A5065163132- Sulfur Compounds in Biology
- Redox biology and oxidative stress
- Glutathione Transferases and Polymorphisms
- Molecular Sensors and Ion Detection
- Asymmetric Synthesis and Catalysis
- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Pharmacogenetics and Drug Metabolism
- Synthesis and Catalytic Reactions
- Synthesis of β-Lactam Compounds
- Synthesis and biological activity
- Eicosanoids and Hypertension Pharmacology
- Nanoplatforms for cancer theranostics
- Chemical Synthesis and Analysis
- Advanced Synthetic Organic Chemistry
- Chemical synthesis and alkaloids
- RNA Interference and Gene Delivery
- Mast cells and histamine
- Pharmaceutical Economics and Policy
- Pharmaceutical Practices and Patient Outcomes
- Cancer Genomics and Diagnostics
- Biosimilars and Bioanalytical Methods
- Synthesis and Characterization of Heterocyclic Compounds
- Phytochemicals and Antioxidant Activities
- Nitric Oxide and Endothelin Effects
South Dakota State University
2014-2024
China Pharmaceutical University
2019-2022
GTx (United States)
2016
The First Hospital of Changsha
2014
PLA 306 Hospital
2014
University of Washington
1994-1998
Duke University
1997
Environmental Protection Agency
1997
McMaster University
1997
Research Triangle Park Foundation
1997
The goal of this study was to determine if depletion glutathione (GSH) and inhibition thioredoxin (Trx) reductase (TrxR) activity could enhance radiation responses in human breast cancer stem cells by a mechanism involving thiol-dependent oxidative stress. following were used inhibit GSH Trx metabolism: buthionine sulfoximine (BSO), synthesis inhibitor; sulfasalazine (SSZ), an inhibitor xc- cysteine/glutamate antiporter; auranofin (Au), or 2-AAPA, GSH-reductase inhibitor. Clonogenic...
Thiol redox state (TRS) is an important parameter to reflect intracellular oxidative stress and associated with various normal abnormal biochemical processes. Agents that can be used increase TRS will valuable tools in TRS-related research. Glutathione reductase (GR) a critical enzyme the homeostasis of TRS. The catalyzes reduction GSSG GSH maintain high GSH:GSSG ratio. Inhibition Despite reports GR inhibitors, N,N-bis(2-chloroethyl)-N-nitrosourea, anticancer drug IC50 = 647 μm against yeast...
Thiol groups play a significant role in various cellular functions. Cellular thiol concentrations can be affected by physiological or pathological factors. A fluorescence imaging agent that effectively and specifically image thiols live cells through microscopy is desirable for cell monitoring. Benzofurazan sulfides 1a–1e were synthesized found to specific fluorogenic agents except 1d. They are not fluorescent but form strong adducts after reacting with sulfide-thiol exchange reaction. On...
Thiol groups in biological molecules play a significant role various physiological functions and pathological conditions. Thiols are divided into two major groups: protein thiols nonprotein thiols. Numerous methods have been reported for thiol assays. Most of these developed glutathione, the principal thiol, despite fact that cellular more abundant than glutathione. Further, usually involve process sample preparation followed by separation method, they time-consuming. We previously series...
Following administration of a single oral dose (400 mg) or RS-ibuprofen (RS-IBP) to humans, novel metabolite was isolated from urine and identified by tandem mass spectrometry as the taurine conjugate IBP (IBP-Tau). The corresponding glycine sought but not detected in these studies. Quantitative analyses indicated that conjugation represents minor biotransformation pathway for (1.52 +/- 0.43% over 24 h, n = 4), it is nonetheless one mechanistic significance requires prior formation coenzyme...
Context: Glutaredoxins (GRX) are involved in the regulation of thiol redox state. GRX-1 is a cytosolic enzyme responsible for catalysis deglutathionylation proteins. To date, very few inhibitors have been reported.Objective: The objective this paper to report 2-acetylamino-3-[4-(2-acetylamino-2-carboxyethyl-sulfanylthiocarbonylamino)phenylthiocarbamoylsulfanyl]propionic acid (2-AAPA) as an inhibitor human GRX-1.Materials and methods: mechanism inhibition was investigated using dialysis,...
Glutathione disulfide (GSSG) is an endogenous peptide and the oxidized form of glutathione. The impacts GSSG on cell function/dysfunction remain largely unexplored due to a lack method specifically increase intracellular GSSG. We recently developed liposomes that can affected 3 4 essential steps (cell detachment, migration, invasion, adhesion) cancer metastasis in vitro and, accordingly, produced significant inhibition vivo. In this investigation, effect growth was investigated with B16-F10...
Abstract Background Microtubules have been one of the most effective targets for development anticancer agents. Cancer cells treated by these agents are characterized cell arrest at G 2 /M phase. Microtubule-targeting drugs are, therefore, referred to as antimitotic However, clinical application current is hampered emerging drug resistance which major cause cancer treatment failure. The success and has prompted a search new agents, especially those with novel mechanisms action. aim this...
Celecoxib has been found to be effective in cancer prevention and treatment. Its combination with other chemotherapeutic agents was reported produce synergistic/additive effects on various cancers. Dacarbazine (DTIC) is one of the most commonly used drugs treatment metastatic melanoma. This investigation aimed determine in-vitro in-vivo drug celecoxib DTIC melanoma growth metastasis. Melanoma cells B16-F10 SK-MEL-28, female C57BL/6 mice were for study. Our data showed that significant...
Previous studies from this laboratory have indicated that alpha-santalol (5%) provides chemopreventive effects in 7,12-dimethylbenz[a]anthracene (DMBA)-initiated and 12-O-tetradecanoylphorbol-13-acetate (TPA)-promoted skin cancer CD-1 SENCAR mice. Skin development is associated with increased ornithine decarboxylase (ODC) activity, DNA synthesis rapid proliferation of epidermal cells. The purpose investigation was to determine the various concentrations (1.25% 2.5%) on DMBA-initiated...
Cancer metastasis is the major cause of cancer mortality. Despite extensive research efforts, effective treatment for still lacking. involves 4 essential steps: cell detachment, migration, invasion, and adhesion. Detachment first required step metastasis. Glutathione disulfide (GSSG) derived from oxidation glutathione (GSH), which present in biological systems millimolar concentration. Although GSSG commercially available, impact on functions/dysfunctions has not been fully explored due to...