A5086551836- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Banana Cultivation and Research
- Biofuel production and bioconversion
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Carbohydrate Chemistry and Synthesis
- Plant nutrient uptake and metabolism
- Enzyme function and inhibition
- Bacterial biofilms and quorum sensing
- Sugarcane Cultivation and Processing
- Microbial Metabolic Engineering and Bioproduction
- Plant Disease Resistance and Genetics
- Glycosylation and Glycoproteins Research
- Infections and bacterial resistance
- bioluminescence and chemiluminescence research
- Enzyme Catalysis and Immobilization
- Plant pathogens and resistance mechanisms
- Vibrio bacteria research studies
- Microbial Metabolism and Applications
Centre National de la Recherche Scientifique
2013-2024
Université Claude Bernard Lyon 1
2013-2024
Laboratoire de Microbiologie, Adaptation et Pathogénie
2015-2024
Institut National des Sciences Appliquées de Lyon
2012-2024
Institut de Biologie et de Chimie des Protéines
1996-2008
GTx (United States)
2008
NTL Institute for Applied Behavioral Science
1999-2005
Laboratoire de Microbiologie et Génétique Moléculaires
1999-2004
University of Geneva
1990
Abstract Erwinia chrysanthemi causes soft‐rot diseases of many plants by secreting a battery enzymes which degrade the plant cell walls. We initiated proteomic analysis to create reference map E. secretome. Extracellular proteins were isolated from culture supernatants and resolved two‐dimensional electrophoresis. By mutants, Western blotting, matrix‐assisted laser desorption/ionization‐time flight (MALDI‐TOF) 55 spots representing 25 unique identified. In uninduced conditions, we identified...
To depolymerize plant pectin, the phytopathogenic enterobacterium Erwinia chrysanthemi produces a series of enzymes which include pectin-methyl-esterase encoded by pem gene and five isoenzymes pectate lyases genes pelA, pelB, pelC, pelD, pelE. We have constructed transcriptional fusions between pectinase promoters uidA gene, encoding beta-glucuronidase, to study regulation these E. individually. The transcription is dependent on many environmental conditions. All were induced pectic...
In Erwinia chrysanthemi 3937, pectate lyase activity mainly results from the cumulative action of five major isoenzymes, PelA to PelE. Comparison their amino acid sequences revealed two families, PelB-C and PelA-D-E. Molecular cloning permitted expression different pel genes in Escherichia coli isolation each Pel independently other isoenzymes. We used similar experimental conditions overproduce purify Pels a one-step chromatography method. analyzed some basic enzymatic properties these has...
Dickeya dadantii is a plant-pathogenic enterobacterium responsible for the soft rot disease of many plants economic importance. We present here sequence strain 3937, widely used as model system research on molecular biology and pathogenicity this group bacteria.
The genus Dickeya is an important group of plant pathogens that currently comprises eight recognized species. Although most isolates originated from infected cultivated plants, they have also been repeatedly isolated water. To better understand the natural diversity Dickeya, a survey was performed in small lakes surrounded by wetlands French region La Dombes. Several were obtained water or plants protected direct agricultural inputs. Sequencing gapA gene revealed five isolates, S12, S15,...
In the plant-pathogenic enterobacterium Erwinia chrysanthemi , almost all known genes involved in pectin catabolism are controlled by transcriptional regulator KdgR. this study, comparative genomics approach was used to analyse KdgR regulon completely sequenced genomes of eight enterobacteria, including Erw. and two Vibrio species. Application a signal recognition procedure complemented operon structure protein sequence analysis allowed identification new candidate regulon. Most these were...
Erwinia chrysanthemi 3937 secretes five major isoenzymes of pectate lyases encoded by the pel4, pelB, pelC, pelD, and pelE genes a set secondary lyases, two which, pelL pelZ, have been already identified. We cloned pelI gene, encoding ninth lyase E. 3937. The reading frame is 1,035 bases long, corresponding to protein 344 amino acids including typical amino-terminal signal sequence 19 acids. purified mature PelI has an isoelectric point about 9 apparent molecular mass 34 kDa. preference for...
Summary Erwinia chrysanthemi 3937 secretes five major isoenzymes of pectate lyases encoded by the pelA, pelB, pelC, pelD and pelE genes. Recently, a new set was identified in E. mutants deleted those pel We cloned pelL gene, encoding one these secondary 3937, from genomic bank strain The nucleotide sequence region containing gene determined. reading frame is 1275 bases long, corresponding to protein 425 amino acids including typical amino‐terminal signal 25 acids. Comparison acid sequences...
Plant pathogenic bacteria of the genera Dickeya and Pectobacterium are broad-host-range necrotrophs which cause soft-rot diseases in important crops. A metabolomic analysis, based on 13C-NMR spectroscopy, was used to characterize plant-bacteria interaction. Metabolic profiles revealed a decline plant sugars amino acids during infection concomitant appearance compound identified as 2,3-butanediol. Butanediol is major metabolite found macerated tissues various host plants. It accumulated...
