A5060790552- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Microbial Community Ecology and Physiology
- Biocrusts and Microbial Ecology
- Slime Mold and Myxomycetes Research
- Antibiotic Resistance in Bacteria
- Toxin Mechanisms and Immunotoxins
- Toxic Organic Pollutants Impact
- Plant Pathogenic Bacteria Studies
- Synthetic Organic Chemistry Methods
- Enzyme Catalysis and Immobilization
- Antibiotic Use and Resistance
- Marine Sponges and Natural Products
- Algal biology and biofuel production
- Bioactive Natural Diterpenoids Research
- Silk-based biomaterials and applications
- Plant Genetic and Mutation Studies
- Mycotoxins in Agriculture and Food
- Microbial bioremediation and biosurfactants
- Environmental Chemistry and Analysis
- Nanoparticles: synthesis and applications
Czech Academy of Sciences, Institute of Microbiology
2019-2022
University of South Bohemia in České Budějovice
2020-2022
Maharishi Markandeshwar University, Mullana
2022
Czech Academy of Sciences
2019
Papaver somniferum (poppy) is a traditional component of Central and Eastern European cuisine an important oilseed crop in the region. The crucial thread for poppy stable yield pathogen infection. Thus, we need to understand defence mechanisms detail. first robust layer plant immunity, which plays role combat against pathogens, pattern-triggered immunity (PTI). Here, provide insights into PTI poppy. We selected four varieties used food industry. investigated response various peptide...
Wounds are structural and functional disruptions of the skin that occur because an accident. Chronic wounds caused by a breakdown in finely coordinated cascade events occurs during wound healing. Wound healing is long process split into at least three continuous overlapping processes: inflammatory response, proliferative phase leads to tissue repair, third one remodeling. Therefore, studies extensively studied develop techniques can achieve maximum recovery with minimum scar. Several growth...
Heterocytous cyanobacteria are among the most prolific sources of bioactive secondary metabolites, including anabaenopeptins (APTs). A terrestrial filamentous Brasilonema sp. CT11 collected in Costa Rica bamboo forest as a black mat, was studied using multidisciplinary approach: genome mining and HPLC-HRMS/MS coupled with bioinformatic analyses. Herein, we report nearly complete consisting 8.79 Mbp GC content 42.4%. Moreover, on three novel tryptophan-containing APTs; anabaenopeptin 788 (1),...
Microbial cyclic lipopeptides are an important class of antifungal compounds with applications in pharmacology and biotechnology. However, the cytotoxicity many limits their potential as drugs. Here we present a structure-activity relationship study on puwainaphycin/minutissamide (PUW/MIN) family isolated from cyanobacteria. PUWs/MINs variable fatty acid chain lengths differed dynamic cytotoxic effect despite similar IC50 after 48 hours (2.8 μM for MIN A 3.2 PUW F). Furthermore, they...
Heterocytous cyanobacteria are among the most prolific source of bioactive secondary metabolites, including anabaenopeptins (APTs). A terrestrial filamentous Brasilonema sp. CT11 collected in Costa Rica bamboo forest, as black mat was studied using a multidisciplinary approach: genome mining and HPLC-HRMS/MS coupled with bionformatic analyses. Herein, we report nearly complete consisting 8.79 Mbp GC content 42.4%. Moreover, on three novel tryptophane-containing APTs; anabaenopeptin 788 (1),...
Puwainaphycins (PUW) and minutissamides (MIN) are structurally homologous cyclic lipopeptides that exhibit high structural variability possess antifungal cytotoxic activities. While only a minor variation can be found in the amino acid composition of peptide cycle, fatty (FA) moiety varies largely. The effect FA functionalization on bioactivity PUW/MIN chemical variants is poorly understood. A rapid selective liquid chromatography-mass spectrometry-based method led us to identify 13 (1-13)...
Resistance development and exhaustion of the arsenal existing antibacterial agents urgently require an alternative approach toward drug discovery. Herein, we report screening Medicines for Malaria Venture (MMV) Pandemic Response Box (PRB) through a cascade developed to streamline potential compounds with antivirulent properties combat opportunistic pathogen, Pseudomonas aeruginosa. To find agent suppressing production P. aeruginosa virulence factors, assessed in PRB quorum sensing inhibitory...
The regulation of the production oligopeptides is essential in understanding their ecological role complex microbial communities, including harmful cyanobacterial blooms. chemical communication between cyanobacterium and community harbored as epibionts within its phycosphere at an initial stage research, little understood about specificity. Here, we present insight into a bacterial epibiont regulating novel microviridins isolated from Nostoc, ecologically important genus. Microviridins are...
We report the draft genome sequence of Streptomyces sp. strain VITNK9, isolated from a soil sample collected in Vellore district (12.9165°N, 79.1325°E), Tamil Nadu, India, with an assembly size 7,920,076 bp and 72.7% GC content.
Scytophycins, including tolytoxin, represent a class of actin disrupting macrolides with strong antiproliferative effects on human cells. Despite intense research, little attention has been paid to scytophycin-induced cell death or the structural features affecting its potency. We show that tolytoxin and natural analogue, 7-O-methylscytophycin B, lacking hydroxyl substitution in macrolactone ring, differ substantially their cytotoxic effect. Both compounds increase level caspases 3/7, which...
ABSTRACT The regulation of oligopeptides production is essential in understanding their ecological role complex microbial communities including harmful cyanobacterial blooms. chemical communication between the cyanobacterium and community harboured as epibionts within its phycosphere at an initial stage research little understood about specificity. Herein, we present insight into a bacterial epibiont regulating microviridins, well-known elastase inhibitors with presumed anti-grazing effects,...