A5028392008- Sarcoma Diagnosis and Treatment
- Cardiac tumors and thrombi
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Vascular Tumors and Angiosarcomas
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Angiogenesis and VEGF in Cancer
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- Carcinogens and Genotoxicity Assessment
- Gastrointestinal Tumor Research and Treatment
- Plant Genetic and Mutation Studies
- Genetic Mapping and Diversity in Plants and Animals
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Ion Channels and Receptors
- Oral health in cancer treatment
- Evolution and Genetic Dynamics
- Cancer, Stress, Anesthesia, and Immune Response
- Chemotherapy-induced organ toxicity mitigation
- Tumors and Oncological Cases
- RNA Research and Splicing
- Neurofibromatosis and Schwannoma Cases
- ATP Synthase and ATPases Research
Universidad Autónoma de Madrid
2018-2024
Hospital Universitario Fundación Jiménez Díaz
2022-2024
Universidad Rey Juan Carlos
2024
Centro de Investigaciones Biológicas Margarita Salas
2016-2023
Homologous recombination (HR) is the principal mechanism of DNA repair acting during meiosis and fundamental for segregation chromosomes increase genetic diversity. Nevertheless, non-homologous end joining (NHEJ) mechanisms can also act meiosis, mainly in response to exogenously-induced damage late stages first meiotic prophase. In order better understand relationship between these two pathways, we studied male mouse after gamma radiation. We clearly discerned types responses immediately...
During meiotic prophase I, homologous chromosomes pair, synapse and recombine in a tightly regulated process that ensures the generation of genetically variable haploid gametes. Although mechanisms underlying cell division have been well studied model species, our understanding dynamics I non-traditional mammals remains its infancy. Here, we reveal key features previously uncharacterised marsupial species (the tammar wallaby fat-tailed dunnart), plus mouse opossum, with focus on sex...
The uncoupling protein UCP2 is a mitochondrial carrier for which transport activity remains controversial. physiological contexts in expressed have led to the assumption that, like UCP1, it uncouples oxidative phosphorylation and thereby reduces generation of reactive oxygen species. Other reports involved Warburg effect, results showing that catalyzes export matrix C4 metabolites facilitate glutamine utilization suggest could be metabolic adaptations required cell proliferation. We examined...
Abstract HMGA1 is a structural epigenetic chromatin factor that has been associated with tumor progression and drug resistance. Here, we reported the prognostic/predictive value of for trabectedin in advanced soft-tissue sarcoma (STS) effect inhibiting or mTOR downstream pathway activity. The expression was assessed cohort 301 STS patients at mRNA (n = 133) protein level 272), by HTG EdgeSeq transcriptomics immunohistochemistry, respectively. silencing on activity gene profiling measured...
Sex chromosomes of eutherian mammals are highly different in size and gene content, share only a small region homology (pseudoautosomal region, PAR). They thought to have evolved through an addition-attrition cycle involving the addition autosomal segments sex their subsequent differentiation. The events that drive this process difficult investigate because almost all at very advanced stage Here, we taken advantage recent translocation autosome both African pygmy mouse Mus minutoides, which...
Abstract Background Cisplatin is a highly emetogenic antineoplastic drug and induces peripheral neuropathy when given in cycles. Granisetron, 5‐HT 3 antagonist, clinically used to prevent chemotherapy‐induced nausea/emesis abdominal pain irritable bowel syndrome. The effects of cisplatin on visceral sensitivity those granisetron the context cancer chemotherapy are not well known. Methods Adult male Wistar rats received two intraperitoneal injections 30 minutes apart: (1 mg kg −1 )/vehicle (6...
Cisplatin, a chemotherapeutic drug, is known for causing gastrointestinal disorders and neuropathic pain, but its impact on visceral sensitivity unclear. Monosodium glutamate (MSG) has been shown to improve dysmotility pain induced by cisplatin in rats. This study aimed determine if repeated treatment alters whether dietary MSG can prevent these changes. Male Wistar HAN rats were treated with saline or (2 mg/kg/week, ip) 5 weeks, intracolonic mechanical stimulation was recorded after the...
X and Y chromosomes in mammals are different size gene content due to an evolutionary process of differentiation degeneration the chromosome. Nevertheless, these usually share a small region homology, pseudoautosomal (PAR), which allows them perform partial synapsis undergo reciprocal recombination during meiosis, ensures their segregation. However, some mammalian species PAR has been lost, challenges pairing segregation sex meiosis. The African pygmy mouse Mus mattheyi shows completely...
