A5103134916- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Clostridium difficile and Clostridium perfringens research
- Microbial infections and disease research
- Salmonella and Campylobacter epidemiology
- Respiratory viral infections research
- Virology and Viral Diseases
- Vibrio bacteria research studies
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Animal Nutrition and Physiology
- Veterinary medicine and infectious diseases
- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Listeria monocytogenes in Food Safety
- CRISPR and Genetic Engineering
- Physiological and biochemical adaptations
- Meat and Animal Product Quality
- Immunotherapy and Immune Responses
- Animal health and immunology
- Aquaculture disease management and microbiota
- Biochemical effects in animals
- Hepatitis Viruses Studies and Epidemiology
- Vector-Borne Animal Diseases
- Food Allergy and Anaphylaxis Research
University of Illinois Urbana-Champaign
2019-2023
Kansas State University
2017-2021
Jeonbuk National University
2013-2018
There are no vaccines licensed for enterotoxigenic Escherichia coli (ETEC), a leading cause of diarrhea children in developing countries and international travelers. Virulence heterogeneity among strains difficulties identifying safe antigens protective antibodies against STa, potent but poorly immunogenic heat-stable toxin which plays key role ETEC diarrhea, challenges vaccine development. To overcome these challenges, we applied toxoid fusion strategy novel epitope- structure-based...
Campylobacter is a food-borne zoonotic pathogen that causes human gastroenteritis worldwide. bacteria are commensal in the intestines of many food production animals, including ducks and chickens. The objective study was to determine prevalence species domestic ducks, agar dilution method used resistance isolates eight antibiotics. In addition, multilocus sequence typing (MLST) performed types (STs) selected isolates. Between May September 2012, 58 duck farms were analyzed, 56 (96.6%)...
We evaluated the effects of different light-emitting diode (LED) colors between blue and green on growth performance immune response in broilers. A total 1,200 1-day-old Ross broilers were divided randomly into six groups exposed to pure (PB), bright (BB), sky (SB), greenish (GB), (PG), or white (W) using LEDs for 6 weeks. Consequently, body weights higher chickens reared under PB GB day (d) 7 SB d 21 than other groups. Chickens group 42 heaviest among groups, followed by BB significantly...
We conducted surveillance for Riemerella anatipestifer (RA) in wild birds along the East Asian-Australasian flyway South Korea. Detected RA were characterized by serotype, antibiotic susceptibility, and sequence analysis of 16S rRNA gene. collected 944 34 species from 19 Korea's major migratory bird habitats between 2011 2012. identified PCR gene 71/102 (69.6%) pharyngeal swabs 19/944 (2.0%) cloacal birds. Most positives (71/75 [95%] 19/704 [(2.6%] cloacal) three duck (family Anatidae):...
Enterotoxigenic Escherichia coli (ETEC) strains are a leading cause of children’s and travelers’ diarrhea. Developing effective vaccines against this heterologous group has proven difficult due to the varied nature toxins adhesins that determine their pathology. A multivalent candidate vaccine was developed using multi-epitope fusion antigen (MEFA) vaccinology platform shown effectively elicit broad protective antibody responses in mice pigs. However, direct protection ETEC colonization...
An investigation was carried out to determine the prevalence and infection pattern of duck circovirus (DuCV) in subclinical Pekin ducks on South Korean farms. A total 147 samples collected from 92 farms five provinces were examined 2011 2012. The overall DuCV PCR-positive pooled bursa Fabricius liver 21.8% (32/147). increased significantly 3-week-old compared with that 1-week-old (P<0.05). association Riemerella Salmonella infections (10.9%; 16/147) occurred at same level as alone 16/147)....
The production performance, efficacy, and safety of two types vaccines for infectious bursal disease virus (IBDV) were compared with in-ovo vaccination Cobb 500 broiler chickens gross microscopic examination the bursa Fabricius, bursa/body weight (b/B) ratio, flow cytometry, serologic response to Newcastle (NDV) vaccination. One vaccine was a recombinant HVT-IBD vector (HVT as herpesvirus turkeys) other an intermediate plus live IBDV vaccine. A significant difference detected at 21 d. Eight...
Double-mutant heat-labile toxin (dmLT, LTR192G/L211A) of enterotoxigenic Escherichia coli (ETEC) is an effective mucosal adjuvant. Recent studies have shown that dmLT also exhibits adjuvanticity for antigens administered parenterally. In this study, we subcutaneously (SC) immunized mice with the ETEC adhesin-based vaccine, CFA/I/II/IV MEFA (multiepitope fusion antigen), adjuvanted and examined impact on antibody responses specific to seven adhesins in vaccine construction [CFA/I, CFA/II...
