A5021789335- Influenza Virus Research Studies
- Trypanosoma species research and implications
- Immune Response and Inflammation
- Research on Leishmaniasis Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Magnolia and Illicium research
- Immune Cell Function and Interaction
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- Clostridium difficile and Clostridium perfringens research
- Parasites and Host Interactions
- 14-3-3 protein interactions
- Oxidative Organic Chemistry Reactions
- Virology and Viral Diseases
- Plant biochemistry and biosynthesis
- Natural product bioactivities and synthesis
- Virus-based gene therapy research
- Gut microbiota and health
- interferon and immune responses
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
South Dakota State University
2013-2023
Brookings Institution
2014
Qatar University
2012
Suez Canal University
2012
University of Veterinary Science
2009
University of Saskatchewan
1997-2008
Maharshi Dayanand Saraswati University
2005
Abstract Understanding the molecular parameters that regulate cross-species transmission and host adaptation of potential pathogens is crucial to control emerging infectious disease. Although microbial pathotype diversity conventionally associated with gene gain or loss, role pathoadaptive nonsynonymous single-nucleotide polymorphisms (nsSNPs) has not been systematically evaluated. Here, our genome-wide analysis core genes within Salmonella enterica serovar Typhimurium genomes reveals a high...
ABSTRACT Antibiotics such as chlortetracycline (CTC) have been used to promote growth of pigs for decades, but concerns over increased antibiotic-resistant infections in humans prompted the development alternative strategies. Developing alternatives antibiotic promoters (AGPs) could be informed by information on mechanisms promotion, notably, how AGPs affect microbial populations gastrointestinal tract. Pigs from three sows were aseptically delivered cesarean section. Six piglets distributed...
Summary Influenza D virus ( IDV ) is a newly described influenza type of the Orthomyxoviridae family that was first isolated from diseased swine in 2011 and has subsequently been detected cattle around world 2014. In addition, serological evidence for infection humans recently established. Despite all progress, full range susceptible hosts this novel yet to be determined, but includes swine, bovine, small ruminants human. This study designed determine if equine possible host emerging virus....
ABSTRACT Influenza D virus (FLUDV) is a novel influenza that infects cattle and swine. The goal of this study was to investigate the replication transmission bovine FLUDV in guinea pigs. Following direct intranasal inoculation animals, detected nasal washes infected animals during first 7 days postinfection. High viral titers were obtained from turbinates lung tissues directly inoculated animals. Further, able transmit pigs sentinel by means contact not aerosol dissemination under...
Given recent evidence suggesting that the heat-labile enterotoxin (LT) provides a colonization advantage for enterotoxigenic Escherichia coli (ETEC) in vivo, we hypothesized LT preconditions host intestinal epithelium ETEC adherence. To test this hypothesis, used an vitro model of adherence to examine role promoting bacterium-host interactions. We present data demonstrating elaboration promotes significant increase E. This phenotype is primarily dependent on inherent ADP-ribosylation...
The influenza virus polymerase complex, consisting of the PA, PB1, and PB2 subunits, is required for transcription replication A viral genome. Previous studies have shown that PB1 serves as a core subunit to incorporate PA into complex by directly interacting with PB2. Despite numerous attempts, largely involving biochemical approaches, specific interaction between subunits has yet be detected. In current study, we developed utilized bimolecular fluorescence complementation (BiFC) study...
The study was aimed at investigating the feasibility of using a poly (amidoamine) (PAMAM) dendrimer as carrier for topical iontophoretic delivery an antisense oligonucleotide (ASO). Bcl-2, anti-apoptotic protein implicated in skin cancer, used model target to demonstrate gene silencing approach. Confocal laser scanning microscopy studies demonstrated that iontophoretically delivered ASO-dendrimer complex can reach viable epidermis porcine skin. In contrast, passively free or complexed ASO...
The study was aimed at systematically investigating the influence of shell composition on particle size, stability, release, cell uptake, permeability, and in vivo gastrointestinal distribution food protein based nanocarriers for oral delivery applications. Three different core-shell were prepared using food-grade biopolymers including zein-casein (ZC) nanoparticles, zein-lactoferrin (ZLF), nanoparticles zein-PEG (ZPEG) micelles. Nile red used as a model hydrophobic dye vitro studies. had...
