A5020503247- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Light effects on plants
- Polysaccharides and Plant Cell Walls
BOKU University
2017-2024
Ghent University
2023-2024
VIB-UGent Center for Plant Systems Biology
2023-2024
Abstract Arabidopsis PIN2 protein directs transport of the phytohormone auxin from root tip into elongation zone. Variation in hormone transport, which depends on a delicate interplay between sorting to and polar plasma membrane domains, determines growth. By employing constitutively degraded version PIN2, we identify brassinolides as antagonists endocytosis. This response does not require de novo synthesis, but involves early events canonical brassinolide signaling. Brassinolide-controlled...
Protein abundance and localization at the plasma membrane (PM) shapes plant development mediates adaptation to changing environmental conditions. It is regulated by ubiquitination, a post-translational modification crucial for proper sorting of endocytosed PM proteins vacuole subsequent degradation. To understand significance variety roles played this reversible modification, function ubiquitin receptors, which translate signature into cellular response, needs be elucidated. In study, we...
Abstract The molecular mechanisms guiding oriented cell divisions in the root vascular tissues of Arabidopsis thaliana are still poorly characterised. By overlapping bulk and single‐cell transcriptomic datasets, we unveiled TETRASPANIN1 (TET1) as a putative regulator this process. TET1 is expressed cells, loss‐of‐function mutants contain fewer files. We further generated characterised CRISPR deletion mutant showed, unlike previously described mutants, that full knock out additionally missing...
Abstract Directionality in the intercellular transport of plant hormone auxin is determined by polar plasma membrane localization PIN-FORMED (PIN) proteins. However, apart from PIN phosphorylation at conserved motifs, no further determinants explicitly controlling sorting decisions have been identified. Here we present Arabidopsis WAVY GROWTH 3 (WAV3) and closely related RING-finger E3 ubiquitin ligases, whose loss-of-function mutants show a striking apical-to-basal polarity switch PIN2 root...
Coordination of plant development requires modulation growth responses that are under control the phytohormone auxin. PIN-FORMED plasma membrane proteins, involved in intercellular transport regulator, key to transmission such auxin signals and subject multilevel surveillance mechanisms, including reversible post-translational modifications. Apart from well-studied PIN protein modifications, namely phosphorylation ubiquitylation, no further modifications have been described so far. Here, we...
Intracellular sorting and the abundance of sessile plant plasma membrane proteins are imperative for sensing responding to environmental inputs. A key determinant inducing adjustments in protein localization hence functionality is their reversible covalent modification by small modifier ubiquitin, which example responsible guiding from endosomal compartments. This mode control requires catalytic activity E3 ubiquitin ligases, amongst members RING DOMAIN LIGASE (RGLG) family have been...
Abstract This study determines the functional role of plant ultraviolet‐B radiation (UV‐B) photoreceptor, UV RESISTANCE LOCUS 8 (UVR8) under natural conditions using a large‐scale ‘synchronized‐genetic‐perturbation‐field‐experiment’. Laboratory experiments have demonstrated for UVR8 in UV‐B responses but do not reflect complexity outdoor where ‘genotype × environment’ interactions can mask laboratory‐observed responses. Arabidopsis thaliana knockout mutant, uvr8‐7 , and corresponding...
Abstract The molecular mechanisms guiding oriented cell divisions in the root vascular tissues of Arabidopsis thaliana are still poorly characterized. By overlapping bulk and single-cell transcriptomic datasets, we unveiled TETRASPANIN1 (TET1) as a putative regulator this process. TET1 is expressed cells loss-of-function mutants contain fewer files. We further generated characterized CRISPR deletion mutant show, unlike previously described mutants, that full knock out additionally missing...