A5015178107- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Pathogenic Bacteria Studies
- Photosynthetic Processes and Mechanisms
- Plant pathogens and resistance mechanisms
- Plant Pathogens and Fungal Diseases
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- Caveolin-1 and cellular processes
- Mycorrhizal Fungi and Plant Interactions
- Lipid metabolism and biosynthesis
- Plant tissue culture and regeneration
- Renewable energy and sustainable power systems
- Aluminum toxicity and tolerance in plants and animals
- Plant Gene Expression Analysis
- Bacterial Infections and Vaccines
- Light effects on plants
- Plant Surface Properties and Treatments
- Philosophy, Science, and History
- Plant Micronutrient Interactions and Effects
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
University of South Bohemia in České Budějovice
2022-2025
University of Chemistry and Technology, Prague
2014-2023
Ludwig-Maximilians-Universität München
2019-2023
Czech Academy of Sciences, Institute of Experimental Botany
2013-2021
Vienna Biocenter
2021
Czech Academy of Sciences
2019
Institut d'écologie et des sciences de l'environnement de Paris
2019
Charles University
2018
Pathological Society
2014-2017
Institute of Chemical Technology
2013-2014
Recognition of pathogen-associated molecular patterns (PAMPs) is crucial for plant defence against pathogen attack. The best characterized PAMP flg22, a 22 amino acid conserved peptide from flagellin protein. In Arabidopsis thaliana, flg22 recognized by the sensing 2 (FLS2) receptor. this study, we focused on biotic stress responses triggered after exposure to temporary heat (HS). It important study reactions plants multiple conditions because are often exposed simultaneously combination...
Summary Phospholipids have recently been found to be integral elements of hormone signalling pathways. An Arabidopsis thaliana double mutant in two type III phosphatidylinositol‐4‐kinases ( PI 4Ks), pi4k β1β2 , displays a stunted rosette growth. The causal link between 4K activity and growth is unknown. Using microarray analysis, quantitative reverse transcription polymerase chain reaction (RT‐ qPCR ) multiple phytohormone analysis by LC‐MS we investigated the mechanism responsible for...
Being natural plant antimicrobials, saponins have potential for use as biopesticides. Nevertheless, their activity in plant–pathogen interaction is poorly understood. We performed a comparative study of saponins' antifungal activities on important crop pathogens based effective dose (EC50) values. Among those tested, aescin showed itself to be the strongest agent. The effect could reversed by ergosterol, thus suggesting that interferes with fungal sterols. tested two different pathosystems:...
Abstract Plant cells respond to a wide range of stimuli through intracellular calcium (Ca 2+ ) signaling. Cyclic nucleotide-gated channels (CNGCs) are major class plant Ca channels, with 20 homologs in Arabidopsis . These tetrameric plasma membrane (PM) proteins activated by diverse stimuli, such as phytohormones, extracellular damage and cell wall integrity signals. Here, we identify novel plant-specific proteins, Armadillo Repeat Only (ARO), essential regulators possibly all CNGC channels....
Papaver somniferum (poppy) is a traditional component of Central and Eastern European cuisine an important oilseed crop in the region. The crucial thread for poppy stable yield pathogen infection. Thus, we need to understand defence mechanisms detail. first robust layer plant immunity, which plays role combat against pathogens, pattern-triggered immunity (PTI). Here, provide insights into PTI poppy. We selected four varieties used food industry. investigated response various peptide...
The integrity of the actin cytoskeleton is essential for plant immune signalling. Consequently, it generally assumed that disruption reduces resistance to pathogen attack. Here, we demonstrate depolymerization induced a dramatic increase in salicylic acid (SA) levels Arabidopsis thaliana. Transcriptomic analysis showed SA pathway was activated due action isochorismate synthase (ICS). effect also confirmed Brassica napus. This raises question whether could, under particular conditions, lead...
Vesiculation is a process employed by Gram-negative bacteria to release extracellular vesicles (EVs) into the environment. EVs from pathogenic play functions in host immune modulation, elimination of defenses, and acquisition nutrients host. Here, we observed EV production bacterial speck disease causal agent, Pseudomonas syringae pv. tomato (Pto) DC3000, as outer membrane vesicle release. Mass spectrometry identified 369 proteins enriched Pto DC3000 EVs. The samples contained known...
The non-specific phospholipase C (NPC) is a new member of the plant family that reacts to abiotic environmental stresses, such as phosphate deficiency, high salinity, heat and aluminium toxicity, involved in root development, silicon distribution brassinolide signalling. Six NPC genes (NPC1–NPC6) are found Arabidopsis genome. NPC2 isoform has not been experimentally characterized so far. was cloned heterologously expressed Escherichia coli. enzyme activity determined using fluorescent...
