A5078151608- Reproductive Biology and Fertility
- DNA Repair Mechanisms
- Sperm and Testicular Function
- Carcinogens and Genotoxicity Assessment
- Plant Genetic and Mutation Studies
- Animal Genetics and Reproduction
- Urologic and reproductive health conditions
- Nuclear Structure and Function
- Testicular diseases and treatments
- Genomics and Chromatin Dynamics
Erasmus MC
2018-2020
Erasmus University Rotterdam
2018-2020
Universidad Autónoma de Madrid
2018-2019
Abstract STUDY QUESTION What is the diagnostic potential of next generation sequencing (NGS) based on a ‘mouse azoospermia’ gene panel in human non-obstructive azoospermia (NOA)? SUMMARY ANSWER The performance genes associated with mouse was relatively successful idiopathic NOA patients and allowed discovery two novel involved due to meiotic arrest. WHAT IS KNOWN ALREADY largely heterogeneous clinical entity, which includes different histological pictures. In large proportion NOA, aetiology...
Homologous recombination (HR) is the principal mechanism of DNA repair acting during meiosis and fundamental for segregation chromosomes increase genetic diversity. Nevertheless, non-homologous end joining (NHEJ) mechanisms can also act meiosis, mainly in response to exogenously-induced damage late stages first meiotic prophase. In order better understand relationship between these two pathways, we studied male mouse after gamma radiation. We clearly discerned types responses immediately...
During mammalian meiotic prophase, homologous chromosomes connect through the formation of synaptonemal complex (SC). SYCP3 is a component lateral elements SC. We have generated transgenic mice expressing N- or C-terminal fluorescent-tagged (mCherry-SYCP3 (CSYCP) and SYCP3-mCherry (SYCPC)) to study SC dynamics chromosome movements in vivo. Neither transgene rescued aberrations Sycp3 knockouts, but CSYCP could form short axial element-like structures absence endogenous SYCP3. On wild-type...
AbstractObjectiveTo establish which meiotic checkpoints are activated in males with severe spermatogenic impairment to improve phenotypic characterization of defects.DesignRetrospective observational study.SettingUniversity medical center research laboratory and andrology clinic.Patient(s)Forty-eight patients confirmed (Johnsen scores 3–6) 15 controls score 10).Intervention(s)None.Main Outcome Measure(s)Quantitative assessment immunofluorescent analyses specific markers determine entry,...
Abstract Homologous recombination (HR) is the principal mechanism of DNA repair acting during meiosis and fundamental for segregation chromosomes increase genetic diversity. Nevertheless, non-homologous end joining (NHEJ) mechanisms also act meiosis, mainly in response to exogenously-induced damage late stages first meiotic prophase. In order better understand relationship between these two pathways, we studied male mouse after gamma radiation. We clearly discerned types responses...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)