A5064129294- interferon and immune responses
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- Vibrio bacteria research studies
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Poxvirus research and outbreaks
- Escherichia coli research studies
- COVID-19 Clinical Research Studies
- Ubiquitin and proteasome pathways
- RNA regulation and disease
- Parasitic Infections and Diagnostics
- Toxoplasma gondii Research Studies
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- Advanced biosensing and bioanalysis techniques
- Bacterial Infections and Vaccines
- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- Respiratory viral infections research
The Francis Crick Institute
2024-2025
Imperial College London
2013-2022
University of Cambridge
2018-2022
Royal Navy
2020
Medical Research Council
2013
Natural transformation is a dominant force in bacterial evolution by promoting horizontal gene transfer. This process may have devastating consequences, such as the spread of antibiotic resistance or emergence highly virulent clones. However, uptake and recombination foreign DNA are most often deleterious to competent species. Therefore, model naturally transformable Gram-negative bacteria, including human pathogen Neisseria meningitidis , evolved means preferentially take up homotypic...
Cryptosporidium is a common intestinal infection of vertebrates and significant threat to public health. Within the epithelial layer intestine, parasite invades replicates. Infected cells are readily detected under microscope by presence elongated microvilli, particularly around vacuole where resides. Here, we identify family virulence factors that exported into host cell during localise microvilli. We examine trafficking function most highly expressed member, MVP1, which appears control...
The transcription factors IRF3 and NF-κB are crucial in innate immune signalling response to many viral bacterial pathogens. However, mechanisms leading their activation remain incompletely understood. Viral RNA can be detected by RLR receptors, such as RIG-I MDA5, the dsRNA receptor TLR3. Alternatively, DExD-Box helicases DDX1-DDX21-DHX36 activate IRF3/NF-κB a TRIF-dependent manner independent of RIG-I, or Here, we describe DDX50, which shares 55.6% amino acid identity with DDX21,...
ABSTRACT The enteric pathogens enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. employ a type 3 secretion system (T3SS) to manipulate the host inflammatory response during infection. Previously, it has been reported that EPEC, in T3SS-dependent manner, induces an early proinflammatory through activation of NF-κB via extracellular signal-regulated kinases 1 2 (ERK1/2) protein kinase Cζ (PKCζ). However, infection not yet attributed effector. At later time points postinfection,...
NF-κB is a critical transcription factor in the innate immune response to infection and shaping adaptive immunity. The identification of host virus proteins that modulate induction immunological memory important for improving virus-based vaccine design efficacy. In viruses, expression BTB-BACK Kelch-like (BBK) restricted poxviruses conserved within them, indicating importance these medically viruses. Using vaccinia (VACV), smallpox vaccine, we report VACV BBK protein A55 dysregulates...
Vaccinia virus (VACV) encodes scores of proteins that suppress host innate immunity and many these target intracellular signalling pathways leading to activation inflammation. The transcription factor NF-κB plays a critical role in the response infection is targeted by viruses, including VACV 12 inhibitors interfere at different stages this pathway. Here we report C2 F3 are additional BTB-Kelch expressed early during infection, non-essential for replication, but affect outcome vivo . Using...
Introduction Immunoassays targeting different SARS-CoV-2-specific antibodies are employed for seroprevalence studies. The degree of variability between immunoassays anti-nucleocapsid (anti-NP; the majority) vs potentially neutralising anti-spike (including anti-receptor-binding domain; anti-RBD), particularly in mild or asymptomatic disease, remains unclear. Aims We aimed to explore anti-NP and anti-RBD antibody detectability following symptomatic SARS-CoV-2 infection analyse response...
The Cryptosporidium parasite is one of the leading causes diarrheal morbidity and mortality in children, adolescent infections are associated with chronic malnutrition. There no vaccines available for protection only drug approved treatment that has limited efficacy. A major barrier to developing new therapeutics a lack foundational knowledge biology, including which genes essential survival virulence. Here, we iteratively improve tools genetically manipulating develop targeted CRISPR-based...
ABSTRACT BTB-Kelch proteins are substrate-specific adaptors for cullin-3 (Cul3) RING-box based E3 ubiquitin ligases, which mediate protein ubiquitylation leading to proteasomal degradation. Vaccinia virus encodes three proteins, namely A55, C2 and F3. Viruses lacking A55 or demonstrate altered cytopathic effect in cultured cells pathology vivo . Previous studies show that the ectromelia orthologue of EVM150, interacts with Cul3 cells. We binds directly via its N-terminal BTB-BACK domain,...
Abstract The transcription factors IRF3 and NF-κB are crucial in innate immune signalling response to many viral bacterial pathogens. However, mechanisms leading their activation remain incompletely understood. Canonical RLR detection of RNA is dependent upon the receptors RIG-I, MDA5 TLR3. Alternatively, DExD-Box helicases DDX1-DDX21-DHX36 activate IRF3/NF-κB a TRIF-dependent manner independent or Here we describe DDX50, which shares 55.6% amino acid identity with DDX21, as component dsRNA...
Introduction Serological testing can augment delayed case identification programmes for Severe Acute Respiratory Syndrome coronoravirus-2 (SARS-CoV-2). Immunoassays employ anti-nucleocapsid (anti-NP; the majority) or potentially neutralising anti-spike (including anti-receptor binding domain; anti-RBD) antibody targets, yet correlation between assays and variability arising from disease symptomatology remains unclear. We explore these possibly differential immune responses across spectrum....