F4 (K88) and F18 fimbriaed enterotoxigenic Escherichia coli (ETEC) are the predominant causes of porcine postweaning diarrhea (PWD), vaccines considered most effective preventive approach against PWD. Since heterologous DNA integrated into bacterial chromosomes could be effectively expressed with stable inheritance, we chose probiotic EcNc (E. Nissle 1917 prototype cured cryptic plasmids) as a delivery vector to express or both fimbriae sequentially assessed their immune efficacy anti-F4 in...
Using epitope- and structure-based multiepitope fusion antigen vaccinology platform, we constructed a polyvalent protein immunogen that presents antigenic domains (epitopes) of Vibrio cholerae toxin-coregulated pilus A, cholera toxin (CT), sialidase, hemolysin flagellins (B, C, D), peptides mimicking lipopolysaccharide O-antigen on flagellin B backbone. Mice rabbits immunized intramuscularly with this developed antibodies to all the virulence factors targeted by except lipopolysaccharide....
Riemerella anatipestifer is the causative agent of polyserositis and septicaemia in waterfowl. Twenty-one serotypes have been reported, there a strong variation virulence between strains according to serotype or strain. However, little information available assess virulence, such as virulence-associated genes; thus, it difficult estimate risk from field strains. Hence, we established chicken embryo lethality assay (ELA) model determine R. Three virulent (RA T1, RA T7, V-1) three avirulent...
Enterotoxigenic Escherichia coli (ETEC) producing type Ib heat-stable toxin (STa) are a main cause of children's diarrhea and travelers' diarrhea, thus STa needs to be targeted in ETEC vaccine development. However, because this 19-amino acid is poorly immunogenic, attempts genetically fuse or chemically couple it carrier proteins have been made enhance immunogenicity. In study, we selected one genetic fusion chemical conjugate comparatively evaluate The 3xSTaN12S-mnLTR192G/L211A carrying...
Antibodies that block the adherence of enterotoxigenic Escherichia coli (ETEC) to host intestinal epithelial cells are protective. Multiepitope-fusion-antigens (MEFAs) carrying epitopes ETEC adhesin major subunits or tip minor induced antibodies against adherence. Adherence inhibition effectiveness by subunit versus epitopes, however, has not been comparatively characterized. In this study, we immunized mice with a MEFA MEFA, evaluated anti-adhesin immunogenicity, and examined...
Heat-stable toxin (STa)-producing enterotoxigenic Escherichia coli (ETEC) strains are a top cause of moderate-to-severe diarrhea in children from developing countries and common travelers' diarrhea. Recent progress using STa toxoids toxoid fusions to induce neutralizing anti-STa antibodies has accelerated ETEC vaccine development. However, concern remains regarding whether the derived cross-react with STa-like guanylin uroguanylin, two guanylate cyclase C (GC-C) ligands regulating fluid...
The increasing prevalence and association with moderate-to-severe diarrhea make enterotoxigenic Escherichia coli (ETEC) adhesins CS7, CS12, CS14, CS17, CS21 potential targets of ETEC vaccines. Currently, there are no vaccines licensed to protect against ETEC, a top cause children’s travelers’ diarrhea. Recently, polyvalent adhesin protein (adhesin MEFA-II) was demonstrated induce antibodies that inhibited adherence from these five reduced the enterotoxicity heat-stable toxin (STa), which...
There are no vaccines licensed for enterotoxigenic Escherichia coli (ETEC), a leading bacterial cause of children's diarrhea and travelers' diarrhea. MecVax, multivalent E. vaccine candidate composed two epitope- structure-based polyvalent proteins (toxoid fusion 3xSTaN12S-mnLTR192G/L211A colonization factor antigen [CFA]/I/II/IV multiepitope [MEFA]), is designed to induce broad antiadhesin antitoxin antibodies against heterogeneous ETEC pathovars. When administered intraperitoneally or...
There are no licensed vaccines against enterotoxigenic Escherichia coli (ETEC), a leading cause of children's diarrhea and travelers' diarrhea. Recently, protein-based vaccine candidate MecVax was demonstrated to induce functional antibodies both ETEC toxins (heat-stable toxin [STa] heat-labile [LT]) seven adhesins (CFA/I CS1 CS6) protect clinical or intestinal colonization preclinically. Those studies used intraperitoneal, intramuscular, intradermal routes, dose range for protein antigens,...
Background This research aims to evaluate the feasibility of using avian immunoglobulins (IgY) raised against adhesion factors enterotoxigenic Escherichia coli (ETEC) as prophylaxis diarrheal illness caused by these pathogens. ETEC requires human intestinal epithelial cells a primary step in establishing enteric infection. Therefore, inhibition may prevent such infections and reduce clinical burdens illness. Methods IgY samples were prepared from eggs hens immunized with an adhesin-tip...