Eliciting a robust immune response at mucosal sites is critical in preventing the entry of pathogens such as influenza and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This task challenging to achieve without inclusion strong safe adjuvant. Previously, inulin acetate (InAc), plant-based polymer, shown activate toll-like receptor-4 (TLR4) elicit systemic vaccine study investigates potential nanoparticles prepared with InAc (InAc-NPs) an intranasal delivery system generate...
The main objective of this study was to investigate the potential calcium phosphate (CAP) nanoparticles for transcutaneous vaccine delivery. CAP were prepared by nanoprecipitation method followed sequential adsorption sugars and ovalbumin. Nanoparticles characterized using dynamic light scattering, XRD, ATR-FTIR, microscopy methods. In-vitro release ovalbumin from studied in buffer (pH 7.4). In-vivo immunization studies carried out Balb/C mice. size zeta ovalbumin-sugar adsorbed 350 +/- 22.5...
Magnolol, a plant lignan isolated from the bark and seed cones of Magnolia officinalis, has been shown to have chemopreventive effects on chemically-induced skin cancer development. The objectives this investigation are study anticarcinogenic magnolol UVB-induced tumor development in SKH-1 mice, model relevant humans, determine possible role apoptosis cell cycle arrest involved development.UVB-induced carcinogenesis mice was used for determining preventive Western blottings flow cytometric...
The goal of this study was to develop bioadhesive food protein nanoparticles using zein (Z), a hydrophobic corn protein, as the core and whey (WP) shell for oral pediatric drug delivery applications. Lopinavir (LPV), an antiretroviral drug, fenretinide, investigational anticancer agent, were used model drugs in study. particle size ZWP range 200-250 nm, encapsulation efficiency >70%. showed sustained release simulated gastrointestinal fluids. enhanced permeability LPV fenretinide across...
Inflammation is an immune response triggered by pathogens, toxins, and cellular damage, leading to acute or chronic inflammatory conditions affecting multiple organs. The present study evaluates the in-vitro anti-inflammatory potential of aqueous leaf extract Capparis brevis pina DC (ALECB) using protein denaturation membrane stabilization assays. phytochemical screening ALECB confirmed presence alkaloids, flavonoids, tannins, glycosides, saponins, which are known for their pharmacological...
Dietary flaxseed has been shown to prevent azoxymethane (AOM)-induced colorectal cancers in male Fisher rats. The present study was designed investigate the chemopreventive effects of dietary on development intestinal tumors Apc Min mice. mice were divided into five different groups, fed with control (AIN-93M meal), corn meal, oil, and oil supplemented diets. Results showed that significantly decreased ( P < 0.05) tumor multiplicity size small intestine colon as compared control,...
alpha-Santalol, an active component of sandalwood oil, has shown chemopreventive effects on skin cancer in different murine models. However, alpha-santalol cell cycle have not been studied. Thus, the objective this study was to investigate progression both p53 mutated human epidermoid carcinoma A431 cells and wild-type melanoma UACC-62 elucidate mechanism(s) action. MTT assay used determine viability UACC-62; fluorescence-activated sorting (FACS) analysis propidium iodide staining for...
We infected highly susceptible BALB/c and relatively resistant C57BL/6 mice with cloned Trypanosoma congolense followed the effects of these infections on circulating parasite numbers, mouse mortality cytokine expression. controlled their parasitaemia survived for up to 163 ± 12 days, while could not control succumbed infection within 8.4 0.5 days. Susceptible had dramatically higher plasma levels IL‐10 than from day 7 forward. This was preceded by an earlier level induction splenic...
Bovine rotavirus (BRV) and bovine coronavirus (BCV) are important causes of diarrhoea death in newborn calves. Although these viruses belong to distinct viral classes, they both infect intestinal epithelial cells induce similar clinical symptoms. Rotavirus usually an acute infection, but infection can persist reoccur adults. Differences structure outcome prompted us postulate that innate mucosal immune responses would be markedly different following infections. To address this hypothesis,...