A5019905622- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
- Galectins and Cancer Biology
- Extracellular vesicles in disease
- Toxin Mechanisms and Immunotoxins
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Macrophage Migration Inhibitory Factor
- Cancer-related molecular mechanisms research
- Nuclear Receptors and Signaling
- Plant Virus Research Studies
- Viral Infections and Vectors
- MicroRNA in disease regulation
- Plant Pathogens and Resistance
- Plant Reproductive Biology
- Plant pathogens and resistance mechanisms
- Plant Genetic and Mutation Studies
- Wheat and Barley Genetics and Pathology
- Circular RNAs in diseases
RWTH Aachen University
2016-2024
Wheat is one of the most widely grown cereal crops in world and an important food grain source for humans. However, wheat yields can be reduced by many abiotic biotic stress factors, including powdery mildew disease caused Blumeria graminis f.sp. tritici (Bgt). Generating resistant varieties thus a major effort plant breeding. Here, we took advantage non-transgenic Targeting Induced Lesions IN Genomes (TILLING) technology to select partial loss-of-function alleles TaMlo, orthologue barley...
The biotrophic fungal pathogen Blumeria graminis causes the powdery mildew disease of cereals and grasses. We present first crystal structure a B. effector pathogenicity (CSEP0064/BEC1054), demonstrating it has ribonuclease (RNase)-like fold. This is part group RNase-like proteins (termed RALPHs) which comprise largest set secreted candidates within genomes. Their exceptional abundance suggests they play crucial functions during pathogenesis. show that transgenic expression RALPH...
The establishment of host-microbe interactions requires molecular communication between both partners, which may involve the mutual transfer noncoding small RNAs. Previous evidence suggests that this is also true for powdery mildew disease in barley, caused by fungal pathogen Blumeria hordei. However, previous studies lacked spatial resolution regarding accumulation RNAs upon host infection B. Here, we analysed site-specific RNA repertoires context barley-B. hordei interaction. To end,...
Human macrophage migration inhibitory factor (MIF) is an atypical chemokine implicated in intercellular signaling and innate immunity. MIF orthologs (MIF/D-DT-like proteins, MDLs) are present throughout the plant kingdom, but remain experimentally unexplored these organisms. Here, we provide planta characterization functional analysis of three-member gene/protein MDL family Arabidopsis thaliana. Subcellular localization experiments indicated a nucleo-cytoplasmic distribution MDL1 MDL2, while...
Abstract The mutual exchange of extracellular vesicles across kingdom borders is a feature many plant-microbe interactions. occurrence and cargos has been studied in several instances, but their dynamics the course infection have remained elusive. Here we used two different procedures, differential high-speed centrifugation polymer-based enrichment, to collect from apoplastic wash fluid barley ( Hordeum vulgare ) leaves challenged by its fungal powdery mildew pathogen, Blumeria hordei . Both...
Abstract The establishment of host-microbe interactions requires molecular communication between both partners, which involves the mutual transfer noncoding small RNAs. Previous evidence suggests that this is also true for barley powdery mildew disease, caused by fungal pathogen Blumeria hordei . However, previous studies lacked spatial resolution regarding accumulation RNAs upon host infection B. Here, we analysed site-specific RNA repertoires in context barley- interaction. To end,...
ABSTRACT RNA interference (RNAi) is a crucial mechanism that can contribute to immunity against infectious microbes through the action of DICER-LIKE (DCL) and ARGONAUTE (AGO) proteins. In case fungal pathogen Botrytis cinerea oomycete Hyaloperonospora arabidopsidis , plant DCL AGO proteins have proven roles as negative regulators immunity, suggesting functional specialization these To address this aspect in broader taxonomic context, we characterized colonization pattern an informative set...
Abstract In recent years, extracellular vesicles (EVs) have emerged as novel key players in plant–microbe interactions. While it is immensely useful to draw on the established “minimal information for studies of vesicles” (MISEV) guidelines and precedents mammalian systems, working with plants their associated microbes poses specific challenges. To navigate researchers through these obstacles, we offer detailed step‐by‐step suggestions those embarking EV research context The advice based...
Abstract RNA interference (RNAi) is a crucial mechanism in immunity against infectious microbes through the action of DICER‐LIKE (DCL) and ARGONAUTE (AGO) proteins. In case taxonomically diverse fungal pathogen Botrytis cinerea oomycete Hyaloperonospora arabidopsidis , plant DCL AGO proteins have proven roles as negative regulators immunity, suggesting functional specialization these To address this aspect broader taxonomic context, we characterized colonization pattern an informative set...
ABSTRACT The biotrophic fungal pathogen Blumeria graminis causes the powdery mildew disease of cereals and grasses. Proteins with a predicted ribonuclease (RNase)-like fold (termed RALPHs) comprise largest set secreted effector candidates within B. f. sp. hordei genome. Their exceptional abundance suggests they play crucial functions during pathogenesis. We show that transgenic expression RALPH CSEP0064/BEC1054 increases susceptibility to infection in monocotyledenous dicotyledonous plants....