A5029400285- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Parvovirus B19 Infection Studies
- Pneumonia and Respiratory Infections
- Influenza Virus Research Studies
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Escherichia coli research studies
- Congenital Diaphragmatic Hernia Studies
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Infections and Vaccines
- Immunotherapy and Immune Responses
- Pregnancy-related medical research
- HIV Research and Treatment
- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- COVID-19 epidemiological studies
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Outbreaks Research
- Nicotinic Acetylcholine Receptors Study
- Neonatal Respiratory Health Research
University of Georgia
2012-2025
Scripps Institution of Oceanography
2024
International AIDS Vaccine Initiative
2024
Scripps Research Institute
2024
Soochow University
2023
Piedmont Athens Regional
2023
Jiangsu Province Hospital
2021
Nanjing Medical University
2021
Kansas State University
2017-2020
Jinan University
2016
Single-dose intranasal COVID-19 vaccine prevents infection and inhibits transmission.
Abstract A key barrier to the development of vaccines that induce broadly neutralizing antibodies (bnAbs) against human immunodeficiency virus (HIV) and other viruses high antigenic diversity is design priming immunogens rare bnAb-precursor B cells. The neutralization breadth HIV bnAb 10E8 makes elicitation 10E8-class bnAbs desirable; however, recessed epitope within gp41 envelope trimers poor requires possess a long heavy chain complementarity determining region 3 (HCDR3) with specific...
This study investigated the effect of plant polyphenols on faecal microbiota metabolizing oligosaccharides. The results show that can change pathway degrading FOS or even energy metabolism <italic>in vivo</italic> by altering gut composition.
Broadly neutralizing antibodies (bnAbs) targeting the hemagglutinin (HA) stem of influenza A viruses (IAVs) tend to be effective against either group 1 or 2 viral diversity. In rarer cases, intergroup protective bnAbs can generated by human antibody paratopes that accommodate conserved glycan differences between and stems. We applied germline-engaging nanoparticle immunogens elicit a class cross-group from physiological precursor frequency within humanized mouse model. Cross-group protection...
Osteoarthritis (OA) is the most common chronic joint disease in elderly population. Growing evidence indicates that a balance between autophagy and apoptosis chondrocytes plays key role OA's cartilage degradation. Thus, drugs targeting are potential therapeutic approaches for OA treatment. In previous studies, we found activation of α7 nicotinic acetylcholine receptors (α7-nAChRs) alleviated monosodium iodoacetate (MIA)-induced degradation osteoarthritis pain. To explore functions α7-nAChRs...
ABSTRACT Waning immunity from approved COVID-19 vaccines and emerging variants of SARS-CoV-2 necessitate the need to develop alternative for improved durability broader protection. This work investigates efficacy mucosal, humoral, cellular immunogenicity intranasal, parainfluenza virus 5 (PIV5)-vectored vaccine CVXGA1 in an African green monkey (AGM) nonhuman primate model. A single intranasal dose induced robust sustained humoral immune responses AGMs protected against ancestral WA1 strain...
ABSTRACT Immunization with COVID-19 vaccines has greatly reduced COVID-19-related deaths and hospitalizations from SARS-CoV-2 infection, but waning immunity the emergence of variants capable immune escape indicate need for annual vaccine updates or development different platforms. Parainfluenza virus 5 (PIV5)-vectored intranasal ancestral spike (S) protein (CVXGA1) been shown to be a promising next-generation preclinically is currently being evaluated in humans. This work investigates...
Human metapneumovirus (hMPV) is a leading cause of viral respiratory infection in children, and can severe lower tract infants, the elderly, immunocompromised patients. However, there remain no licensed vaccines or specific treatments for hMPV infection. Although fusion (F) protein sole target neutralizing antibodies, immunological properties F poorly understood. To further define humoral immune response to protein, we isolated two new human monoclonal antibodies (mAbs), MPV458 MPV465. Both...
Human metapneumovirus (hMPV) is a leading cause of viral lower respiratory tract infection in children. The sole target neutralizing antibodies targeting hMPV the fusion (F) protein, class I protein mediating virus-cell membrane fusion. There have been several monoclonal (mAbs) isolated that neutralize hMPV; however, determining antigenic sites on F such antibody generation would assist efforts for effective vaccine design. In this report, isolation and characterization four new human mAbs,...
