A5022371569- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Plant Genetic and Mutation Studies
- Microtubule and mitosis dynamics
- Sperm and Testicular Function
- Cell death mechanisms and regulation
- Genetic Mapping and Diversity in Plants and Animals
- Reproductive Biology and Fertility
- Neonatal Respiratory Health Research
- Prenatal Screening and Diagnostics
- Gestational Trophoblastic Disease Studies
- CRISPR and Genetic Engineering
- Congenital Diaphragmatic Hernia Studies
- Asthma and respiratory diseases
- Carcinogens and Genotoxicity Assessment
- Respiratory viral infections research
University of Chile
1999-2022
The nuclear architecture of meiotic prophase spermatocytes is based on higher-order patterns spatial associations among chromosomal domains from different bivalents. depends the chromosome characteristics and consequently prone to modification by rearrangements. In this work, we consider Mus domesticus with diploid number 2n = 40, all telocentric, investigate a possible ancestral due emergence derived Rb chromosomes, which may be present in homozygous or heterozygous condition. 40...
Rb translocations are chromosomal rearrangements frequently found in natural populations of the house mouse Mus musculus domesticus. The standard diploid karyotype consisting 40 telocentric chromosomes may be reduced by emergence metacentric chromosomes. Multiple simple heterozygotes form trivalents exhibiting higher anaphase nondisjunction frequency and consequently number unbalanced gametes than normal males. This work will attempt to establish whether frequencies aneuploidy observed...
We investigated whether apoptotic spermatocytes from the mouse Mus m. domesticus presented alterations in chromosomal synapses and DNA repair. To enrich for spermatocytes, scrotum's temperature was raised by partially exposing animals 15 min to a 42ºC water bath. Spermatocytes initial apoptosis were identified situ detecting activated Caspase-9. SYCP1 SYCP3 markers evaluating or structure of synaptonemal complexes Rad51 γH2AX repair chromatin remodeling. Apoptotic concentrated spermatogenic...
The size and shape of the chromosomes, as well chromosomal domains that compose them, are determinants in distribution interaction between bivalents within nucleus spermatocytes prophase I meiosis. Thus nuclear architecture characteristic karyotype a species can be modified by changes such Rb chromosomes. In this study we analysed meiotic organization heterozygous from Mus musculus domesticus 2n=26, synaptic configuration hexavalent formed dependent chromosomes Rbs 6.16, 16.10, 10.15, 15.17...
The nuclear architecture of meiotic prophase spermatocytes is based on higher-order patterns spatial associations among chromosomal domains and consequently prone to modification by rearrangements. We have shown that modified in Robertsonian (Rb) homozygotes Mus domesticus. In this study we analyse the synaptic configuration quadrivalents formed mice double heterozygotes for dependent Rb chromosomes: Rbs 11.16 16.17. Electron microscope spreads 60 pachytene from four animals domesticus 2n =...
We studied and compared the nucleolar expression or nucleoli from specific bivalents in spermatocytes of standard Mus musculus domesticus 2n = 40, Robertsonian (Rb) homozygotes 24 heterozygotes 32. analyzed 200 nuclear microspreads each chromosome spermatocyte karyotype, using FISH to identify bivalents, immunofluorescence for both fibrillarin nucleolus synaptonemal complex DAPI heterochromatin. There was all chromosomal conditions studied. By bivalent, quantitative relative higher bivalent...
Robertsonian translocation is the most common chromosomal rearrangement in mammals, and represents type of change that effectively contributes to speciation natural populations. Rb translocations involve double-strand DNA breaks at centromere level two telocentric chromosomes, followed by repair ligation respective long arms, creating a metacentric chromosome. Many different races have been described Mus musculus domesticus show reduced chromosome numbers due presence chromosomes. The...