A5004826274- Electrochemical Analysis and Applications
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Synthesis and Properties of Aromatic Compounds
- Free Radicals and Antioxidants
- Analytical Chemistry and Sensors
- DNA and Nucleic Acid Chemistry
- Photochromic and Fluorescence Chemistry
- Phytochemicals and Antioxidant Activities
- Trace Elements in Health
- Silymarin and Mushroom Poisoning
- Berberine and alkaloids research
- Crystallization and Solubility Studies
- Synthesis and bioactivity of alkaloids
- Analytical Chemistry and Chromatography
- X-ray Diffraction in Crystallography
- Chromatography in Natural Products
- Advanced Chemical Sensor Technologies
- Sulfur Compounds in Biology
- Cannabis and Cannabinoid Research
- Molecular Junctions and Nanostructures
- Drug Transport and Resistance Mechanisms
- Pharmacogenetics and Drug Metabolism
- Biotin and Related Studies
- Ion Transport and Channel Regulation
Palacký University Olomouc
2016-2025
Czech Academy of Sciences, Institute of Biophysics
2003-2022
Czech Academy of Sciences, Institute of Microbiology
2017
Universitat de València
2017
University Hospital Olomouc
2014-2016
Faculty (United Kingdom)
2014-2016
Czech Academy of Sciences, Institute of Chemical Process Fundamentals
2014
GTx (United States)
2012
Mendel University in Brno
2003-2009
Czech Academy of Sciences
2002-2009
Most research on American cranberry in the prevention of urinary tract infection (UTI) has used juices. The spectrum components juice is limited. This study tested whether whole fruit powder (proanthocyanidin content 0.56%) could prevent recurrent UTI 182 women with two or more episodes last year. Participants were randomized to a (n = 89) placebo group 93) and received daily 500 mg for 6 months. number diagnoses was counted. intent-to-treat analyses showed that group, UTIs significantly...
The basic nutritional aspects and parameters of freshwater marine algal food products are described. Blue-green algae (Spirulina pacifica, S. platensis), green (Chlorella pyrenoidosa), red (Palmaria palmata, Porphyra tenera), brown (Eisenia bicyclis, Hizikia fusiformis, Laminaria japonica, Undaria pinnatifida) were used for this purpose. ash content, total nitrogen, dietary fibers, in vitro digestibility the above-mentioned species studied. contents amounted to 8-11% (for freshwater) 9-33%...
The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, extracts of transgenic maize. detection limits denatured native 6 pM 120 nM, respectively. results demonstrated that streptavidin/avidin is sensitive specific method for quantifying biological samples such as foods tissue extracts,...
An electrospray ionization mass spectrometric (ESI-MS) determination of glutathione (GSH), a sulfur-containing tripeptide (γ-Glu-Cys-Gly) with regulation and detoxication functions in metabolisms most living organisms, from nanomolar to micromolar levels is described. A hydrophilic interaction chromatography (HILIC) an isocratic elution using mobile phase containing acetonitrile aqueous 0.00005% solution trifluoroacetic acid (60/40%, v/v) was applied for the separation GSH. The peptide...
The natural flavonoid quercetin is a low affinity ligand of the aryl hydrocarbon receptor (AhR), transcription factor regulating expression cytochrome P450 (CYP) 1A enzymes. This study examined ability quercetin, isoquercitrin (quercetin-3-O-glucoside), rutin (quercetin-3-O-rutinoside) and taxifolin (dihydroquercetin) to activate AhR induce CYP1A1 in human hepatoma HepG2 cells. Gene reporter assays showed that significantly activated triggered after 24 h exposure. These effects were,...
Abstract Herein we demonstrate the synthesis of a helicene‐based imidazolium salt. The salt was prepared by starting from racemic 2‐methyl[6]helicene, which undergoes radical bromination to yield 2‐(bromomethyl)[6]helicene. Subsequent treatment with 1‐butylimidazole leads corresponding 1‐butyl‐3‐(2‐methyl[6]helicenyl)‐imidazolium bromide. subsequently characterized using NMR spectroscopy and X‐ray analysis, various optical spectrometric techniques, computational chemistry tools. Finally,...
Abstract Objective The aim of this study was to: (1) evaluate the anti‐inflammatory effects cannabidiol (CBD) on primary cultures human gingival fibroblasts (HGFs) and (2) to clinically monitor effect CBD in subjects with periodontitis. Background use phytocannabinoids is a new approach treatment widely prevalent periodontal disease. Materials Methods Cannabinoid receptors were analyzed by western blot interleukin production detected using enzyme immunoassay. Activation Nrf2 pathway studied...
The potentials of electrochemical processes in ideal aqueous media are related to the potential a normal hydrogen electrode (NHE). However, non-ideal media, metallocene redox couple used as reference. Such measurements with free solution complicated by adsorption and mass transport phenomena solvation effects. Herein, platinum an anchored ferrocene moiety (Pt,Fc) was for cyclic voltammetric ferrocene/ferrocenium (Fc/Fc+) transformation not only non-aqueous but, first time, solutions well....
Nitro-fatty acids (NO₂FAs) are endogenously produced electrophiles and NRF2 activators with therapeutic potential. In this work, we developed a synthetic protocol combining Henry reaction base-promoted β-elimination, yielding ultra-pure regio/stereoisomers of nitro-stearic (NO₂SA), nitro-oleic (NO₂OA), conjugated/bis-allylic nitro-linoleic (NO₂LA) acids. These were tested for pathway activation in bone marrow cells under different oxygen conditions. We observed that 9- 10-NO₂OA, 10-NO₂LA...
Quercetin and gallic acid are natural activators of the transcription factor Nrf2, which regulates expression many cytoprotective enzymes including heme oxygenase-1 (HO-1). We developed procedures for synthesis monogalloyl esters quercetin taxifolin (dihydroquercetin), namely, 3-O-galloylquercetin 7-O-galloyltaxifolin, examined their effect on Nrf2 pathway in RAW264.7 cells. Unlike free acid, derivatives isoquercitrin (quercetin-3-O-β-d-glucoside) had no HO-1. In contrast,...
In electrochemical sensing, a number of voltammetric or amperometric curves are obtained which subsequently processed, typically by evaluating peak currents and potentials wave heights half-wave potentials, frequently after background correction. Transformations data can help to extract specific information, e.g., the transferred electrons, reveal aspects studied system, contribution adsorption phenomena. this communication, we introduce LabView-based software package, ‘eL-Chem Viewer’, is...