A5000583754- Potato Plant Research
- Plant Pathogens and Resistance
- Berry genetics and cultivation research
- Plant Stress Responses and Tolerance
- Plant Physiology and Cultivation Studies
- Phytochemicals and Antioxidant Activities
- Postharvest Quality and Shelf Life Management
- Photosynthetic Processes and Mechanisms
- Insect and Pesticide Research
- Plant Gene Expression Analysis
- Bee Products Chemical Analysis
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Meat and Animal Product Quality
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Healthcare and Venom Research
- Pomegranate: compositions and health benefits
- Phytochemical Studies and Bioactivities
- Marine and coastal plant biology
- Plant Surface Properties and Treatments
- Plant Parasitism and Resistance
- Genomics, phytochemicals, and oxidative stress
- Mycotoxins in Agriculture and Food
James Hutton Institute
2014-2024
University of Leeds
2024
Bridge University
2022
Abertay University
2022
Biomathematics and Statistics Scotland
2008
University of Dundee
2007
Czech Agrifood Research Center
1988
Clostridium difficile virulence is driven primarily by the processes of toxinogenesis and sporulation, however many in vitro experimental systems for studying C. physiology have arguably limited relevance to human colonic environment. We therefore created a more physiologically-relevant model milieu study gut pathogen biology, incorporating faecal water (FW) into growth media assessing physiological effects this on strain 630. identified novel set difficile-derived metabolites culture...
Although significant work has been undertaken regarding the response of model and crop plants to heat shock during acclimatory phase, few studies have examined steady-state mild stress encountered in temperate agriculture. In present work, we therefore exposed tuberizing potato mildly elevated temperatures (30/20 °C, day/night) for up 5 weeks compared tuber yield, physiological biochemical responses, leaf metabolomes transcriptomes with grown under optimal conditions (22/16 °C). Growth at...
Potato production is often constrained by abiotic stresses such as drought and high temperatures which are present in combination. In the work, we aimed to identify key mechanisms processes underlying single combined stress tolerance comparative analysis of tolerant susceptible cultivars. Physiological data indicated that cultivars Desiree Unica were while Agria Russett Burbank susceptible. Abiotic caused a greater reduction photosynthetic carbon assimilation was associated with lower leaf...
Aims: Aphids, like other insects, are probably unable to synthesize vitamin C (ascorbic acid), which is therefore an essential dietary nutrient that has be obtained from the host plant. Plant responses aphids involve hormones such as salicylic acid (SA), jasmonic (JA), and abscisic (ABA), but hormone/redox interactions remain poorly characterized. We investigated signaling in response of Arabidopsis thaliana infestation by aphid Myzus persicae, focusing on between ascorbic ABA, together with...
Agricultural nitrous oxide (N2O) pollution resulting from the use of synthetic fertilizers represents a significant contribution to anthropogenic greenhouse gas emissions, providing rationale for reduced nitrogen (N) fertilizers. Nitrogen limitation results in extensive systems rebalancing that remodels metabolism and defence processes. To analyse regulation underpinning these responses, barley (Horedeum vulgare) seedlings were grown 7 d under N-deficient conditions until net photosynthesis...
Anthocyanin content of potato tubers is a trait that attracting increasing attention as the potential nutritional benefits this class compound become apparent. However, our understanding tuber anthocyanin accumulation not complete. The aim study was to use microarray investigate gene expression patterns associated with purple anthocyanins. advanced selections, CO97216-3P/PW and CO97227-2P/PW, developed by conventional breeding procedures, produced incomplete flesh pigmentation. This feature...
Phytochemical diversity with respect to a range of polar (including amino acids, organic sugars, and sugar alcohols) nonpolar fatty alkanols, sterols) metabolites was examined within tubers from total 29 genetically diverse potato cultivars Chilean landraces using metabolomics approach by gas chromatography−mass spectrometry. From principal component analysis the metabolite data there insufficient variation differentiate majority landraces. Analysis all profiles revealed separation two...
Abstract Plant polyphenolics continue to be the focus of attention with regard their putative impact on human health. An increasing and ageing population means that nutrition nutritional enhancement or optimisation our foodstuffs is paramount. Using raspberry as a model, we have shown how modern metabolic profiling approaches can used identify changes in level beneficial fruit breeding segregating populations these components determined by genetic and/or environmental control. Interestingly,...