The tol-pal genes are necessary for maintaining the outer-membrane integrity of Gram-negative bacteria. These were first described in Escherichia coli , and more recently several other species. They involved pathogenesis E. Haemophilus ducreyi Vibrio cholerae Salmonella enterica . role bacterial was investigated phytopathogenic enterobacterium Erwinia chrysanthemi assuming that this organism might be a good model such study. whole Er. region characterized. Tol-Pal proteins, except TolA,...
Erwinia chrysanthemi causes soft‐rot diseases of various plants by enzymatic degradation the pectin in plant cell walls. The structural complexity requires combined action several pectinases for its efficient breakdown. Three types have so far been identified E. : two methyl esterases (PemA, PemB), a polygalacturonase (PehX), and eight pectate lyases (PelA, PelB, PelC, PelD, PelE, PelL, PelZ, PelX). We report this paper analysis novel enzyme, acetyl esterase encoded paeY gene. No bacterial...
Summary The out genes of Erwinia chrysanthemi are required for the translocation across outer membrane pectate lyases and cellulases. We present characterization nucleotide sequence five cluster. products outS, B, C, D E have significant homology with Puts, proteins necessary to secretion pullulanase in Klebsiella pneumoniae. An open reading frame, outT , located between outB outC has no pul cluster but is involved secretion. cute, outD outE form an operon while constitute independent...
ABSTRACT Erwinia chrysanthemi is a phytopathogenic enterobacterium causing soft rot disease in wide range of plants. Osmoregulated periplasmic glucans (OPGs) are intrinsic components the gram-negative bacterial envelope. We cloned opgGH operon E. , encoding proteins involved glucose backbone synthesis OPGs, by complementation homologous locus mdoGH Escherichia coli . OpgG and OpgH show high level similarity with MdoG MdoH, respectively, mutations opgG or opgH gene abolish OPG synthesis. The...
In the phytopathogenic enterobacterium Erwinia chrysanthemi, catabolism of hexuronates is linked to degradation pectic polymers. We isolated Mu lac insertions in each gene hexuronate pathway and used genetic fusions with lacZ (the beta-galactosidase Escherichia coli) study regulation this pathway. Three independent regulatory genes (exuR, uxuR, kdgR) were found. Galacturonate glucuronate converted into 2-keto-3-deoxygluconate (KDG) by separate three-step pathways encoded uxaC, uxaB, uxaA...
The "family 9 polysaccharide lyase" pectate lyase L (Pel9A) from Erwinia chrysanthemi comprises a 10-coil parallel beta-helix domain with distinct structural features including an asparagine ladder and aromatic stack at novel positions within the superhelical structure. Pel9A has single high affinity calcium-binding site strikingly similar to "primary" described previously for family Pel1A lyases, there is strong evidence common second calcium ion that binds between enzyme substrate in...
Pseudomonas putida TOL plasmid pWW0 catabolic genes are clustered into two operons. The first, the upper operon, is controlled by xylR regulatory gene, whereas second, meta xylS gene. gene itself subjected to control xylR. In this study, we show that operons were poorly induced in cells growing at early-exponential-growth phase but strongly late-exponential-growth phase. We constructed fusions of four promoters, Pm (the promoter operon), Pu Ps gene), and Pr gene) with lacZ examined,...
Mutants of Erwinia chrysanthemi impaired in pectin degradation were isolated by chemical and Mu d(Ap lac) insertion mutagenesis. A mutation the kduD gene coding for 2-keto-3-deoxygluconate oxidoreductase prevented growth bacteria on polygalacturonate as sole carbon source. Analysis kduD::Mu insertions indicated that is either an or last a polycistronic operon. Some kduD-lac fusions which beta-galactosidase synthesis reflected expression. In all these fusions, activity was shown to be...
Bacteria from the genus Dickeya (formerly Erwinia chrysanthemi) are plant pathogens causing severe diseases in many economically important crops. A majority of strains responsible for potato disease Europe belong to a newly identified solani species. Although some ecological and epidemiological studies have been carried out, little is known about regulation D. virulence. The characterization four indicates significant differences their virulence on potato, although they genetically similar...
Bacteria belonging to the genera Dickeya and Pectobacterium are responsible for significant economic losses in a wide variety of crops ornamentals. During last years, increasing potato production have been attributed appearance solani. The D. solani strains investigated so far share genetic homogeneity, although different virulence levels were observed among various origins. purpose this study was investigate traits possibly related diverse by means comparative genomics. First, we developed...
The genus Dickeya is an important group of plant pathogens that currently comprises 10 recognized species. Although most isolates originated from infected cultivated plants, they are also isolated water. genomic sequence the Australian strain NCPPB 569 T clearly established its separation previously characterized average nucleotide identity and digital DNA–DNA hybridization values obtained by comparing with strains species were lower than 87 32 %, respectively, supporting delineation a new...