A5038843313- Lipid metabolism and biosynthesis
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Biofuel production and bioconversion
- Seed Germination and Physiology
- Plant nutrient uptake and metabolism
- Cellular transport and secretion
- Soybean genetics and cultivation
- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Weed Control and Herbicide Applications
- Plant responses to water stress
- Protein Kinase Regulation and GTPase Signaling
- Plant Micronutrient Interactions and Effects
- Bat Biology and Ecology Studies
- Peroxisome Proliferator-Activated Receptors
- Mitochondrial Function and Pathology
- Biotin and Related Studies
- Redox biology and oxidative stress
- Plant Surface Properties and Treatments
- Pesticide and Herbicide Environmental Studies
- Sugarcane Cultivation and Processing
- Magnetic and Electromagnetic Effects
- Plant Gene Expression Analysis
Centre National de la Recherche Scientifique
2006-2024
Sorbonne Université
2010-2024
Institut de Biologie Paris-Seine
2016-2024
Laboratoire de Biologie du Développement
2014-2024
Institut de Pharmacologie et de Biologie Structurale
2019
Laboratoire de physiologie cellulaire et moléculaire
2010-2013
Institut des Sciences des Plantes de Montpellier
2012-2013
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2013
Laboratoire Physiologie Cellulaire & Végétale
2007-2009
Université Joseph Fourier
2007
See also the Commentary by Gupta et al .
Abstract While the presence of a complete shikimate pathway within plant plastids is definitively established, existence cytosolic postchorismate portion still debated. This question alimented by chorismate mutase (CM) cytosol. Until now, only known destiny prephenate, product CM, incorporation into tyrosine (Tyr) and/or phenylalanine (Phe). Therefore, CM suggests that enzymes involved downstream in Tyr or Phe biosynthesis could be present cytosol cells. It was thus particular interest to...
Hydrogen sulfide (H2S) recently emerged as an important gaseous signaling molecule in plants. In this study, we investigated the possible functions of H2S regulating Arabidopsis seed germination. NaSH treatments delayed germination a dose-dependent manner and were ineffective releasing dormancy. Interestingly, endogenous content was enhanced germinating seeds. This increase correlated with higher activity three enzymes (L-cysteine desulfhydrase, D-cysteine desulfhydrase β-cyanoalanine...
Summary Phospholipids have recently been found to be integral elements of hormone signalling pathways. An Arabidopsis thaliana double mutant in two type III phosphatidylinositol‐4‐kinases ( PI 4Ks), pi4k β1β2 , displays a stunted rosette growth. The causal link between 4K activity and growth is unknown. Using microarray analysis, quantitative reverse transcription polymerase chain reaction (RT‐ qPCR ) multiple phytohormone analysis by LC‐MS we investigated the mechanism responsible for...
Phosphatidylinositol-4-phosphate (PtdIns4P) is the most abundant phosphoinositide in plants and precursor of phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P(2)]. This lipid substrate phosphoinositide-dependent phospholipase C (PI-PLC) that produces diacylglycerol (DAG) which can be phosphorylated to phosphatidic acid (PtdOH). In plants, it has been suggested PtdIns4P may also a direct PI-PLC. Whether PtdIns(4,5)P(2) or PI-PLC, its production by phosphatidylinositol-4-kinases (PI4Ks)...
Summary The accurate control of dormancy release and germination is critical for successful plantlet establishment. Investigations in cereals hypothesized a crucial role specific MAP kinase ( MPK ) pathways promoting release, although the identity involved downstream events remain unclear. In this work, we characterized mutants Arabidopsis thaliana 8 8). Mpk8 seeds presented deeper than wild‐type WT) at harvest that was less efficiently alleviated by after‐ripening gibberellic acid...
Low erucic acid rapeseed (LEAR) is characterised by a near absence of very long chain fatty acids in the seed oil which has been correlated with lack acyl‐CoA elongation activity. Here we show that and ATP‐dependent activities microsomes isolated from LEAR embryos associated an β‐ketoacyl‐CoA synthase activity encoded Bn‐ 1 ( FAE1 ) genes. Size exclusion chromatography solubilised revealed presence high molecular mass elongase complex was absent seeds. Although transcripts for genes were...
Phosphoinositide-dependent phospholipases C (PI-PLCs) are activated in response to various stimuli. They utilize substrates provided by type III-Phosphatidylinositol-4 kinases (PI4KIII) produce inositol triphosphate and diacylglycerol (DAG) that is phosphorylated into phosphatidic acid (PA) DAG-kinases (DGKs). The roles of PI4KIIIs, PI-PLCs, DGKs basal signaling poorly understood. We investigated the control gene expression PI-PLC pathway Arabidopsis thaliana suspension cells. A...
Abstract Protein biotinylation is an original and very specific posttranslational modification, compartmented in plants, between mitochondria, plastids, the cytosol. This reaction modifies activates few carboxylases committed key metabolisms catalyzed by holocarboxylase synthetase (HCS). The molecular bases of this complex compartmentalization relative function each HCS genes, HCS1 HCS2, identified Arabidopsis (Arabidopsis thaliana) are mainly unknown. Here, we showed reverse genetics that...
Seed germination is critical for early plantlet development and tightly controlled by environmental factors. Nevertheless, the signaling networks underlying control remain elusive. In this study, remodeling of Arabidopsis seed phosphoproteome during imbibition was investigated using stable isotope dimethyl labeling nanoLC-MS/MS analysis. Freshly harvested seeds were imbibed under dark or constant light to restrict promote germination, respectively. For each regime, phosphoproteins extracted...
Phosphatidylinositol 4-kinases (PI4Ks) catalyze the first step in synthesis of phosphoinositide pools hydrolysed by phosphoinositide-dependent phospholipase C (PI-PLC) and thus constitute a potential key regulation point this pathway. Twelve putative PI4K isoforms, divided as type-II (AtPI4KIIγ1-8) type-III PI4Ks (AtPI4KIIIα1-2 AtPI4KIIIβ1-2), have been identified Arabidopsis genome. By combination pharmalogical genetic approaches we recently evidenced that AtPI4KIIIβ1 AtPI4KIIIβ2 contribute...
Abstract Despite being one of the few bona fide plant tyrosine phosphatases, RLPH2 has no known substrates. Utilizing phospho-proteomics, we identified activation loop phospho-tyrosine several D-group mitogen activated protein kinases (MPKs) as potential All Arabidopsis D-Group MPKs possess a TDY phosphorylation motif, whereas other (Groups A, B and C) contain TEY motif. Our findings reveal that strong preference for aspartate (D) in TXY providing specificity to exclusively target...
de niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
Enzymic activities and gene expression of oleoylCoA elongase were studied during seed development using two different rapeseed cultivars, high-erucic-acid (HEAR) low-erucic-acid (LEAR). The overall maximal in HEAR between the fourth eighth weeks after pollination (WAP) absent LEAR. 3-ketoacyl-CoA synthase (condensing enzyme, CE) mRNA levels developmental profiles cultivars since detected LEAR at WAP 4 6, respectively. Anti-CE antibodies revealed proteins 60 67 kDa both an additional reacting...