A5027059523- Glycosylation and Glycoproteins Research
- Esophageal Cancer Research and Treatment
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- Heat shock proteins research
- Galectins and Cancer Biology
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- thermodynamics and calorimetric analyses
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Pancreatic and Hepatic Oncology Research
- Alzheimer's disease research and treatments
- Tryptophan and brain disorders
- Colorectal Cancer Treatments and Studies
- Ubiquitin and proteasome pathways
- Drug Transport and Resistance Mechanisms
- Immune Response and Inflammation
- Ferroptosis and cancer prognosis
- Research on Leishmaniasis Studies
- Fungal Infections and Studies
- interferon and immune responses
- DNA Repair Mechanisms
- Neutropenia and Cancer Infections
- Cytokine Signaling Pathways and Interactions
Masaryk Memorial Cancer Institute
2019-2024
University Hospital Ostrava
2023
Národný Onkologický Ústav
2019
Abstract Breast cancer is a highly heterogeneous disease. Its intrinsic subtype classification for diagnosis and choice of therapy traditionally relies on the presence characteristic receptors. Unfortunately, this often not sufficient precise prediction disease prognosis treatment efficacy. The N-glycan profiles 145 tumors 10 healthy breast tissues were determined using Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry. tumor samples classified into Mucinous,...
Abstract Background Apolipoprotein E (ApoE) ε4 genotype is the most prevalent risk factor for late-onset Alzheimer’s Disease (AD). Although ApoE4 differs from its non-pathological ApoE3 isoform only by C112R mutation, molecular mechanism of proteinopathy unknown. Methods Here, we reveal aggregation using a combination experimental and computational techniques, including X-ray crystallography, site-directed mutagenesis, hydrogen-deuterium mass spectrometry (HDX-MS), static light scattering...
BackgroundEfforts to address the poor prognosis associated with esophageal adenocarcinoma (EAC) have been hampered by a lack of biomarkers identify early disease and therapeutic targets. Despite extensive efforts understand somatic mutations EAC over past decade, gap remains in understanding how atlas genomic aberrations this cancer impacts proteome which variants are importance for phenotype.MethodsWe performed quantitative proteomic analysis 23 EACs matched adjacent normal gastric tissues....
Abstract Congenital dyserythropoietic anemia type I (CDA-I) is a rare hereditary disease characterized by ineffective erythropoiesis, spongy heterochromatin of erythroblasts, and associated mutations in two proteins – Codanin1 CDIN1. regulates nucleosome assembly through histone chaperone ASF1. The function recently discovered CDIN1 remains unknown, but has been known to interact directly with the C-terminus Codanin1. Despite critical role identified CDIN1, effects CDA-I-related at molecular...
Abstract Background Addressing the poor prognosis associated with esophageal adenocarcinoma (EAC) has been hindered by absence of biomarkers for early disease detection and therapeutic targets. Despite extensive research on somatic mutations EAC, impact genomic aberrations protein expression cancer phenotype remains unclear. Methods We conducted a quantitative proteomic analysis using tumour tissue patient-matched adjacent normal gastric tissues from 23 patients undergoing resection EAC....
A comparative canine-human therapeutics model is being developed in B-cell lymphoma through the generation of a hybridoma cell that produces murine monoclonal antibody specific for canine CD20. The two light chains, chain-3, and chain-7. However, contribution either chain to authentic full-length derived IgG undefined. Mass spectrometry was used identify only one chain-7, as predominating IgG. Gene synthesis created recombinant murine-canine chimeric expressing chain-7 reconstituted binding...
Interferon induced transmembrane proteins (IFITMs) play a dual role in the restriction of RNA viruses and cancer progression, yet mechanism their action remains unknown. Currently, there is no data about basic biochemical features or biophysical properties IFITM1 protein. In this work, we report on description characterization three conformational variants/oligomeric species recombinant protein derived from an
Stress responses play a vital role in cellular survival against environmental challenges, often exploited by cancer cells to proliferate, counteract genomic instability, and resist therapeutic stress. Heat shock factor protein 1 (HSF1), central transcription stress response pathways, exhibits markedly elevated activity cancer. Despite extensive research into the transcriptional of HSF1, mechanisms underlying its activation remain elusive. Upon exposure conditions that induce damage,...
Readthrough of a translation termination codon is regulated by ribosomal A site recognition and insertion near-cognate tRNAs. Small molecules exist that mediate incorporation amino acids at the stop production full-length, often functional protein but defining actual acid incorporated remains challenging area. Herein, we report on development human cell model can be used to determine whether rules developed using mass spectrometry define type placed premature (PTC) during readthrough...
Allosteric changes imposed by post-translational modifications regulate and differentiate the functions of proteins with intrinsic disorder regions. HDM2 is a hub protein large interactome different cellular functions. It best known for its regulation p53 tumour suppressor. Under normal conditions, ubiquitinates degrades 26S proteasome but after DNA damage, switches from negative to positive regulator binding mRNA promote translation mRNA. This change in activity governed ataxia...
Abstract Stress responses play a pivotal role in normal physiology, feature exploited by cancer cells to facilitate growth, counteract genomic instability, and resist therapeutic stress. Heat shock factor-1 (HSF1), major stress-response transcription factor, exhibits significant upregulation activity cancer. While HSF1's is relatively well-known, the mechanisms governing its activation, particularly tumors, remain unclear. To determine whether HSF1 primary sensor of proteotoxic stress, we...
HDMX and its homologue HDM2 are two essential proteins for the cell; after genotoxic stress, both phosphorylated near to their RING domain, specifically at serine 403 395, respectively. Once phosphorylated, can bind p53 mRNA enhance translation; however, recognize protein provoke degradation under normal conditions. has been well-recognized as an E3 ubiquitin ligase, whereas it reported that even with high similarity between domains of homologs, does not have ligase activity. Despite this,...
Východiska: Glykosylace je posttranslační modifikace, která zapojena do mnoha bio logických procesů a významně zasahuje i dějů spojených s nádorovou progresí.Změny glykanových struktur na povrchu nádorových buněk způsobené alterujícími hladinami exprese glykosyltransferáz glykosidáz ovlivňují proliferaci, adhezi, migraci buněčnou signalizaci
Abstract Background Apolipoprotein E (ApoE) ε4 genotype is the most prevalent risk factor for late-onset Alzheimer’s Disease (AD). Although ApoE4 differs from its non-pathological ApoE3 isoform only by C112R mutation, molecular mechanism of proteinopathy unknown. Methods Here, we reveal aggregation using a combination experimental and computational techniques, including X-ray crystallography, site-directed mutagenesis, hydrogen-deuterium mass spectrometry (HDX-MS), static light scattering...
Abstract Background Efforts to address the poor prognosis associated with esophageal adenocarcinoma (EAC) have been hampered by a lack of biomarkers identify early disease and therapeutic targets. Despite extensive efforts understand somatic mutations EAC over past decade, gap remains in understanding how atlas genomic aberrations this cancer impacts proteome. Differences transcript corresponding protein abundances remain under-explored, leaving gaps our mechanisms underlying disease....