A5073160270- Sarcoma Diagnosis and Treatment
- Cardiac tumors and thrombi
- Cancer Immunotherapy and Biomarkers
- Vascular Tumors and Angiosarcomas
- Angiogenesis and VEGF in Cancer
- Immune Cell Function and Interaction
- Immune cells in cancer
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Ideological and Political Education
- Gut microbiota and health
- Advanced Materials Characterization Techniques
- Cancer, Stress, Anesthesia, and Immune Response
- Epigenetics and DNA Methylation
- Gastrointestinal motility and disorders
- Clostridium difficile and Clostridium perfringens research
- Fungal Infections and Studies
- Extracellular vesicles in disease
- Surgical site infection prevention
- Management of metastatic bone disease
- Pleural and Pulmonary Diseases
- Phagocytosis and Immune Regulation
- Inflammatory Biomarkers in Disease Prognosis
- Renal and related cancers
- Congenital Diaphragmatic Hernia Studies
Charles University
2022-2025
University Hospital in Motol
2022-2025
Leibniz Association
2024
Leibniz Institute for Immunotherapy
2024
Chungbuk National University
2024
TU Dresden
2022-2024
Carl Gustav Carus-Institut
2024
Masaryk Memorial Cancer Institute
2024
University Hospital Carl Gustav Carus
2022-2024
Konkuk University
2024
Abstract Macrophages represent heterogeneous cell population with important roles in defence mechanisms and homoeostasis. Tissue macrophages from diverse anatomical locations adopt distinct activation states. M1 M2 are two polarized forms of mononuclear phagocyte vitro differentiation phenotypic patterns functional properties, but vivo, there is a wide range different macrophage phenotypes between depending on the microenvironment natural signals they receive. In human infections, pathogens...
Abstract Breast cancer is a highly heterogeneous disease. Its intrinsic subtype classification for diagnosis and choice of therapy traditionally relies on the presence characteristic receptors. Unfortunately, this often not sufficient precise prediction disease prognosis treatment efficacy. The N-glycan profiles 145 tumors 10 healthy breast tissues were determined using Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry. tumor samples classified into Mucinous,...
Dendritic cells (DCs) play a key role in the orchestration of antitumor immunity. Activated DCs efficiently enhance effects mediated by natural killer and T lymphocytes. Conversely, tolerogenic essentially contribute to an immunosuppressive tumor microenvironment. Thus, can profoundly influence progression clinical outcome patients. To gain novel insights into human pancreatic ductal adenocarcinoma (PDAC), we explored frequency, spatial organization, significance conventional type 1 (cDC1s)...
Head and neck squamous cell carcinoma (HNSCC) is one of the most common tumor entities worldwide, with human papillomavirus (HPV) infection contributing to cancer development. Conventional therapies achieve only limited efficiency, especially in recurrent or metastatic HNSCC. As immune landscape decisively impacts survival patients treatment efficacy, this study comprehensively investigated immunological microenvironment (TME) its association patient outcome, special focus on several...
Radiotherapy can be both immunosuppressive and immunostimulatory. Radiation-induced lymphopenia (RIL) is an ongoing challenge in cancer treatment. We investigated weekly changes the absolute lymphocyte count (ALC) during proton radiotherapy, evaluating effects of different dosage, fractionation schedules, pelvic node irradiation (PNI). Prostate patients were prospectively chosen for this study, due to their relatively homogenous treatment plans. Treatment protocols categorized into three...
Abstract Purpose Immune checkpoint inhibitors (ICIs) dramatically changed the prognosis of patients with NSCLC. Unfortunately, a reliable predictive biomarker is still missing. Commonly used biomarkers, such as PD-L1, MSI, or TMB, are not quite accurate in predicting ICI efficacy. Methods In this prospective observational cohort study, we investigated role erythrocytes, thrombocytes, innate and adaptive immune cells, complement proteins (C3, C4), cytokines from peripheral blood 224 stage...
Abstract Purpose The CD47 molecule, often referred to as the “do not eat me” signal, is frequently overexpressed in tumor cells. This signaling pathway limits phagocytosis by macrophages. Our objective was determine abundance various soft tissue sarcomas (STS) investigate whether it could serve a potential evasion mechanism for Additionally, we aimed assess prognostic value of expression examining its association with different clinicopathological factors. study elucidate significance...
IL-32 expression is important for pathogen clearance but detrimental in chronic inflammation, autoimmunity, and cancer. T cells are major producers these diseases key mediators of tumor elimination also autoimmune destruction. However, their contribution to biology during immune responses hardly understood due several isoforms with divergent inflammatory properties. Here, we identified IL-32β as the predominant isoform various cell subsets healthy individuals breast cancer patients highest...
Purpose: The IMMUNOSARC trial combined an antiangiogenic agent (sunitinib) with a PD1 inhibitor (nivolumab) in advanced sarcomas. Here, we present the first correlative studies of soft-tissue sarcoma cohort enrolled this trial. Experimental Design: Formalin-fixed paraffin-embedded and peripheral blood samples were collected at baseline week 13. used for transcriptomics multiplex immunofluorescence, whereas multiplexed immunoassays. Flow cytometry Luminex assays performed to validate...
Chest wall sarcomas are rare and pose significant technical challenges in surgical management, particularly patients with advanced disease. In this study, we examined the extent of resection, reconstruction techniques, oncological outcomes chest soft tissue bone sarcomas.
<div>Purpose:<p>The IMMUNOSARC trial combined an antiangiogenic agent (sunitinib) with a PD1 inhibitor (nivolumab) in advanced sarcomas. Here, we present the first correlative studies of soft-tissue sarcoma cohort enrolled this trial.</p>Experimental Design:<p>Formalin-fixed paraffin-embedded and peripheral blood samples were collected at baseline week 13. Formalin-fixed used for transcriptomics multiplex immunofluorescence, whereas multiplexed immunoassays. Flow...
<p>Supplementary Figure S2. Correlation between progression-free survival (PFS) and the dynamic increase in cytologic markers. A) CD3; B) CD4; C) CD8; D) CD20; E) CD66b; F) CD68 G) CD138. PFS cut-off was defined as 9.4 months calculated by MAXSTAT. Significance p<0.05 Wilcoxon paired test.</p>
<p>Supplementary Figure S4. Correlation between progression-free survival (PFS) and the dynamic alteration in expression of additional soluble proteins plasma samples. Samples were collected at baseline week 13 (Cycle 3, day 1). PFS cut-off was defined as 7.2 months calculated by MAXSTAT. Significance p<0.05 Wilcoxon paired test. Concentration is represented log2 (pg/mL).</p>
<p>Supplementary Figure S5. CXCL10 protein expression in cell culture supernatant. The levels of soluble were measured after anti-CD3/CD28 stimulation and anti-PD-1 treatment PBMC cultures (A) tumor multi-cultures (B). Significance was defined as p<0.05 (Students t-test).</p>
<p>Supplementary Figure S1. Consort diagram. Number of samples included in the correlative studies HTG transcriptomics, multiplexed immunoassays and immune-phenotyping.</p>
<p>Supplementary Figure S3. Dynamic modulation of soluble factors in paired plasma samples. Samples were collected at baseline and week 13 (Cycle 3, day 1). Significance was defined as p<0.05 calculated by Wilcoxon test. Concentration is represented log2 (pg/mL).</p>