A5064329300- Protein Kinase Regulation and GTPase Signaling
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Thyroid Cancer Diagnosis and Treatment
- Cell death mechanisms and regulation
- Patient-Provider Communication in Healthcare
- Melanoma and MAPK Pathways
- Respiratory Support and Mechanisms
- Respiratory viral infections research
- Infectious Diseases and Tuberculosis
- Cancer, Hypoxia, and Metabolism
- PI3K/AKT/mTOR signaling in cancer
- Cellular transport and secretion
- Nitric Oxide and Endothelin Effects
- Global Cancer Incidence and Screening
- Facial Trauma and Fracture Management
- Vestibular and auditory disorders
- Parvovirus B19 Infection Studies
- Protein Tyrosine Phosphatases
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Receptor Mechanisms and Signaling
- Peptidase Inhibition and Analysis
- Cytomegalovirus and herpesvirus research
University of Georgia
2021-2024
University of Illinois Chicago
2015-2023
Piedmont Athens Regional
2023
Czech Academy of Sciences, Institute of Microbiology
2022
University of Maryland, Baltimore
2021
Grand River Hospital
2020
Dayanand Medical College & Hospital
2014-2018
National Institute of Technology Durgapur
2010-2017
National Cancer Institute
2015
Frederick National Laboratory for Cancer Research
2015
To determine whether instillation of intratympanic steroids is effective in the treatment sudden idiopathic sensorineural hearing loss.A retrospective chart review all patients who underwent steroid (methylprednisolone and/or dexamethasone) between 1996 and 2002 at a tertiary care university otology clinic. Thirty-three were identified, which 26 met inclusion criteria for having an loss. Pretreatment posttreatment pure-tone audiograms speech discrimination scores compared.Overall, there was...
K-Ras4B, a frequently mutated oncogene in cancer, plays an essential role cell growth, differentiation, and survival. Its C-terminal membrane-associated hypervariable region (HVR) is required for full biological activity. In the active GTP-bound state, HVR interacts with acidic plasma membrane (PM) headgroups, whereas farnesyl anchors membrane; inactive GDP-bound may interact both PM catalytic domain at effector binding region, obstructing signaling nucleotide exchange. Here, using molecular...
Is nucleotide exchange sufficient to activate K-Ras4B? To signal, oncogenic rat sarcoma (Ras) anchors in the membrane and recruits effectors by exposing its effector lobe. With use of NMR molecular dynamics (MD) simulations, we observed that solution, farnesylated guanosine 5'-diphosphate (GDP)-bound K-Ras4B is predominantly autoinhibited hypervariable region (HVR), whereas GTP-bound state favors an activated, HVR-released state. On anionic membrane, catalytic domain adopts multiple...
Ras proteins are small GTPases that act as signal transducers between cell surface receptors and several intracellular signaling cascades. They contain highly homologous catalytic domains flexible C-terminal hypervariable regions (HVRs) differ across isoforms. KRAS is among the most frequently mutated oncogenes in human tumors. Surprisingly, we found HVR of K-Ras4B, thought to minimally impact domain, directly interacts with active site protein. The interaction almost 100-fold tighter...
Background: Active surveillance (AS) of small, low-risk papillary thyroid cancers (PTCs) is increasingly being considered. There limited understanding why individuals with PTC may choose AS over traditional surgical management. Methods: We present a mixed-methods analysis prospective observational real-life decision-making study regarding the choice thyroidectomy or for management localized, PTCs <2 cm in maximum diameter (NCT03271892). Patients were provided standardized medical information...
Background: It is important to understand patient preferences on managing low-risk papillary thyroid cancer (PTC). Methods: We prospectively followed patients with PTC <2 cm in maximal diameter, who were offered the choice of thyroidectomy or active surveillance (AS) at University Health Network (UHN), Toronto, Canada. The primary outcome was frequency AS (percentage confidence interval [CI]). Univariate and multivariable analyses performed identify predictors AS. Results: enrolled 200...
