A5006220815- Wound Healing and Treatments
- Burn Injury Management and Outcomes
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Antibiotic Resistance in Bacteria
- Surgical Simulation and Training
- Hemoglobin structure and function
- Sepsis Diagnosis and Treatment
- Trauma Management and Diagnosis
- Anatomy and Medical Technology
- Trauma and Emergency Care Studies
- Pressure Ulcer Prevention and Management
- Microfluidic and Bio-sensing Technologies
- Surgical Sutures and Adhesives
- Heme Oxygenase-1 and Carbon Monoxide
- Injury Epidemiology and Prevention
- Cardiac Arrest and Resuscitation
- Abdominal Trauma and Injuries
- Reconstructive Surgery and Microvascular Techniques
- Dermatologic Treatments and Research
- Clinical Nutrition and Gastroenterology
- Augmented Reality Applications
- Petroleum Processing and Analysis
- Drug-Induced Adverse Reactions
- Thermal Regulation in Medicine
Texas Tech University
2016-2025
Texas Tech University Health Sciences Center
2016-2025
Lubbock Christian University
1995-2024
University Medical Center
2012-2024
Clinical Research Institute
2024
Augusta University Health
2023
Eskenazi Health
2023
University of California San Francisco Medical Center
2022
University Medical Center of El Paso
2018-2022
Atrium Health Wake Forest Baptist
2021
ABSTRACT The Pseudomonas aeruginosa quorum-sensing systems, las and rhl , control the production of numerous virulence factors. In this study, we have used burned-mouse model to examine contribution systems pathogenesis P. infections in burn wounds. Different mutants PAO1 that were defective lasR lasI or rhlI gene both genes utilized. following parameters infection examined: (i) lethality burned mouse, (ii) dissemination strain within body infected mouse (by determining numbers CFU liver...
Chronic wound infections are typically polymicrobial; however, most in vivo studies have focused on monospecies infections. This project was designed to develop an vivo, polymicrobial, biofilm-related, infected model order study multispecies biofilm dynamics and relation chronicity. Multispecies biofilms consisting of both Gram negative positive strains, as well aerobes anaerobes, were grown vitro then transplanted onto the wounds mice. These vitro-to-in multi-species transplants generated...
Pseudomonas aeruginosa produces multiple virulence factors and causes different types of infections. Previous clinical studies identified P. isolates that lack individual factors. However, the impact losing several simultaneously on in vivo is not completely understood. The cell-to-cell communication system, or quorum sensing (QS), controls production Animal using constructed QS mutants indicated loss system severely impacts aeruginosa. In this study, we tried to determine if deficiency...
Pelvic hemorrhage has been implicated as the cause of death in 50% patients who die following pelvic fractures. To establish correlates morbidity and mortality from fractures due to blunt trauma, we reviewed 236 treated during 4 years. The average age 144 men 92 women was 31.5 years, Injury Severity Score 21.3, blood requirement 5 units, hospital stay 16.8 days. One hundred fifty-two (64.4%) were injured motor vehicle accidents, 33 (14%) had vehicle-pedestrian 16 (6.8%) crush injuries, 12...
ABSTRACT Biofilms are bacterial communities residing within a polysaccharide matrix that associated with persistence and antibiotic resistance in chronic infections. We show the opportunistic pathogen Pseudomonas aeruginosa forms biofilms 8 h of infection thermally injured mice, demonstrating contribute to colonization acute infections as well. Using light, electron, confocal scanning laser microscopy, P. were visualized burned tissue surrounding blood vessels adipose cells. Although quorum...
ABSTRACT Gallium (Ga) is a semimetallic element that has demonstrated therapeutic and diagnostic-imaging potential in number of disease settings, including cancer infectious diseases. Gallium's biological actions stem from its ionic radius being almost the same as ferric iron (Fe 3+ ), whereby it can replace (Fe) Fe -dependent systems, such bacterial mammalian transporters -containing enzymes. Unlike , gallium (Ga ) cannot be reduced, when incorporated, inactivates reduction oxidation...