In eutherian mammals, hundreds of programmed DNA double-strand breaks (DSBs) are generated at the onset meiosis. The damage response is then triggered. Although dynamics this well studied in recent findings have revealed different patterns signaling and repair marsupial mammals. To better characterize these differences, here we analyzed synapsis chromosomal distribution meiotic DSBs markers three species ( Thylamys elegans , Dromiciops gliorides, Macropus eugenii ) that represent South...
Abstract Background Food and diet are central issues for proper functioning of the cardiovascular (CV) system gastrointestinal (GI) tract. We hypothesize that different types dietary FAs affect CV parameters as well GI motor function visceral sensitivity. Methods Male Wistar rats were fed with control (CTRL), supplemented 7% soybean oil (SOY), SOY + 3.5% virgin coconut (COCO), evening primrose (EP) 4 weeks. The content insoluble fiber in CTRL was higher than SOY, COCO, or EP. Body weight...
Although the overall survival of advanced soft-tissue sarcoma (STS) patients has increased in recent years, median progression-free is lower than 5 months, meaning that there an unmet need this population. Among second-line treatments for STS, eribulin anti-microtubule agent been approved liposarcoma. Here, we tested combination with gemcitabine preclinical models L-sarcoma. The effect cell viability was measured by MTS and clonogenic assay. Cell cycle profiling studied flow cytometry, while...
ABSTRACT Sex chromosomes of eutherian mammals are highly different in size and gene content, share only a small region homology (pseudoautosomal region, PAR). They thought to have evolved through an addition-attrition cycle involving the addition autosomal segments sex their subsequent differentiation. The events that drive this process difficult investigate because most at very advanced stage Here, we taken advantage recent translocation autosome both African pygmy mouse Mus minutoides ,...
Background Gastrointestinal stromal tumours (GISTs) are prevalent mesenchymal of the gastrointestinal tract, commonly exhibiting structural variations in KIT and PDGFRA genes. While mutational profiling somatic is well described, genes behind susceptibility to develop GIST not yet fully discovered. This study explores genomic landscape two primary cases, aiming identify shared germline pathogenic variants shed light on potential key players tumourigenesis. Methods Two patients with distinct...
Purpose: The IMMUNOSARC trial combined an antiangiogenic agent (sunitinib) with a PD1 inhibitor (nivolumab) in advanced sarcomas. Here, we present the first correlative studies of soft-tissue sarcoma cohort enrolled this trial. Experimental Design: Formalin-fixed paraffin-embedded and peripheral blood samples were collected at baseline week 13. used for transcriptomics multiplex immunofluorescence, whereas multiplexed immunoassays. Flow cytometry Luminex assays performed to validate...
Background: Alveolar soft-part sarcoma (ASPS) is a rare tumor driven by the ASPSCR1-TFE3 fusion protein, with propensity for metastasis. Prognostic factors remain poorly understood, and traditional chemotherapies are largely ineffective. Recent interest lies in immune checkpoint inhibitors (ICIs), yet predictive biomarkers treatment response lacking. Previous studies have shown promising results ICIs ASPS, indicating need further investigation into associated response. Objectives: To...
<div>Purpose:<p>The IMMUNOSARC trial combined an antiangiogenic agent (sunitinib) with a PD1 inhibitor (nivolumab) in advanced sarcomas. Here, we present the first correlative studies of soft-tissue sarcoma cohort enrolled this trial.</p>Experimental Design:<p>Formalin-fixed paraffin-embedded and peripheral blood samples were collected at baseline week 13. Formalin-fixed used for transcriptomics multiplex immunofluorescence, whereas multiplexed immunoassays. Flow...
<p>Supplementary Figure S2. Correlation between progression-free survival (PFS) and the dynamic increase in cytologic markers. A) CD3; B) CD4; C) CD8; D) CD20; E) CD66b; F) CD68 G) CD138. PFS cut-off was defined as 9.4 months calculated by MAXSTAT. Significance p<0.05 Wilcoxon paired test.</p>
<p>Supplementary Figure S4. Correlation between progression-free survival (PFS) and the dynamic alteration in expression of additional soluble proteins plasma samples. Samples were collected at baseline week 13 (Cycle 3, day 1). PFS cut-off was defined as 7.2 months calculated by MAXSTAT. Significance p<0.05 Wilcoxon paired test. Concentration is represented log2 (pg/mL).</p>