A5061758836- Escherichia coli research studies
- Urinary Tract Infections Management
- Gut microbiota and health
- Urinary Bladder and Prostate Research
- Legume Nitrogen Fixing Symbiosis
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Plant Pathogenic Bacteria Studies
- Polysaccharides and Plant Cell Walls
- Vibrio bacteria research studies
- Protein Structure and Dynamics
- Plant Pathogens and Fungal Diseases
- Evolutionary Game Theory and Cooperation
- Plant Micronutrient Interactions and Effects
- Bacterial Genetics and Biotechnology
- Banana Cultivation and Research
- Geochemistry and Elemental Analysis
- Plant and Biological Electrophysiology Studies
- Antibiotic Resistance in Bacteria
- Microbial Fuel Cells and Bioremediation
- Glycosylation and Glycoproteins Research
- Plant-Microbe Interactions and Immunity
- Polymer-Based Agricultural Enhancements
- Cassava research and cyanide
- Genomics and Phylogenetic Studies
Indiana University Bloomington
2009-2022
Washington University in St. Louis
2013-2020
University of Wisconsin–Madison
2011
ABSTRACT Pectobacterium and Dickeya spp. are related broad-host-range entero-bacterial pathogens of angiosperms. A review the literature shows that these genera each cause disease in species from at least 35% angiosperm plant orders. The known host ranges partially overlap and, together, two 50% Notably, there no reported hosts for either genus eudicots clade magnoliids or eurosids II clades, although one three In addition, but not have been on a rosids unlike spp., many Poales species....
Comparative genomic and functional studies reveal that E. coli strains from women with recurrent urinary tract infection can move between gut without a fitness trade-off.
Risk for urinary tract infections is determined by a combination of host susceptibility determinants and diverse Escherichia coli phenotypes.
ABSTRACT Uropathogenic Escherichia coli (UPEC) is the primary etiological agent of over 85% community-acquired urinary tract infections (UTIs). Mouse models infection have shown that UPEC can invade bladder epithelial cells in a type 1 pilus-dependent mechanism, avoid TLR4-mediated exocytic process, and escape into host cell cytoplasm. The internalized clonally replicate biofilm-like intracellular bacterial communities (IBCs) thousands bacteria while avoiding many clearance mechanisms....
Dickeya dadantii is a plant-pathogenic enterobacterium responsible for the soft rot disease of many plants economic importance. We present here sequence strain 3937, widely used as model system research on molecular biology and pathogenicity this group bacteria.
Treatment of bacterial infections is becoming a serious clinical challenge due to the global dissemination multidrug antibiotic resistance, necessitating search for alternative treatments disarm virulence mechanisms underlying these infections. Uropathogenic Escherichia coli (UPEC) employs multiple chaperone-usher pathway pili tipped with adhesins diverse receptor specificities colonize various host tissues and habitats. For example, UPEC F9 specifically bind galactose or...
ABSTRACT Uropathogenic Escherichia coli (UPEC) is the primary cause of community-acquired urinary tract infections (UTIs). UPEC bind bladder using type 1 pili, encoded by fim operon in nearly all E. . Assembled pili terminate FimH adhesin, which specifically binds to mannosylated glycoproteins on epithelium. Expression regulated part phase-variable inversion genomic element containing fimS promoter, resulting phase ON (expressing) and OFF (nonexpressing) orientations. Type are essential for...
ABSTRACT The plant pathogen Agrobacterium tumefaciens encodes predicted iron-responsive regulators, Irr and RirA, that function in several other bacteria to control the response environmental iron levels. Deletion mutations of irr rirA , alone combination, were evaluated for their impact on cellular response. Growth was severely diminished Δ mutant under iron-limiting conditions, but reversed wild-type levels an mutant. level uncomplexed decreased, whereas exhibited elevated Sensitivity...
Evolutionary selection fine-tunes conformational ensembles of FimH for optimal bladder colonization by E. coli during UTI.
Urinary tract infections (UTI) are highly prevalent, a significant cause of morbidity and increasingly resistant to treatment with antibiotics. Females disproportionately afflicted by UTI: 50% all women will have UTI in their lifetime. Additionally, 20-40% these who an initial suffer recurrence some suffering frequent recurrences serious deterioration the quality life, pain discomfort, disruption daily activities, increased healthcare costs, few options other than long-term antibiotic...
Transition metals such as iron and manganese are crucial trace nutrients for the growth of most bacteria, functioning catalytic cofactors many essential enzymes. Dedicated uptake regulatory systems have evolved to ensure their acquisition growth, while preventing toxicity. Transcriptomic analysis iron- manganese-responsive regulons Agrobacterium tumefaciens revealed that there discrete networks respond changes in levels. Complementing earlier studies, iron-responsive gene network is quite...
Agrobacterium tumefaciens is a member of the Alphaproteobacteria that pathogenises plants and associates with biotic abiotic surfaces via single cellular pole. A. produces unipolar polysaccharide (UPP) at site surface contact. UPP production normally surface-contact inducible, but elevated levels second messenger cyclic diguanylate monophosphate (cdGMP) bypass this requirement. Multiple lines evidence suggest has central component. Using an derivative cdGMP mutationally disabled for other...
Urinary tract infections (UTIs) are predominantly caused by uropathogenic Escherichia coli (UPEC). UPEC pathogenesis requires passage through a severe population bottleneck involving intracellular bacterial communities (IBCs) that clonal expansions of single invading bacterium in urothelial superficial facet cell. IBCs occur only during acute pathogenesis. The bacteria form the founder develops into persistent extracellular infections. Only small fraction organisms proceed IBC cycle,...
Urinary tract infections (UTI) are highly prevalent, a significant cause of morbidity and increasingly resistant to treatment with antibiotics. Females disproportionately afflicted by UTI: 50% all women will have UTI in their lifetime. Additionally, 20-40% these who an initial suffer recurrence some suffering frequent recurrences serious deterioration the quality life, pain discomfort, disruption daily activities, increased healthcare costs, few options other than long-term antibiotic...
Positive selection in the two‐domain type 1 pilus adhesin FimH enhances Escherichia coli fitness urinary tract infection (UTI). Here, we report a comprehensive atomic‐level view of two‐state conformational ensembles solution, composed one low‐affinity tense ( T ) and multiple high‐affinity relaxed R conformations. Positively selected residues allosterically modulate equilibrium between these two states, each which engage mannose through distinct binding orientations. A variant that only...
Summary Agrobacterium tumefaciens , is a member of the Alphaproteobacteria that pathogenizes plants, and associates with biotic abiotic surfaces via single cellular pole. A. produces unipolar polysaccharide (UPP) at site surface contact. UPP production normally surface-contact inducible, but elevated levels second messenger cyclic diguanylate monophosphate (cdGMP) bypass this requirement. Multiple lines evidence suggest has central component. Using an derivative cdGMP mutationally disabled...