viruses Studies and Epidemiology
- Algal biology and biofuel production
- Urinary Tract Infections Management
- Livestock and Poultry Management
- Multiple Myeloma Research and Treatments
- Autophagy in Disease and Therapy
- Inorganic Fluorides and Related Compounds
- Bacterial Identification and Susceptibility Testing
- Nanoparticles: synthesis and applications
- Neurogenetic and Muscular Disorders Research
Sarojini Naidu Medical College
2015-2024
National Institute of Mental Health and Neurosciences
1983-2024
Yale University
2021-2024
Indian Institute of Technology Tirupati
2024
Government Medical College
2023
University of New Haven
2023
Institute of Medical Sciences
2021
Karnataka State Open University
2021
Aeronautical Development Establishment
2021
Madras Diabetes Research Foundation
2000-2019
Effect of thermal aging and chemical treatment on the physical properties coir fiber was investigated. Coir fibers were treated with sodium hydroxide glutaraldehyde for 2 h. The influence alkali aldehyde tensile strength elongation at break studied in detail. Enhancement observed up to five days 50°C further decreased. Thermal cross linking cellulose present may be reason increase degradation due chain scission reduced strength. Sodium-hydroxide-treated samples showed an reduction break....
Cu/Zn superoxide dismutase-1 (SOD1) mutations are causative for a subset of amyotrophic lateral sclerosis (ALS) cases. These lead to structural instability, aggregation and ultimately motor neuron death. We have determined crystal structures SOD1 in complex with naphthalene-catechol-linked compound which binds low micro-molar affinity site important oxidative damage-induced aggregation. Trp32 oxidation is indeed significantly inhibited by ligand binding. Our work shows how linking can be...
Abstract Background Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine that has been implicated in multiple inflammatory and non-inflammatory diseases, including liver injury induced by acetaminophen (APAP) overdose. Multiple small molecule inhibitors of MIF have described, the clinically available anti-rheumatic drug T-614 (iguratimod); however, this drug’s mode inhibition not fully investigated. Methods We conducted vitro testing kinetic analysis protein crystallography...
Human SIRT1 is a class III histone deacetylase (HDAC) family protein. As the overexpression of hSIRT1 leads to cancer, inhibiting its HDAC function may be better strategy for treatment cancer. Till now, only few reported inhibitor compounds have reached stage animal studies; hence, identifying high efficacy inhibitors essential.The main objective study obtain new by rational structure-based method.We performed virtual screening using AutoDock Vina domain against Drug- Bank library containing...
Background: Vitiligo is a common skin disorder causing depigmented macules that can impair patient's quality of life. There are number treatment modalities available but at the present, there no studies compairing betamethasone pulse therapy versus azathioprine daily in vitiligo. Objectives: To compare safety and efficacy Azathioprine Betamethasone Materials Methods: We included two vitiligo patient groups. namely group A B Patients were given oral to be 50mg twice those second B, as single...
Formation of Cu, Zn superoxide dismutase 1 (SOD1) protein inclusions within motor neurons is one the principal characteristics SOD1-related amyotrophic lateral sclerosis (ALS). A hypothesis as to nature SOD1 aggregation implicates oxidative damage a solvent-exposed tryptophan causative. Here, we chart discovery phenanthridinone based compound (Lig9) from NCI Diversity Set III by rational methods in silico screening and crystallographic validation. The crystal structure complex with SOD1,...
The macrophage migration inhibitory factor (MIF) family of cytokines contains multiple ligand-binding sites and mediates immunomodulatory processes through an undefined mechanism(s). Previously, we reported a dynamic relay connecting the MIF catalytic site to allosteric at its solvent channel. Despite structural functional similarity, homolog D-dopachrome tautomerase (also called MIF-2) has low sequence identity (35%), prompting question whether this regulatory network is conserved. Here,...
Abstract Dual-specificity mitogen-activated protein kinase (MAPK) phosphatases (MKPs) directly dephosphorylate and inactivate the MAPKs. Although catalytic mechanism of dephosphorylation MAPKs by MKPs is established, a complete molecular picture regulatory interplay between still remains to be fully explored. Here, we sought define MKP5 regulation through an allosteric site within its domain. We demonstrate using crystallographic NMR spectroscopy approaches that residue Y435 required...
Bromodomain containing proteins recognize the level of histone acetylation and regulate epigenetically controlled processes like gene transcription chromatin modification. The BET (bromodomain extra-terminal) family proteins, which are transcriptional co-regulators, have been implicated in pathogenesis cancer, neurodegenerative disorders, defects embryonic stem cell differentiation. Inhibitors selectively targeting bromodomains can pave path for new drug discovery against several forms major...
Macrophage migration inhibitory factor (MIF) is an inflammatory protein with various non-overlapping functions. It not only conserved in mammals, but it found parasites, fish, and plants. Human MIF a homotrimer enzymatic cavity between two subunits Pro1 as catalytic base, activates the receptors CD74, CXCR2, CXCR4, has functional interactions cytosol, reported to be nuclease. There solvent channel down its 3-fold axis recently identified gating residue allosteric site important for...
Mammalian macrophage migration inhibitory factor (MIF) and its paralog, D-dopachrome tautomerase, are multifunctional inflammatory cytokines. Plants have orthologous MIF tautomerase–like (MDL) proteins that mimic some of the effects on immune cells in vitro. We explored structural functional similarities between three Arabidopsis thaliana MDLs MIF. X-ray crystallography revealed high similarity MDL homotrimers suggested a potential explanation for lack tautomerase activity MDLs. MDL1 MDL2...