Diabetes mellitus (DM) affects 23.6 million people in the USA and approximately 20–25% of diabetic patients will develop foot ulceration during course their disease. Up to a quarter these infections that necessitate amputation. Although many studies report rates antibiotic resistant have increased dramatically DM population over last decade, our knowledge there been no reports directly comparing versus non-DM wounds. We performed retrospective study wound 41 those 74 test hypothesis with...
Early excision and autografting are standard care for deeper burns. However, donor sites a source of significant morbidity. To address this, the ReCell® Autologous Cell Harvesting Device (ReCell) was designed use at point-of-care to prepare noncultured, autologous skin cell suspension (ASCS) capable epidermal regeneration using minimal skin. A prospective study conducted evaluate clinical performance ReCell vs meshed split-thickness grafts (STSG, Control) treatment deep partial-thickness...
NovaSil clay (NS) provides significant protection from the adverse effects of aflatoxins (AFs) in multiple animal species by decreasing bioavailability gastrointestinal tract. It is postulated that NS can be safely added to human diets diminish exposure and health risks AF contaminated food. To determine safety tolerance humans establish dosimetry protocols for long-term efficacy studies, a randomized double-blinded phase I clinical trial was conducted. Volunteers (20–45 yr age), were...
Compared with nonsurgical management or delayed repair, early fracture fixation can reduce the incidence of pulmonary complications in patients long-bone fractures lower extremities. Blunt trauma victims often have multiple nonskeletal injuries that might influence risk complications, and when head are present it has been a common practice to delay nonemergent operations for several days protect injured brain. We conducted retrospective review 114 whose included neck shaft femur tibia...
ABSTRACT Quorum sensing (QS) is a cell density-dependent signaling mechanism used by many bacteria to control gene expression. Several recent reports indicate that the molecules (autoinducers) mediate QS in Pseudomonas aeruginosa may also modulate expression host cells; however, mechanisms are largely unknown. Here we show two P. autoinducers, N -3-oxododecanoyl-homoserine lactone and -butyryl-homoserine lactone, can both enter eukaryotic cells activate artificial chimeric transcription...
Abstract Introduction Over the past decade, novel options have provided promising approaches into conundrum of how to best treat full thickness burns. The primary objective proper resuscitation and adequate skin coverage is a balancing act between fighting infections, insensible loss, metabolic imbalances. Previously, dermal substitutes been traditional treatment option for burns, but availability acquisition delays treatment, increasing risk mortality despite advances in options. Recent...
Abstract Introduction Predicting discharge disposition for burn patients with large total body surface area (TBSA) is essential to optimizing care and improving long-term outcomes. Patient recovery, expectations, goal setting require early physician planning, but recovery rarely based solely on TBSA. A multidisciplinary approach, paired simple yet comprehensive assessment tools, can enhance patient discussions family preparedness. The Activity Measure Post-Acute Care Six-Clicks, a functional...
Abstract Introduction Burn injuries are some of the most severe that enter hospitals. Length stay (LOS) is often difficult to predict in these patients, and variability individual responses care makes it estimate burn recovery. mortality prediction well-established, but as survival rates rapidly increase, formulas have be readjusted. Physical therapists gauge mobility with a tool known AM-PAC 6-clicks on scale 6 24, lower scores implying worse mobility. These tools offer an interprofessional...
Abstract Introduction In the management of burn injuries, accurate fluid resuscitation plays a critical role in restoring intravascular volume and avoiding overload complications, which can escalate mortality morbidity significantly. Protocols such as 1960s Parkland formula have reduced yet differing body mass indices (BMI) patients pose physiological impacts that may not be accounted for by these formulas. This study investigates influence BMI on fluids administered first 24 hours...
Pseudomonas aeruginosa is a gram-negative opportunistic pathogen that causes severe infections in patients with burns. The P regulatory gene, lasR, regulates the expression of several virulence factors. specific lasR isogenic mutant, PAO-R1, defective synthesis 2 elastases (LasB and LasA) produces low levels exotoxin A alkaline proteases. In this study, we used burned mouse model to examine role pathogenesis infections. We have examined following aspects infections: 1) lethality mouse, 2)...