A5006668266- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Sugarcane Cultivation and Processing
- Plant Parasitism and Resistance
- Infections and bacterial resistance
- Polysaccharides and Plant Cell Walls
- Banana Cultivation and Research
- Enterobacteriaceae and Cronobacter Research
- Plant pathogens and resistance mechanisms
- Cassava research and cyanide
- RNA and protein synthesis mechanisms
- Enzyme Catalysis and Immobilization
- Microbial Applications in Construction Materials
- Chromium effects and bioremediation
- Wastewater Treatment and Nitrogen Removal
- Biofuel production and bioconversion
- Antibiotic Resistance in Bacteria
- Polyamine Metabolism and Applications
- Nematode management and characterization studies
Sorbonne Université
2008-2023
Institut d'écologie et des sciences de l'environnement de Paris
2015-2023
Centre National de la Recherche Scientifique
1994-2023
Institut de Recherche pour le Développement
2015-2023
Université Paris-Est Créteil
2017-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2023
AgroParisTech
2008-2018
Université Claude Bernard Lyon 1
1997-2018
Biologie et Génétique des Interactions Plante-Parasite
2011-2017
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015
Alcaligenes eutrophus strain CH34, which was isolated as a bacterium resistant to cobalt, zinc, and cadmium ions, shares with A. H16 the ability grow lithoautotrophically on molecular hydrogen, form cytoplasmic NAD-reducing membrane-bound hydrogenase, most metabolic attributes; however, it does not fructose. Strain CH34 contains two plasmids, pMOL28 (163 kilobases) specifying nickel, mercury, cobalt resistance pMOL30 (238 cadmium, resistance. The plasmids are self-transmissible in homologous...
Erwinia chrysanthemi produced several pectate lyases (EC 4.2.2.2) and endocellulases 3.2.1.4) which were largely secreted into the culture medium. Mutants deficient in secretion mechanism for these enzymes obtained by chemical insertion mutagenesis. Further study of one such mutant revealed that both enzyme activities retained simultaneously within periplasmic space.
Five transcription units of the Pseudomonas solanacearum hrp gene cluster are required for secretion HR-inducing PopA1 protein. The nucleotide sequences two these, 1 and 3, have been reported. Here, we present sequence three other units, 2, 4 7, which together predicted to code 15 genes. This brings total number Hrp proteins encoded by these five 20, including HrpB, positive regulatory protein, HpaP, is apparently not plant interactions. Among 18 proteins, eight belong protein families...
A pLAFR3 cosmid clone designated pVir2 containing a 25-kilobase (kb) DNA insert was isolated from wild-type Pseudomonas solanacearum GMI1000 genomic library. This shown to complement all but one of the nine Tn5-induced mutants which have been after random mutagenesis and lost both pathogenicity toward tomato ability induce hypersensitive reaction (HR) on tobacco (hrp mutants). The is colinear with genome provides restoration HR-inducing when transferred into several hrp mutants, failed...
Peptide methionine sulfoxide reductase (MsrA), which repairs oxidized proteins, is present in most living organisms, and the cognate structural gene belongs to so-called minimum set [Mushegian, A. R. & Koonin, E. V., (1996) Proc. Natl. Acad. Sci. USA 93, 10268–10273]. In this work, we report that MsrA required for full virulence of plant pathogen Erwinia chrysanthemi . The following differences were observed between wild-type a − mutant: ( i ) mutant was more sensitive oxidative stress;...
In addition to the important role of abscisic acid (ABA) in abiotic stress signalling, basal and high ABA levels appear have a negative effect on disease resistance. Using ABA-deficient sitiens tomato (Solanum lycopersicum) mutant different application methods exogenous ABA, we demonstrated influence this plant hormone progression Erwinia chrysanthemi. This necrotrophic pathogenic bacterium is responsible for soft rot many species, causing maceration symptoms mainly due production secretion...