Abstract The redox state of the apoplast is largely determined by ascorbate oxidase (AO) activity. influence AO activity on leaf acclimation to changing irradiance was explored in wild‐type (WT) and transgenic tobacco ( Nicotiana tobaccum ) lines containing either high [pumpkin (PAO)] or low [tobacco (TAO)] at [low light (LL); 250 μ mol m −2 s −1 ] [high (HL); 1600 following transition from HL LL. activities changed over photoperiod, particularly PAO plants. had little effect ascorbate,...
The (poly)phenols in ileal fluid after ingestion of raspberries were analyzed by targeted and nontargeted LC-MS(n) approaches. Targeted approaches identified major anthocyanin ellagitannin components at varying recoveries with considerable interindividual variation. Nontargeted analysis using an orbitrap mass spectrometer gave exact MS data which sifted a software program to select peaks that changed significantly supplementation. This method confirmed the recovery but also novel...
Studies on broccoli (Brassica oleracea var. italica) indicate beneficial effects against a range of chronic diseases, commonly attributed to their bioactive phytochemicals. Sulforaphane, the form glucoraphanin, is formed by action indigenous enzyme myrosinase. This study explored role that digestion and cooking practices play in bioactivity bioavailability, especially rarely considered dose delivered colon.The antimicrobial activity sulforaphane extracts from raw, cooked plus mustard seeds...
Phytochemical diversity was examined by gas chromatography−mass spectrometry in tubers of genotypes belonging to groups Andigena, Phureja, Stenotomum, and Tuberosum the potato, Solanum tuberosum. Polar extracts (mainly amino acids, organic sugars, sugar alcohols) nonpolar fatty alcohols, sterols) were examined. There a large range levels metabolites, including those such as asparagine, fructose, glucose, that are important tuber quality, offering considerable scope for selecting germplasm...
Abstract Recent advances have defined some of the components photoperiodic signalling that lead to tuberization in potato including orthologues FLOWERING LOCUS T ( StSP6A ) and CYCLING DOF FACTOR St CDF1 ). The aim current study is investigate molecular basis permissive tuber initiation under long days S olanum tuberosum N eo‐ uberosum by comparative analysis with an obligate short‐day . ssp. A ndigena accession. We show accession, but not ndigena, contains alleles encode StCDF1 proteins...
Abstract This paper describes an experiment to identify volatile organic compounds (VOCs) from a range of three bacteria and one yeast strain that had previously been shown be inhibitory selected sapstain fungi. The were cultured on two media, malt extract (ME) tryptone soya (TS) the VOCs trapped chromatographic adsorbant before being analysed by Integrated Thermal Desorption—GC-MS. Since fungi only inhibited produced TS it was possible use Principle Component Analysis highlight individual...
WHIRLY1 is largely targeted to plastids, where it a major constituent of the nucleoids. To explore functions in barley (Hordeum vulgare), RNA interference-knockdown lines (W1-1, W1-7, and W1-9) that have very low levels HvWHIRLY1 transcripts were characterized plants grown under optimal stress conditions. The WHIRLY1-1 (W1-1), W1-9 phenotypically similar wild type but produced fewer tillers seeds. Photosynthesis rates all lines, W1-1, leaves had significantly more chlorophyll less sucrose...
Strawberry cultivars grown at different locations in Europe showed genotype- and environment-dependent variation total phenol anthocyanin contents. This study focused on the compositional diversity of tannins from these using a high-throughput liquid chromatography-mass spectrometric (LC-MS) technique. Cultivars Germany differences relative amounts proanthocyanidins (PACs) ellagitannins (ETs). three European their PAC/ET balance. 'Elsanta' Switzerland had higher levels ET-derived than...
The mechanisms underpinning plant perception of phloem-feeding insects, particularly aphids, remain poorly characterized. Therefore, the role apoplastic redox state in controlling aphid infestation was explored using transgenic tobacco (Nicotiana tabacum) plants that have either high (PAO) or low (TAO) ascorbate oxidase (AO) activities relative to wild type. Only a small number leaf transcripts and metabolites were changed response genotype, cell wall composition largely unaffected. Aphid...