A5007646303- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Plant Disease Resistance and Genetics
- CRISPR and Genetic Engineering
- Plant and Fungal Interactions Research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Animal Virus Infections Studies
- Insect-Plant Interactions and Control
- Cocoa and Sweet Potato Agronomy
- Plant Parasitism and Resistance
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Resistance
- Bacteriophages and microbial interactions
- Plant Reproductive Biology
- Pineapple and bromelain studies
- Viral Infections and Vectors
- Fungal Plant Pathogen Control
- Animal Disease Management and Epidemiology
- Photosynthetic Processes and Mechanisms
- Plant Disease Management Techniques
Biologie du Fruit et Pathologie
2015-2024
Université de Bordeaux
2008-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2001-2024
Unité de Recherche Œnologie
1990-2020
Institut National de la Recherche Agronomique
1996-2015
Sainsbury Laboratory
2012
University of Liège
2012
Centre National de la Recherche Scientifique
1995-2012
John Innes Centre
2012
Centre de Biophysique Moléculaire
2012
Abstract Remorins (REMs) are proteins of unknown function specific to vascular plants. We have used imaging and biochemical approaches in situ labeling demonstrate that REM clusters at plasmodesmata ∼70-nm membrane domains, similar lipid rafts, the cytosolic leaflet plasma membrane. From a manipulation levels transgenic tomato (Solanum lycopersicum) plants, we show Potato virus X (PVX) movement is inversely related accumulation. can interact physically with protein TRIPLE GENE BLOCK PROTEIN1...
Abstract The eIF4E and eIF(iso)4E cDNAs from several genotypes of lettuce (Lactuca sativa) that are susceptible, tolerant, or resistant to infection by Lettuce mosaic virus (LMV; genus Potyvirus) were cloned sequenced. Although Ls-eIF(iso)4E was monomorphic in sequence, three types Ls-eIF4E differed point sequence variations, a short in-frame deletion one them. amino acid variations specific 1 2 predicted be located near the cap recognition pocket homology-based tridimensional protein model....
Five transcription units of the Pseudomonas solanacearum hrp gene cluster are required for secretion HR-inducing PopA1 protein. The nucleotide sequences two these, 1 and 3, have been reported. Here, we present sequence three other units, 2, 4 7, which together predicted to code 15 genes. This brings total number Hrp proteins encoded by these five 20, including HrpB, positive regulatory protein, HpaP, is apparently not plant interactions. Among 18 proteins, eight belong protein families...
Plants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant membrane is involved in responses viruses mostly unknown. Here, we show that cells sense Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins subsequently trigger activation a calcium-dependent kinase. We Arabidopsis thaliana CALCIUM-DEPENDENT KINASE 3-interacts with group REMORINs vivo, phosphorylates intrinsically disordered N-terminal domain Group...
The helper component proteinase (HC-Pro) is a key protein encoded by plant viruses of the genus Potyvirus. HC-Pro involved in different steps viral cycle, aphid transmission, replication, and virus cell-to-cell systemic movement suppressor post-transcriptional gene silencing. Structural knowledge required to better understand its multiple functions. To this aim, we purified His-tagged wild-type N-terminal deletion mutant (DeltaHC-Pro) from plants infected with recombinant potyviruses....
The translation initiation factors eIF4E and eIF(iso)4E play a key role during virus infection in plants. During mRNA translation, provides the cap‐binding function is associated with protein eIF4G to form eIF4F complex. Susceptibility analyses of Arabidopsis mutants knocked‐out for At ‐ genes showed that are indispensable potyvirus infection. colonization pattern by viral recombinant carrying GFP indicated involved at very early step. Like eIF4E, isoforms selectively recruited Moreover,...
The formation of plasma membrane (PM) microdomains plays a crucial role in the regulation signaling and trafficking. Remorins are plant-specific family proteins organized six phylogenetic groups, group 1 among few plant known to specifically associate with rafts. As such, they valuable understand molecular bases for PM lateral organization plants. However, little is about structural determinants underlying specific association We used structure-function approach identify short C-terminal...
Summary To infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology supply the lack of resistance alleles, new strategies need to developed increase spectra durability without impairing plant development. Here, we assess how targeted allele modification Arabidopsis thaliana translation initiation eIF 4E1 lead broad...
