A5002928792- Legume Nitrogen Fixing Symbiosis
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Computational Drug Discovery Methods
- Alzheimer's disease research and treatments
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Protein Structure and Dynamics
- Crystallography and molecular interactions
- Cellular transport and secretion
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Eicosanoids and Hypertension Pharmacology
- Machine Learning in Bioinformatics
- Cutaneous lymphoproliferative disorders research
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- CNS Lymphoma Diagnosis and Treatment
- Tryptophan and brain disorders
- Organic and Molecular Conductors Research
- Metalloenzymes and iron-sulfur proteins
- Fatty Acid Research and Health
- Erythrocyte Function and Pathophysiology
- Supramolecular Self-Assembly in Materials
Masaryk University
2022-2024
University Hospital Brno
2022-2024
St. Anne's University Hospital Brno
2022-2024
Institut Polytechnique de Bordeaux
2018-2024
Centre National de la Recherche Scientifique
2005-2023
Institut Européen de Chimie et Biologie
2018-2023
Chimie et Biologie des Membranes et des Nanoobjects
2018-2023
Laboratoire de Biogenèse Membranaire
2018-2022
Université de Bordeaux
2018-2022
Institut de génétique et de biologie moléculaire et cellulaire
2005
Plants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant membrane is involved in responses viruses mostly unknown. Here, we show that cells sense Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins subsequently trigger activation a calcium-dependent kinase. We Arabidopsis thaliana CALCIUM-DEPENDENT KINASE 3-interacts with group REMORINs vivo, phosphorylates intrinsically disordered N-terminal domain Group...
Abstract Background Apolipoprotein E (ApoE) ε4 genotype is the most prevalent risk factor for late-onset Alzheimer’s Disease (AD). Although ApoE4 differs from its non-pathological ApoE3 isoform only by C112R mutation, molecular mechanism of proteinopathy unknown. Methods Here, we reveal aggregation using a combination experimental and computational techniques, including X-ray crystallography, site-directed mutagenesis, hydrogen-deuterium mass spectrometry (HDX-MS), static light scattering...
Computational study of the effect drug candidates on intrinsically disordered biomolecules is challenging due to their vast and complex conformational space. Here, we developed a comparative Markov state analysis (CoVAMPnet) framework quantify changes in distribution dynamics biomolecule presence absence small organic candidate molecules. First, molecular trajectories are generated using enhanced sampling, molecule candidates, ensembles soft models (MSMs) learned for each system unsupervised...
Nanodomains are dynamic membrane subcompartments, enriched in specific lipid and protein components that act as functional platforms to manage an abundance of cellular processes. The remorin plants is a well-established nanodomain marker widely serves paradigm study clustering. Located at the inner leaflet plasma membrane, remorins perform essential functions during signalling. Using deuterium phosphorus solid-state NMR, we inquire on molecular determinants lipid-protein protein-protein...
ABSTRACT Computational study of the effect drug candidates on intrinsically disordered biomolecules is challenging due to their vast and complex conformational space. Here we developed a Comparative Markov State Analysis (CoVAMPnet) framework quantify changes in distribution dynamics biomolecule presence absence small organic candidate molecules. First, molecular trajectories are generated using enhanced sampling, molecule candidates, ensembles soft state models (MSMs) learned for each...
<title>Abstract</title> Remorins are multifunctional proteins, regulating immunity, development and symbiosis in plants. When associating to the membrane, remorins sequester specific lipids into functional membrane nanodomains. The multigenic protein family contains six groups, classified upon their protein-domain composition. Membrane targeting of occurs independently from secretory pathway. Instead, they directed different nanodomains depending on phylogenetic group. All members contain a...
Abstract Remorins are a family of multigenic phosphoproteins the plasma membrane, involved in biotic and abiotic plant interaction mechanisms, partnering molecular signaling cascades. Signaling activity remorins depends on their phosphorylation states subsequent clustering into nano-sized membrane domains. The presence coiled-coil domain C-terminal is crucial to anchor negatively charged domains, however exact role N-terminal intrinsically disordered (IDD) protein lipid interactions largely...
ABSTRACT The dynamic regulation of the plasma membrane (PM) organization at nanoscale emerged as a key element shaping outcome host-microbe interactions. Protein into nanodomains (ND) is often assumed to be linked activation cellular processes. In contrast, we have previously shown that phosphorylation Solanum tuberosum REM1.3 (StREM1.3) N-terminal domain disperses its native ND and promotes inhibitory effect on Potato Virus X (PVX) cell-to-cell movement. Here, show StREM1.3 modify chemical...
Abstract Background Apolipoprotein E (ApoE) ε4 genotype is the most prevalent risk factor for late-onset Alzheimer’s Disease (AD). Although ApoE4 differs from its non-pathological ApoE3 isoform only by C112R mutation, molecular mechanism of proteinopathy unknown. Methods Here, we reveal aggregation using a combination experimental and computational techniques, including X-ray crystallography, site-directed mutagenesis, hydrogen-deuterium mass spectrometry (HDX-MS), static light scattering...