Dickeya dadantii is a plant-pathogenic enterobacterium responsible for the soft rot disease of many plants economic importance. We present here sequence strain 3937, widely used as model system research on molecular biology and pathogenicity this group bacteria.
Summary D ickeya dadantii is a plant pathogen that secretes cell wall‐degrading enzymes ( CWDE ) are responsible for soft‐rot symptoms. Virulence genes expressed in concerted manner and culminate when bacterial multiplication slows. We identify 25 kb vfm cluster required . production pathogenesis. The encodes proteins displaying similarities both with involved amino acid activation fatty biosynthesis. These suggest the direct of metabolite. Cell‐free supernatant from wild‐type strain...
Strains 2B12T, FVG1-MFV-O17 and FVG10-MFV-A16 were isolated from fresh water samples collected in Asia Europe. The nucleotide sequences of the gapA barcodes revealed that all three strains belonged to same cluster within genus Dickeya. Using 13 housekeeping genes (fusA, rpoD, rpoS, glyA, purA, groEL, gapA, rplB, leuS, recA, gyrB, infB secY), multilocus sequence analysis confirmed existence a new clade. When genome these isolates other Dickeya species compared, silico DNA-DNA hybridization...
As in many other Gram‐negative plant pathogenic bacteria, the Ralstonia solanacearum hrp genes are involved production of a type III secretion apparatus that allows translocation PopA protein to external medium. Here, we show also biogenesis pili mainly composed HrpY protein. These produced at one pole bacterium and released into medium where they can form very long straight bundles. An hrpY mutant is defective pilus production, impaired interactions with plants but not attachment cells.
The development of competence allowing natural transformation Ralstonia solanacearum was found to occur during exponential growth and not in response any excreted factors. Linear DNAs were effectively integrated by recombination requiring a minimum 50 bp homologous DNA. Therefore, DNA from other genera species ineffective.
Pathogenicity of the enterobacterium Erwinia chrysanthemi (Dickeya dadantii), causative agent soft-rot disease in many plants, is a complex process involving several factors whose production subject to temporal regulation during infection. PecS transcriptional regulator that controls various virulence factors. Here, we used microarray analysis define regulon and demonstrated notably regulates wide range genes could be linked pathogenicity group concerned with evading host defenses. Among...
Plasmid pULB113 (RP4::mini-Mu), which contains the mini-Mu transposon, promoted both homologous and heterologous gene transfer from Pseudomonas fluorescens 6.2 Alcaligenes eutrophus CH34. Homologous in P. A. CH34 occurred at a frequency of 10(-4) to 10(-5), recombinants inherited unselected recessive markers, suggesting process chromosome mobilization. Loci involved autotrophic growth were among those transferred eutrophus. In heterospecific matings, markers eutrophus, Salmonella typhimurium...
Dickeya solani is an emerging pathogen that causes soft rot and blackleg diseases in several crops including Solanum tuberosum, but little known about its genomic diversity evolution. We combined Illumina PacBio technologies to complete the genome sequence of D. strain 3337 was used as a reference compare with 19 other genomes (including type IPO2222T) which were generated by technology. This population analysis highlighted unexpected variability among isolates since it led characterization...
Summary Pathogenicity of the phytopathogenic enterobacterium Erwinia chrysanthemi , causal agent soft rot disease on many plants, is a complex process involving several factors whose production regulated by complex, intertwined regulatory network. In this work we characterized GacA regulator, member GacS–GacA two‐component system, as global regulator which required for expression but not bacterial multiplication in planta during first stages plant infection. was shown to control cell...
The genus Dickeya comprises plant pathogens that cause diseases in a large range of economically important crops and ornamentals. Strains previously assigned to the species zeae are major attacking vital such as maize rice. They also frequently isolated from surface water. newly described oryzae is closely related D. members, so limit between two can be difficult define. In order clearly distinguish species, globally by term ‘ complex’, we sequenced genome four new water isolates compared...