The virus protein linked to the genome (VPg) of plant potyviruses is a 25‐kDa covalently attached genomic RNA 5′ end. It was previously reported that VPg binds specifically eIF4E, mRNAcap‐binding eukaryotic translation initiation complex. We performed spectroscopic study interactions between lettuce eIF4E and from mosaic (LMV). cap analogue m 7 GDP bind at two distinct sites with similar affinity ( K d = 0.3 µ ). A deeper examination interaction pathway showed binding one ligand induces...
Previous resistance analyses of Arabidopsis thaliana mutants knocked out for eukaryotic translation initiation factors showed that disruption the At-eIF(iso)4E or both At-eIF(iso)4G1 and At-eIF(iso)4G2 genes resulted in against turnip mosaic virus (TuMV). This study selected TuMV virulent variants overcame this two independent mutations region coding viral genome-linked protein (VPg) were sufficient to restore virulence At-eIF(iso)4G1×At-eIF(iso)4G2 knockout plants. As a VPg–eIF(iso)4E...
The Triple Gene Block 1 (TGBp1) protein encoded by the Potato virus X is a multifunctional that acts as suppressor of RNA silencing or facilitates passage from cell to promoting plasmodesmata opening. We previously showed membrane raft StRemorin1.3 able impair PVX infection. Here, we show overexpressed does not activity TGBp1, but affects its ability increase permeability. A similar effect on permeability was observed with other movement proteins, suggesting REM general regulator...
SUMMARY The capacity of Lettuce mosaic virus to overcome the lettuce resistance conferred by mo1 1 and 2 alleles gene for eukaryotic translation initiation factor 4E (eIF4E) was analysed using reverse genetics. Mutations in genome‐linked protein (VPg) allowed only be overcome, but mutations C‐terminal portion cylindrical inclusion (CI) both overcome. Site‐directed mutagenesis pinpointed a key role amino acid at position 621 virulence. This is first example involvement potyviral CI breaking...
The eukaryotic translation initiation factor 4E (eIF4E) (the cap-binding protein) is involved in natural resistance against several potyviruses plants. In lettuce, the recessive genes mo1(1) and mo1(2) Lettuce mosaic virus (LMV) are alleles coding for forms of eIF4E unable, or less effective, to support accumulation. A recombinant LMV expressing a susceptible lettuce variety from its genome was able produce symptoms varieties. order identify amino acid residues necessary viral infection, we...
Approaches to simplify and accelerate the construction of full-length infectious cDNA clones for plant potyviruses have been described, based on cloning strategies involving in vitro ligation or homologous recombination yeast. In present study, we developed a faster more efficient system using Gibson assembly (GA), engineer Lettuce mosaic virus (LMV) clone expressing an ectopic mcherry-tagged VPg (Viral protein genome-linked) planta subcellular localization viral infection context. Three...
The RNA genome of a resistance-breaking isolate Lettuce mosaic virus (LMV-E) was engineered to express the jellyfish green fluorescent protein (GFP) or β-glucuronidase (GUS) fused helper-component proteinase (HC-Pro) study LMV invasion and spread in susceptible resistant lettuce cultivars. Virus accumulation movement were monitored by either histochemical GUS assays detection GFP fluorescence under UV light. GFP- GUS-tagged viruses systemically cultivars Trocadero Vanguard, where they...
In plants, the ubiquitin/26S proteasome system (UPS) plays a central role in protein degradation and is involved many steps of defence mechanisms, regardless types pathogen targeted. addition to its proteolytic activities, UPS ribonuclease (RNase) activity, previously detected 20S preparations from cauliflower sunflower (Helianthus annuus), has been shown specifically target plant viral RNAs vitro. this study, we show that recombinant Arabidopsis thaliana proteasomal α(5) subunit expressed...
REMORIN proteins belong to a plant-specific multigene family that localise in plasma membrane nanodomains and plasmodesmata. We previously showed Nicotiana benthamiana, group 1 StREM1.3 limits the cell-to-cell spread of potexvirus without affecting viral replication. This prompted us check whether an effect on propagation could apply potyvirus species Turnip mosaic virus (TuMV) Potato A (PVA). Our results show transient or stable overexpression transgenic lines increases propagation, while...