Abstract Many pathogenic viruses rely on class I fusion proteins to fuse their viral membrane with the host cell membrane. To drive process, undergo an irreversible conformational change from a metastable prefusion state energetically more stable postfusion state. Mounting evidence underscores that antibodies targeting conformation are most potent, making it compelling vaccine candidate. Here, we establish computational design protocol stabilizes while destabilizing conformation. With this...
Human metapneumovirus (hMPV) is a leading cause of morbidity and hospitalization among children worldwide, however, no vaccines or therapeutics are currently available for hMPV disease prevention treatment. The fusion (F) protein the sole target neutralizing antibodies. To map immunodominant epitopes on F protein, we isolated panel human monoclonal antibodies (mAbs), mAbs were assessed binding avidity, neutralization potency, epitope specificity. We found majority diverse discovered multiple...
Enterotoxigenic
Summary The JN.1-sublineage KP.3.1.1 recently emerged as the globally prevalent SARS-CoV-2 variant, demonstrating increased infectivity and antibody escape. We investigated how mutations a deletion in spike protein (S) affect ACE2 binding Mass spectrometry revealed new glycan site at residue N30 altered glycoforms neighboring N61. Cryo-EM structures showed that rearrangement of adjacent residues did not significantly change overall structure, up-down ratio receptor-binding domains (RBDs), or...
Human metapneumovirus (hMPV) is an important cause of viral respiratory disease. In this paper, we report the X-ray crystal structure hMPV fusion (F) protein in postfusion conformation from genotype B.
F4 (K88) and F18 fimbriaed enterotoxigenic Escherichia coli (ETEC) are the predominant causes of porcine postweaning diarrhea (PWD), vaccines considered most effective preventive approach against PWD. Since heterologous DNA integrated into bacterial chromosomes could be effectively expressed with stable inheritance, we chose probiotic EcNc (E. Nissle 1917 prototype cured cryptic plasmids) as a delivery vector to express or both fimbriae sequentially assessed their immune efficacy anti-F4 in...
Infectious diseases caused by pathogenic bacteria are a major threat to human health. Capsular polysaccharides (CPSs) of many have been used as the main components glycoconjugate vaccines against bacterial in clinical practice worldwide, with various degrees success.
Abstract Objective. In clinical proton therapy, the spread-out Bragg peak (SOBP) is commonly used to fit target shape. Dose depositions at microscopic sites vary, even with a consistent absorbed dose ( <?CDATA $D$?> <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" overflow="scroll"> <mml:mi>D</mml:mi> </mml:math> ) in SOBP. present study, monolayer mesh-type cell population models were developed for microdosimetric assessment different SOBP depths. Approach. Normal human bronchial...
Streptococcus pneumoniae remains a leading cause of bacterial pneumonia despite the widespread use vaccines. While vaccines are effective at reducing incidence most serotypes included in vaccines, rise infection due to nonvaccine and moderate efficacy against some vaccine have contributed high disease incidence. Additionally, numerous isolates S. antibiotic or multidrug resistant. Several conserved pneumococcal proteins prevalent majority been examined for their potential as preclinical...
Influenza viruses evolve rapidly, driving seasonal epidemics and posing global pandemic threats. While neuraminidase (NA) has emerged as a vaccine target, shared molecular features of NA antibody responses are still not well understood. Here, we describe cryo-electron microscopy structures the broadly protective human DA03E17, which was previously identified from an H1N1-infected donor, in complex with A/H1N1, A/H3N2, B/Victoria-lineage viruses. DA03E17 targets highly conserved active site...
Abstract Antibodies are crucial therapeutics, comprising a significant portion of approved drugs due to their safety and clinical efficacy. Traditional antibody discovery methods labor-intensive, limiting scalability high-throughput analysis. Here, we improved upon our streamlined approach combining structural analysis bioinformatics infer heavy light chain sequences from electron potential maps serum-derived polyclonal antibodies (pAbs) bound antigens. Using ModelAngelo, an automated...