Selected beneficial Pseudomonas spp. strains have the ability to influence root architecture in Arabidopsis thaliana by inhibiting primary elongation and promoting lateral hair formation. A crucial role for auxin this long-term (1week), long-distance plant–microbe interaction has been demonstrated. seedlings were cultivated vitro on vertical plates inoculated with pathogenic syringae pv. maculicola (Psm) P. tomato DC3000 (Pst), as well Agrobacterium tumefaciens (Atu) Escherichia coli (Eco)....
Phytohormone salicylic acid (SA) is a crucial component of plant induced defense against biotrophic pathogens. Although the key players SA pathway are known, there still gaps in our understanding molecular mechanism and regulation particular steps. In previous research we showed Arabidopsis suspension cells that n-butanol, which specifically modulates phospholipase D activity, significantly supresses transcription pathogenesis related (PR1) gene generally accepted as marker. presented study,...
Arabidopsis flotillin 2 (At5g25260) belongs to the group of plant flotillins, which are not well characterized. In contrast, metazoan flotillins known as plasma membrane proteins associated with microdomains that act a signaling hub. The similarity and whose functions most likely consist affecting other via protein-protein interactions, determines necessity detecting their interacting partners in plants. Nevertheless, identifying form complexes on is challenging task due low abundance...
The phytohormone salicylic acid (SA) has a crucial role in plant physiology. Its is best described the context of response to pathogen attack. During infection, SA rapidly accumulated throughout green tissues and important for both local systemic defences. However, some genetic/metabolic variations can also result overaccumulation plants, even basal conditions. To date, more than forty Arabidopsis thaliana mutants have been as having enhanced endogenous levels or constitutively activated...
The actin cytoskeleton is widely involved in plant immune responses. majority of studies show that chemical disruption the increases susceptibility to pathogen infection. Similarly, several pathogens have adopted this as a virulence strategy and produce effectors affect integrity. Such either exhibit actin-depolymerizing activity themselves or prevent polymerization. Is it thus possible for plants recognize actin's status launch counterattack? Recently we showed depolymerization filaments...
The use of light emitting diodes (LEDs) brings several key advantages over existing illumination technologies for indoor plant cultivation. Among these are that LEDs have predicted lifetimes from 50-100.000 hours without significant drops in efficiency and energy consumption is much lower compared to traditional fluorescent tubes. Recent advances allow be used with customized wavelengths growth. However, most LED growth systems mixtures chips narrow frequently they not compatible...
We have recently shown that an Arabidopsis thaliana double mutant of type III phosphatidylinositol-4-kinases (PI4Ks), pi4kβ1β2, constitutively accumulated a high level salicylic acid (SA). By crossing this pi4kβ1β2 with mutants impaired in SA synthesis (such as sid2 isochorismate synthase) or transduction, we demonstrated the was responsible for dwarfism phenotype mutant. Here aimed to distinguish between SA-dependent and SA-independent effects triggered by deficiency PI4Kβ1 PI4Kβ2.To...
Growth is the best visible sign of plant comfort. If plants are under stress, a difference in growth with control conditions can indicate that something going wrong (or better). Phytohormones such as auxin, cytokinins, brassinosteroids or giberellins, important regulators and their role was extensively studied. On other hand salicylic acid (SA), phytohormone commonly connected defense responses, under-rated. However, studies SA-overaccumulating mutants directly showed an influence SA on...
Plant flotillins, a subgroup of the SPFH domain protein superfamily, consist three proteins, AtFLOT1, AtFLOT2, and AtFLOT3 in Arabidopsis thaliana. The exact functions flotillins plant cell has not been established yet. In this study we focused on role response to both abiotic biotic stresses phytohormones abscisic acid 1-naphthalene acetic (NAA) A. We observed transcriptomic changes AtFLOT genes high salinity cold, treatment with 22-amino peptide from N-terminal part flagellin (flg22),...
Phospholipase D (PLD) and its product phosphatidic acid are now considered to be one of the key elements numerous physiological processes in plants including salicylic signalling pathway. The presented study investigates transcriptional regulation Brassica napus PLDs following treatments with defense-related stimuli. We cloned eight B. genes encoding members PLDβ, γ, δ isoforms performed phylogenetic analysis ancestor species rapa oleracea, model plant Arabidopsis thaliana. Transcription...
Summary Vesiculation is a process employed by Gram-negative bacteria to release extracellular vesicles (EVs) into the environment. Bacterial EVs contain molecular cargo from donor bacterium and play important roles in bacterial survival growth. Here, we describe EV production plant-pathogenic Pseudomonas syringae pv. tomato DC3000 ( Pto DC3000), causal agent of speck disease. Cultured exhibited structures both on cell surface vicinity cells, observed as outer membrane vesicle (OMV) release....