A5021602722- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Amphibian and Reptile Biology
- Rheology and Fluid Dynamics Studies
- Aquaculture disease management and microbiota
- Polymer crystallization and properties
- Antimicrobial Peptides and Activities
- Plant tissue culture and regeneration
- Turtle Biology and Conservation
- Plant Pathogenic Bacteria Studies
- Toxin Mechanisms and Immunotoxins
- Glycosylation and Glycoproteins Research
- Biofuel production and bioconversion
- Fern and Epiphyte Biology
- Genomics and Phylogenetic Studies
- Thermal Regulation in Medicine
- Polysaccharides and Plant Cell Walls
- Wound Healing and Treatments
- Sepsis Diagnosis and Treatment
- Parasite Biology and Host Interactions
- Synthesis and Biological Evaluation
- Block Copolymer Self-Assembly
- Mollusks and Parasites Studies
- Biomedical Ethics and Regulation
- bioluminescence and chemiluminescence research
Texas Tech University
2008-2024
Texas Tech University Health Sciences Center
2011
Dickeya dadantii is a plant-pathogenic enterobacterium responsible for the soft rot disease of many plants economic importance. We present here sequence strain 3937, widely used as model system research on molecular biology and pathogenicity this group bacteria.
Chain topology has a profound impact on the flow behavior of single macromolecules. For circular polymers, absence free ends results in unique chain architecture compared to linear or branched chains, thereby generating distinct molecular dynamics. Here, we report direct observation DNA dynamics transient and steady flows for sizes spanning range 25.0–114.8 kilobase pairs (kbp). Our show that longest relaxation times rings follow power-law scaling relation with weight differs from chains....
Batrachochytrium dendrobatidis is a fungal pathogen of amphibians that increasingly implicated as major cause large-scale mortalities amphibian species worldwide. Previous studies indicate motile zoospores B. colonize the keratinized tissues susceptible amphibians. Infections spread to adults and destruction epidermal tissue. In an effort understand how chytrid cues into its host we developed assay study chemotaxis in fungus. Here show exhibit positive movement toward variety attractants...
Bulk rheology measurements on concentrated monodisperse ring–linear DNA mixtures are reported for the first time. The entanglement behavior of (with 15 and 50% linear chain fractions, ϕLin) over a range total concentration, ϕTot, from ∼0.8 mg/mL (20C*) to 2 (50C*) is reported. A discussion current problems in ring dynamics/scaling included. concentration-dependent dynamics compared with 100% at same degree entanglement, Z. Dynamic oscillatory tests were conducted understand bulk rheological...
We have measured the dynamics of completely monodisperse (PDI = 1.0) ultrahigh-molecular-weight linear lambda (λ) DNA solutions as a function concentration. Due to very high molecular weight DNA, Mn Mw > 30 million g/mol, we were able study dynamic properties well-entangled systems even in dilute (low-concentration) conditions. report rheology by conducting oscillatory measurements well into entanglement regime (11 < C* 90), where is overlap The tests are reported good solvent Upon comparing...
Batrachochytrium dendrobatidis is a fungal pathogen of amphibians that increasingly implicated as major cause large-scale mortalities amphibian species worldwide. Previous studies indicate motile zoospores B. colonize the keratinized tissues susceptible amphibians. Infections spread to adults and destruction epidermal tissue. In an effort understand how chytrid cues into its host we developed assay study chemotaxis in fungus. Here show exhibit positive movement toward variety attractants...
Trauma patients (TPs) are highly susceptible to infections, which often lead sepsis. Among the numerous causative agents, Pseudomonas aeruginosa is especially important, as P. sepsis fatal. Understanding mechanism of its pathogenesis in bloodstream infections imperative; however, this has not been previously described. To examine effect trauma-induced changes blood on expression genes, we grew strain UCBPP-PA14 (PA14) samples from eight TPs and seven healthy volunteers (HVs). Compared with...
Individuals often experience ailments such as allergies, asthma and respiratory tract infections throughout the year. Weather reports include estimations of common allergens that can affect these individuals. To describe local 'atmospheric microbiome' in Lubbock, Texas, USA, we examined culturable fungal bacterial microbiome present air on calm dust storm days using internal transcribed spacer (ITS)-1 16S rRNA amplicon sequencing, respectively. While some types airborne fungi were frequently...
Abstract Pseudomonas aeruginosa is an opportunistic pathogen that uses malonate among its many carbon sources. We recently reported that, when grown in blood from trauma patients, P. expression of utilization genes was upregulated. In this study, we explored the role and contribution to virulence. grew strain PA14 M9 minimal medium containing (MM9) or glycerol (GM9) as a sole source assessed effect growth on quorum sensing, virulence factors, antibiotic resistance. Growth MM9, compared GM9,...
Uptake of [14C]galacturonic acid in Erwinia chrysanthemi was found to be stimulated during growth on pectin and its degradation products, saturated digalacturonic galacturonic acid. Cells isolated from macerated potato tissue also showed increased levels uptake activity for this molecule compared with those by glycerol-grown cells. an active process, it displayed saturation kinetics. An Escherichia coli transport mutant harboring the E. exuT gene(s) able but unable take up dimer [3H]digalacturonic
Batrachochytrium dendrobatidis is a globally distributed generalist pathogen that has driven many amphibian populations to extinction. The life cycle of B. two main cell types, motile zoospores, and sessile reproductive sporangia. When grown in nutrient-rich liquid medium, forms aggregates sporangia transition into monolayers on surfaces at the air-liquid interface. Pathogenic microorganisms use biofilms as mechanisms group interactions survive under harsh conditions absence suitable host....
Abstract Versatility in carbon source utilization assists Pseudomonas aeruginosa its adaptation to various niches. Recently, we characterized the role of malonate, an understudied source, quorum sensing regulation, antibiotic resistance, and virulence factor production P. . These results indicate that global responses malonate metabolism remain be uncovered. We leveraged a publicly available metabolomic dataset on human airway found as abundant glycerol, common metabolite for Here, explored...
Versatility in carbon source utilization is a major contributor to niche adaptation
Abstract Published Enzyme-Linked ImmunoSorbent Assay (ELISA) techniques to determine ricin, a castor (Ricinus communis) toxin, were developed for human sera, biological fluids, and tissue samples. This study was conducted adapt existing ELISA procedures soil extracts demonstrate its use. These modifications of published generic methods include elimination phosphate buffers in the extraction procedure addition bovine Fraction V albumin block nonspecific sites on microtiter plates. Using this...
Absorption, distribution, and biotransformation are 3 critical aspects affecting toxicant action in animals. In the present study, B6C3F1 mice (Mus musculus) were exposed for 28 d to contaminated feed that contained 1 of 5 different hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) concentrations: 0 mg/kg, 0.5 50 500 mg/kg. The authors quantified RDX its reductive transformation products hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine (MNX), hexahydro-1,3-dinitroso-5-nitro-1,3,5-triazine (DNX),...
ABSTRACT The skin and its microbiome are the first lines of defense against environmental stressors pathogens. symbiotic relationships between host within critical to health. In frogs, this research area is lacking especially in context their infection with Batrachochytrium dendrobatidis . B. a major fungal pathogen amphibians that has caused extinction hundreds amphibian species populations. While some frog known susceptible infections, others resistant, we hypothesized frogs plays role...