A5050311332- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Plant Parasitism and Resistance
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Transgenic Plants and Applications
- Banana Cultivation and Research
- Plant pathogens and resistance mechanisms
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Phytase and its Applications
- Canadian Identity and History
- Toxin Mechanisms and Immunotoxins
- Studies on Chitinases and Chitosanases
- Migration, Identity, and Health
- Protein Hydrolysis and Bioactive Peptides
- Lipid Membrane Structure and Behavior
- Employment, Labor, and Gender Studies
- Plant Disease Resistance and Genetics
- Sugarcane Cultivation and Processing
Centre for Plant Biotechnology and Genomics
2012-2021
Universidad Politécnica de Madrid
2012-2021
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2013-2021
Universidad de Sevilla
2000-2017
Universidad Complutense de Madrid
2004-2012
Molina Center for Energy and the Environment
2010
Polytechnic University of Puerto Rico
2003
Agricultural Experiment Station
1991
Cornell University
1991
Plant (United States)
1991
Bacterial isolates from oak trees in Spain and Britain, showing symptoms of bark canker Acute Oak Decline (AOD), respectively, were examined by a polyphasic approach. Both 16S rRNA gene sequencing multilocus sequence analysis (MLSA), based on partial sequences gyrB, rpoB, infB atpD genes, revealed that the separated into two genetic groups according to their origin. Their closest phylogenetic relative was Brenneria quercina, causal agent drippy nut disease oak, which clustered distant other...
The bacterial pathogen Pseudomonas syringae pv tomato DC3000 suppresses plant innate immunity with effector proteins injected by a type III secretion system (T3SS). cysteine protease HopN1, which reduces the ability of to elicit programmed cell death in non-host tobacco, was found also suppress production defence-associated reactive oxygen species (ROS) and callose when delivered fluorescens heterologously expressing P. T3SS. Purified His(6) -tagged HopN1 used identify PsbQ, member evolving...
ABSTRACT Plant-beneficial microorganisms are gaining importance for sustainable plant production and phytosanitary practices. Yet there is a lack of computational approaches targeting bacterial traits associated with growth-promotion (PGP), which hinders the in-silico identification, comparison, selection phytostimulatory strains. To address this problem, we have developed new web resource PLaBAse (v1.01, http://plabase.informatik.uni-tuebingen.de/pb/plabase.php ), provides number services,...
Dickeya dadantii is a plant-pathogenic enterobacterium responsible for the soft rot disease of many plants economic importance. We present here sequence strain 3937, widely used as model system research on molecular biology and pathogenicity this group bacteria.
Dickeya dadantii 3937 (ex Erwinia chrysanthemi ), a member of the Enterobacteriaceae , causes soft rot in many economically important crops. A successful pathogen has to reach interior plant order cause disease. To study role motility and chemotaxis pathogenicity D. 3937, genes involved chemotactic signal transduction system ( cheW cheB cheY cheZ ) structure flagellar motor motA were mutagenized. All mutant strains grew like wild-type culture media, production secretion pectolytic enzymes...
Pseudomonas savastanoi pv. is a tumour-inducing pathogen of Olea europaea L. causing olive knot disease. Bioinformatic analysis the draft genome sequence strain NCPPB 3335, which encodes 5232 predicted coding genes on total length 5856 998 bp and 57.12% G + C, revealed large degree conservation with syringae phaseolicola 1448A P. tabaci 11528. However, 3335 contains twelve variable genomic regions, are absent in all previously sequenced strains. Various features that could contribute to...
We investigated the role in pathogenesis of bacterial resistance to plant antimicrobial peptides. The sapA sapF (for sensitive peptides) operon from pathogenic bacterium Erwinia chrysanthemi has been characterized. It five open reading frames that are closely related (71% overall amino acid identity) and same order as those Salmonella typhimurium. An E. sap mutant strain was constructed by marker exchange. This more than wild type wheat α-thionin snakin-1, which is most abundant peptide...
Agrobacterium tumefaciens biovar 3 causes both crown gall and root decay of grapes. All strains, regardless their tumorigenicity, produce in culture a single polygalacturonase with pI around 4.5. A. strain CG49 was mutagenized Tn5 by using pSUP2021 as suicide vector. A mutant strain, CG50, lacking activity isolated. The mutation due to insertion an 8.5-kb EcoRI fragment that also contained the structural gene. polygalacturonase-encoding pehA gene cloned Escherichia coli plasmid pBluescript...