Charged scalars appear in many motivated extensions beyond the Standard Model. We analyze constraints on charged scalar pair production via Drell-Yan process at Large Hadron Collider and interpret them terms of weak isospin quantum numbers. Leveraging experimental limits from existing LHC data phenomenological recast analyses, we place bounds branching ratio scalar, as a function its mass, electric charge, isospin. This approach enables to determine ratios directly data, without appealing...
In calmodulin (CaM)-rich environments, oncogenic
A bacterial toxin effector domain disrupts RAS-ERK signaling by cleaving RAS through an unusual mechanism.
Importance Fear is commonly experienced by individuals newly diagnosed with papillary thyroid cancer (PTC). Objective To explore the association between gender and fears of low-risk PTC disease progression, as well its potential surgical treatment. Design, Setting, Participants This single-center prospective cohort study was conducted at a tertiary care referral hospital in Toronto, Canada, enrolled patients untreated small low risk (&amp;lt;2 cm maximal diameter) that confined to...
Abstract Many pathogenic viruses rely on class I fusion proteins to fuse their viral membrane with the host cell membrane. To drive process, undergo an irreversible conformational change from a metastable prefusion state energetically more stable postfusion state. Mounting evidence underscores that antibodies targeting conformation are most potent, making it compelling vaccine candidate. Here, we establish computational design protocol stabilizes while destabilizing conformation. With this...
In spite of established indications for early operative repair orbital floor fractures 7-10% patients treated nonoperatively develop enophthalmos. Clearly further are required to prevent these post-injury complications. Rounding the inferior rectus muscle on coronal computerized tomography (CT) scan results from a loss soft tissue and bony support may therefore be predictive late enophthalmos.A four-year institutional review was conducted identify with that had been nonoperatively. Patients...
Wounds are structural and functional disruptions of the skin that occur because an accident. Chronic wounds caused by a breakdown in finely coordinated cascade events occurs during wound healing. Wound healing is long process split into at least three continuous overlapping processes: inflammatory response, proliferative phase leads to tissue repair, third one remodeling. Therefore, studies extensively studied develop techniques can achieve maximum recovery with minimum scar. Several growth...
Human metapneumovirus (hMPV) is a leading cause of morbidity and hospitalization among children worldwide, however, no vaccines or therapeutics are currently available for hMPV disease prevention treatment. The fusion (F) protein the sole target neutralizing antibodies. To map immunodominant epitopes on F protein, we isolated panel human monoclonal antibodies (mAbs), mAbs were assessed binding avidity, neutralization potency, epitope specificity. We found majority diverse discovered multiple...
Endothelial-dependent and -independent cGMP-mediated mechanisms of pulmonary vasorelaxation were studied in endotoxin-induced acute lung injury the rat. Concentration-response curves generated (10(-9) to 10(-6) M) for acetylcholine (ACh), A23187, sodium nitroprusside (SNP) 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP) 10(-4) isolated arterial rings preconstricted with phenylephrine 6 h after endotoxin treatment (20 mg/kg ip). Endotoxin produced significantly increased neutrophil...
To determine if rounding of the inferior rectus muscle on coronal computed tomographic (CT) scans predicts disruption periorbita in orbital floor fractures and therefore delayed enophthalmos. Cadaveric study with CT scan analysis. laboratory scanner at a tertiary care hospital London, Ontario. Each orbit each cadaveric head was randomly assigned to have either intact or disrupted periorbita. Progressively larger were made taken before after fracture assess shape muscle. Measurements length...
The human Monoamine oxidase (hMAO) metabolizes several biogenic amine neurotransmitters and is involved in different neurological disorders. Extensive MD simulation studies of dopamine-docked hMAO B structures have revealed the stabilization amino-terminal substrate by a direct water-mediated interaction catalytic tyrosines, Gln206, Leu171 residues. catechol ring stabilized Leu171(C–H)⋯π(Dop)⋯(H–C) Ile199 interaction. Several conserved water molecules are observed to play role recognition...