A5051528092- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- Light effects on plants
- Cellular transport and secretion
- Plant Parasitism and Resistance
- Plant and fungal interactions
- Plant Diversity and Evolution
- Toxin Mechanisms and Immunotoxins
- Peptidase Inhibition and Analysis
- Biofuel production and bioconversion
- Polysaccharides Composition and Applications
- Polymer-Based Agricultural Enhancements
Institute of Science and Technology Austria
2016-2024
VIB-UGent Center for Plant Systems Biology
2021-2024
Ghent University
2021-2024
Charles University
2015-2021
Plants, like other multicellular organisms, survive through a delicate balance between growth and defense against pathogens. Salicylic acid (SA) is major signal in plants, the perception mechanism as well downstream signaling activating immune response are known. Here, we identify parallel SA that mediates attenuation. directly binds to A subunits of protein phosphatase 2A (PP2A), inhibiting activity this complex. Among PP2A targets, PIN2 auxin transporter hyperphosphorylated SA, leading...
Arabidopsis (Arabidopsis thaliana) leaf trichomes are single-cell structures with a well-studied development, but little is understood about their function. Developmental studies focused mainly on the early shaping stages, and attention has been paid to maturation stage. We EXO70H4 exocyst subunit, one of most up-regulated genes in mature trichome. uncovered EXO70H4-dependent development secondary cell wall layer, highly autofluorescent callose rich, deposited only upper part The boundary...
Significance This study identifies and outlines a nontranscriptional branch of the canonical GA signaling pathway that redirects protein traffic from vacuolar degradation route to plasma membrane. As result, amount receptors transporters, such as PIN transporters for plant hormone auxin, is functionally regulated at cell surface. The identified branching occurs level DELLA proteins that, besides transcriptional regulation, also target microtubule (MT) network trafficking. In this work, we...
Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which cargoes and lipids are internalized from the plasma membrane into vesicles coated with clathrin adaptor proteins. CME essential for many developmental physiological processes plants, but its underlying mechanism not well characterized compared that yeast animal systems. Here, we searched new factors involved Arabidopsis thaliana by performing tandem affinity purification of proteins interact light chain, principal...
Abstract Plant root architecture dynamically adapts to various environmental conditions, such as salt‐containing soil. The phytohormone abscisic acid (ABA) is involved among others also in these developmental adaptations, but the underlying molecular mechanism remains elusive. Here, a novel branch of ABA signaling pathway Arabidopsis involving PYR/PYL/RCAR (abbreviated PYLs) receptor‐protein phosphatase 2A (PP2A) complex that acts parallel canonical PYLs‐protein 2C (PP2C) identified....
Auxin is a major plant growth regulator, but current models on auxin perception and signaling cannot explain the whole plethora of effects, in particular those associated with rapid responses. A possible candidate for component additional mechanisms AUXIN BINDING PROTEIN 1 (ABP1), whose function planta remains unclear. Here we combined expression analysis gain- loss-of-function approaches to analyze role ABP1 development. shows broad largely overlapping with, not regulated by,...
Polar subcellular localization of the PIN exporters phytohormone auxin is a key determinant directional, intercellular transport and thus central topic both plant cell developmental biology. Arabidopsis mutants lacking PID, kinase that phosphorylates PINs, or MAB4/MEL proteins unknown molecular function display polarity defects phenocopy pin mutants, but mechanistic insights into how these factors convey are missing. Here, by combining protein biochemistry with quantitative live-cell...
Abstract The molecular mechanisms guiding oriented cell divisions in the root vascular tissues of Arabidopsis thaliana are still poorly characterised. By overlapping bulk and single‐cell transcriptomic datasets, we unveiled TETRASPANIN1 (TET1) as a putative regulator this process. TET1 is expressed cells, loss‐of‐function mutants contain fewer files. We further generated characterised CRISPR deletion mutant showed, unlike previously described mutants, that full knock out additionally missing...
Abstract Directionality in the intercellular transport of plant hormone auxin is determined by polar plasma membrane localization PIN-FORMED (PIN) proteins. However, apart from PIN phosphorylation at conserved motifs, no further determinants explicitly controlling sorting decisions have been identified. Here we present Arabidopsis WAVY GROWTH 3 (WAV3) and closely related RING-finger E3 ubiquitin ligases, whose loss-of-function mutants show a striking apical-to-basal polarity switch PIN2 root...
Transcriptional networks are crucial to integrate various internal and external signals into optimal responses during plant growth development. In Arabidopsis thaliana, primary root vasculature patterning proliferation controlled by a network centred around the basic Helix-Loop-Helix transcription factor complex, formed TARGET OF MONOPTEROS 5 (TMO5) LONESOME HIGHWAY (LHW), which control cell division orientation modulating cytokinin response other downstream factors. Despite recent progress,...
<ns4:p>The Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in plants. Since decades ago, it has been prime receptor candidate for plant hormone auxin with a plethora described functions signaling and development. The developmental importance ABP1 recently questioned by identification <ns4:italic>Arabidopsis thaliana abp1</ns4:italic> knock-out alleles that show no obvious phenotypes under normal growth conditions. In this study, we examined contradiction between...
Cell polarity is crucial for the coordinated development of all multicellular organisms. In plants, this exemplified by PIN-FORMED (PIN) efflux carriers phytohormone auxin: The polar subcellular localization PINs instructive to directional intercellular auxin transport, and thus a plethora auxin-regulated growth developmental processes. Despite its importance, regulation PIN remains poorly understood. Here, we have employed advanced live-cell imaging techniques study roles microtubules actin...
The intercellular transport of auxin is driven by PIN-formed (PIN) efflux carriers. PINs are localized at the plasma membrane (PM) and on constitutively recycling endomembrane vesicles. Therefore, can mediate either direct translocation across PM or pumping into secretory vesicles (SVs), leading to its release upon fusion with PM. Which these two mechanisms dominates a matter debate. Here, we addressed issue mathematical modeling approach. We demonstrate that efficiency depends SV size,...
The widely used non-steroidal anti-inflammatory drugs (NSAIDs) are derivatives of the phytohormone salicylic acid (SA). SA is well known to regulate plant immunity and development, whereas there have been few reports focusing on effects NSAIDs in plants. Our studies here reveal that exhibit largely overlapping physiological activities model Arabidopsis. NSAID treatments lead shorter agravitropic primary roots inhibited lateral root organogenesis. Notably, addition SA-like action, which...
Intercellular transport of auxin is driven by PIN-formed (PIN) proteins. PINs are localized at the plasma membrane (PM) and on constitutively recycling endomembrane vesicles. Therefore, can mediate either direct translocation across PM or pumping it into secretory vesicles (SVs), leading to its release upon fusion with PM. Which these two mechanisms dominates a matter debate. Here we addressed issue mathematical modeling approach. We demonstrate that efficiency depends SV size, half-life PM,...
Indole-3-acetic acid (IAA), the most abundant endogenous auxin is transported in plants a polar fashion by PIN-FORMED (PIN) transporters and controls virtually all plant growth developmental processes. Canonical PINs possess long largely disordered cytosolic loop domain which shorter non-canonical PINs. Auxin transport canonical activated phosphorylation kinases. While structure of transmembrane domains these was recently solved, their properties remained poorly characterized particularly...