Pseudomonas fluorescens strain PICF7 is a native endophyte of olive roots. Previous studies have shown this motile, Gram-negative, non-sporulating bacterium an effective biocontrol agent against the soil-borne fungus Verticillium dahliae, causal one most devastating diseases for (Olea europaea L.) cultivation. Here, we announce and describe complete genome sequence consisting circular chromosome 6,136,735 bp that encodes 5,567 protein-coding genes 88 RNA-only encoding genes. Genome analysis...
T346Hunter (Type Three, Four and Six secretion system Hunter) is a web-based tool for the identification localisation of type III, IV VI systems (T3SS, T4SS T6SS, respectively) clusters in bacterial genomes. Non-flagellar T3SS (NF-T3SS) T6SS are complex molecular machines that deliver effector proteins from cells into environment or other eukaryotic prokaryotic cells, with significant implications pathogenesis strains encoding them. Meanwhile, more functionally diverse system, which involved...
Chemosensory pathways are among the most abundant prokaryotic signal transduction systems, allowing bacteria to sense and respond environmental stimuli. Signaling is typically initiated by binding of specific molecules ligand domain (LBD) chemoreceptor proteins (CRs). Although CRs play a central role in plant-microbiome interactions such as colonization infection, little known about their phylogenetic ecological specificity. Here, we analyzed 82,277 CR sequences from 11,806 representative...
Purified chestnut cystatin inhibited the growth of phytopathogenic fungi Botrytis cinerea, Colletotrichum graminicola, and Septoria nodorum, but not that saprophyte Trichoderma viride. Furthermore, strongly affected protease activity B. cinerea had no effect on T. These results suggest contributes to plant defense against fungi.
Jasmonate is a key signalling compound in plant defence that synthesized wounded tissues. In this work, we have found molecule also strong chemoattractant for the phythopathogenic bacteria Dickeya dadantii (ex-Erwinia chysanthemi). Jasmonic acid induced expression of subset bacterial genes possibly involved virulence/survival apoplast and cells pre-treated with jasmonate showed increased virulence chicory Saintpaulia leaves. We tissue wounding spread through leaf surface. Moreover,...
Pseudomonas syringae pv tomato DC3000 (Pto) is the causal agent of bacterial speck tomato, which leads to significant economic losses in this crop. Pto inhabits phyllosphere, where pathogen highly exposed light, among other environmental factors. Light represents a stressful condition and acts as source information associated with different plant defence levels. Here, we analysed presence both blue red light photoreceptors group Pseudomonas. In addition, studied effect white, on features...
Summary Recent scenarios of fresh produce contamination by human enteric pathogens have resulted in severe food‐borne outbreaks, and a new paradigm has emerged stating that some human‐associated bacteria can use plants as secondary hosts. As consequence, there been growing concern the scientific community about these interactions not yet elucidated. Since this is relatively area, lack strategies to address problem illnesses due ingestion fruits vegetables. In present study, we performed...
There is substantive evidence that chemotaxis a key requisite for efficient pathogenesis in plant pathogens. However, information regarding particular bacterial chemoreceptors and the specific signal they sense scarce. Our work shows phytopathogenic bacterium Pseudomonas syringae pv. tomato mediates not only but also control of pathogenicity through perception abundant amino acids Asp Glu. We describe specificity l - d -Asp -Glu by PsPto-PscA chemoreceptor involvement this regulation...
Ralstonia solanacearum K60 was mutagenized with the transposon Tn5, and two mutants, M2 M88, were isolated. Both mutants selected based on their increased sensitivity to thionins, they had Tn5 insertion in same gene, 34 bp apart. Sequence analysis of interrupted gene showed clear homology rfaF from Escherichia coli Salmonella typhimurium (66% similarity), which encodes a heptosyltransferase involved synthesis lipopolysaccharide (LPS) core. Mutants M88 an altered LPS electrophoretic pattern,...
The effects of five antipathogenic plant peptides, wheat α‐thionin, potato PTH1 defensin, barley LTP2 lipid transfer protein, and tuber DL1 DL2 defensins, have been tested against phospholipid vesicles (liposomes). Wheat thionin very actively induces aggregation leakage negatively charged vesicles. displays the same activities, although to a limited extent. Under certain conditions induce vesicle aggregation, but not leakage. Potato defensin failed show any effect on liposomes. peptides...
The recombinant barley cystatin Hv-CPI inhibited the growth of three phytopathogenic fungi (Botrytis cinerea, Colletotrichum graminicola, and Plectosphaerella cucumerina) saprotrophic fungus Trichoderma viride. Several mutants were generated by polymerase chain reaction approaches both their anti-fungal cysteine-proteinase inhibitory properties investigated. Point R 38 →G, Q 63 →L, →P diminished capacity for inhibiting papain cathepsin B, retaining antifungal properties. However, mutant C 68...